Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.

abstract：:We determined the entire nucleotide sequence of phiSa2958-carrying Panton-Valentine leukocidin (PVL) gene, which was lysogenized in a sequence type 5 staphylococcal cassette chromosome mec (SCCmec) type II strain of methicillin-resistant Staphylococcus aureus (MRSA). Based on the nucleotide sequences of PVL phages, we...

journal_title：Journal of clinical microbiology

authors： Ma XX,Ito T,Kondo Y,Cho M,Yoshizawa Y,Kaneko J,Katai A,Higashiide M,Li S,Hiramatsu K

更新日期：2008-10-01 00:00:00

abstract：:A gas-liquid chromatographic method which requires no chloroform extraction of the split products has been investigated for the detection of hippurate hydrolysis by Campylobacter spp. This technique gave better reproducibility than other tests also used in this study and allows the routine use of the gas-liquid chroma...

journal_title：Journal of clinical microbiology

authors： Bär W,Fricke G

更新日期：1987-09-01 00:00:00

abstract：:Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

journal_title：Journal of clinical microbiology

authors： Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:A collection of 54 unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid samples from neonates and 60 unrelated strains isolated from carriers that had been previously studied by multilocus enzyme electrophoresis (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Cli...

journal_title：Journal of clinical microbiology

authors： Chatellier S,Ramanantsoa C,Harriau P,Rolland K,Rosenau A,Quentin R

更新日期：1997-10-01 00:00:00

abstract：:A transrectal prostate biopsy is the most common procedure used to establish the diagnosis of prostate cancer. Prior to biopsy, patients are commonly given ciprofloxacin for prophylaxis. However, a complication of the procedure is infection with ciprofloxacin-resistant organisms, in particular resistant Escherichia co...

journal_title：Journal of clinical microbiology

authors： Liss MA,Nakamura KK,Peterson EM

更新日期：2013-01-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suita...

journal_title：Journal of clinical microbiology

authors： Hrncirova K,Lengerova M,Kocmanova I,Racil Z,Volfova P,Palousova D,Moulis M,Weinbergerova B,Winterova J,Toskova M,Pospisilova S,Mayer J

更新日期：2010-09-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for detection of feline leukemia virus (FeLV) p27 in saliva was tested for its accuracy and sensitivity in diagnosing FeLV infections. Saliva and serum samples from 564 clinical cases were tested with a 99.2% specificity. The overall accuracy of the saliva ELISA reactive to...

journal_title：Journal of clinical microbiology

authors： Lewis MG,Wright KA,Lafrado LJ,Shanker PJ,Palumbo NE,Lemoine ED,Olsen RG

更新日期：1987-07-01 00:00:00

abstract：:Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue f...

journal_title：Journal of clinical microbiology

authors： Okabayashi T,Sasaki T,Masrinoul P,Chantawat N,Yoksan S,Nitatpattana N,Chusri S,Morales Vargas RE,Grandadam M,Brey PT,Soegijanto S,Mulyantno KC,Churrotin S,Kotaki T,Faye O,Faye O,Sow A,Sall AA,Puiprom O,Chaichana P,

更新日期：2015-02-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00

abstract：:The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resu...

journal_title：Journal of clinical microbiology

authors： Vatanshenassan M,Boekhout T,Lass-Flörl C,Lackner M,Schubert S,Kostrzewa M,Sparbier K

更新日期：2018-08-27 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:Two new T-cell-based reporter cell lines were established to measure human immunodeficiency virus type 1 (HIV-1) infectivity. One cell line naturally expresses CD4 and CXCR4, making it susceptible to X4-tropic viruses, and the other cell line, in which a CCR5 expression vector was introduced, is susceptible to both X4...

journal_title：Journal of clinical microbiology

authors： Chiba-Mizutani T,Miura H,Matsuda M,Matsuda Z,Yokomaku Y,Miyauchi K,Nishizawa M,Yamamoto N,Sugiura W

更新日期：2007-02-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:Recent molecular studies have shown Mycobacterium porcinum, recovered from cases of lymphadenitis in swine, to have complete 16S rDNA sequence identity and >70% DNA-DNA homology with human isolates within the M. fortuitum third biovariant complex. We identified 67 clinical and two environmental isolates of the M. fort...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown-Elliott BA,Wilson RW,Mann L,Hall L,Zhang Y,Jost KC Jr,Brown JM,Kabani A,Schinsky MF,Steigerwalt AG,Crist CJ,Roberts GD,Blacklock Z,Tsukamura M,Silcox V,Turenne C

更新日期：2004-12-01 00:00:00

abstract：:Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...

journal_title：Journal of clinical microbiology

authors： Banerjee D,Michael J,Schmitt B,Salimnia H,Mhaissen N,Goldfarb DM,Lachance P,Faron ML,Aufderheide T,Ledeboer N,Weissfeld A,Selvarangan R

更新日期：2020-06-24 00:00:00

abstract：:Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern an...

journal_title：Journal of clinical microbiology

authors： Migheli Q,Balmas V,Harak H,Sanna S,Scherm B,Aoki T,O'Donnell K

更新日期：2010-04-01 00:00:00

abstract：:Roche Molecular Systems recently released two PCR-based assays, AMPLICOR and LINEAR ARRAY (LA), for the detection and genotyping, respectively, of human papillomaviruses (HPVs). The manual specimen processing method recommended for use with both assays, AmpliLute, can be time-consuming and labor-intensive and is open ...

journal_title：Journal of clinical microbiology

authors： Stevens MP,Rudland E,Garland SM,Tabrizi SN

更新日期：2006-07-01 00:00:00

abstract：:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). ...

journal_title：Journal of clinical microbiology

authors： Twin J,Taylor N,Garland SM,Hocking JS,Walker J,Bradshaw CS,Fairley CK,Tabrizi SN

更新日期：2011-03-01 00:00:00

abstract：:We evaluated the abilities of three different avian influenza A viruses to attenuate the wild-type human influenza A/Korea/1/82 (H3N2) virus in squirrel monkeys, chimpanzees, and adult seronegative human volunteers. Two of these, avian influenza A/Mallard/NY/78 and A/Mallard/Alberta/76 viruses, appeared to be satisfac...

journal_title：Journal of clinical microbiology

authors： Snyder MH,Clements ML,Herrington D,London WT,Tierney EL,Murphy BR

更新日期：1986-09-01 00:00:00

abstract：:A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and par...

journal_title：Journal of clinical microbiology

authors： Iltis JP,Castellano GA,Gerber P,Le C,Vujcic LK,Quinnan GV Jr

更新日期：1982-11-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:Chlamydia pneumoniae is an important human respiratory pathogen. Classification of C. pneumoniae isolates into distinguishable serovars or genotypes has not yet been reported. To determine whether antigenic or molecular variants among C. pneumoniae isolates exist, six strains were studied via immunoblot analysis and D...

journal_title：Journal of clinical microbiology

authors： Jantos CA,Heck S,Roggendorf R,Sen-Gupta M,Hegemann JH

更新日期：1997-03-01 00:00:00

abstract：:The etiological agents of diarrhea in Vientiane, Lao People's Democratic Republic (Lao PDR), were studied in the period from October 1996 to August 1997. A total of 880 patients with diarrhea visiting medical facilities were examined for Shigella, Salmonella, diarrheagenic Escherichia coli, vibrio, Aeromonas, Campylob...

journal_title：Journal of clinical microbiology

authors： Yamashiro T,Nakasone N,Higa N,Iwanaga M,Insisiengmay S,Phounane T,Munnalath K,Sithivong N,Sisavath L,Phanthauamath B,Chomlasak K,Sisulath P,Vongsanith P

更新日期：1998-08-01 00:00:00

abstract：:This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here proc...

journal_title：Journal of clinical microbiology

authors： Gronowski AM,Copper S,Baorto D,Murray PR

更新日期：2000-06-01 00:00:00

abstract：:The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staini...

journal_title：Journal of clinical microbiology

authors： Ohm-Smith MJ,Nassos PS,Haller BL

更新日期：2004-07-01 00:00:00

abstract：:Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble an...

journal_title：Journal of clinical microbiology

authors： Farshy CE,Klein GC,Feeley JC

更新日期：1978-04-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:Totals of 102 and 56 Clostridium difficile type 078 strains of human and porcine origins, respectively, from four European countries were investigated by an optimized multilocus variable-number tandem-repeat analysis (MLVA) and for tetracycline susceptibility. Eighty-five percent of all isolates were genetically relat...

journal_title：Journal of clinical microbiology

authors： Bakker D,Corver J,Harmanus C,Goorhuis A,Keessen EC,Fawley WN,Wilcox MH,Kuijper EJ

更新日期：2010-10-01 00:00:00