Spread of Escherichia coli strains with high-level cefotaxime and ceftazidime resistance between the community, long-term care facilities, and hospital institutions.

Abstract:

:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated strains. The principal cluster was isolated from urine, wound, blood, and other samples in three hospitals, eight nursing homes, and a community healthcare center. This cluster was associated with both nosocomial (65%) and community-acquired (35%) infections. Most strains were resistant to ciprofloxacin, gentamicin, tobramycin, cefepime, amoxicillin-clavulanic acid, and trimethoprim-sulfamethoxazole but were susceptible to imipenem. All isolates from the four clusters expressed the extended-spectrum beta-lactamase (ESBL) CTX-M-15. This enzyme was also present in 8 (30.8%) of the 26 unrelated isolates. The other ESBLs, CTX-M-14 and CTX-M-32, were detected in five and seven cases, respectively, but they were detected in individual E. coli isolates only. In three clusters, blaCTX-M-15 alleles were linked to an ISEcp1-like element, while in eight strains of cluster II an IS26 element preceded the blaCTX-M-15 allele. An additional pool of resistance genes included tetA, drfA14 or dfrA17, sul1 or sul2, aac(6')Ib, and aac(3)IIb. All except one of the 27 isolates tested for genetic virulence markers harbored the same three virulence genes: iutA and fyuA (siderophores), and traT (serum survival factor). Epidemic or occasional isolates of cefotaxime- and ceftazidime-resistant E. coli can spread between distinct health facilities including hospitals, community health centers, and long-term care centers.

journal_name

J Clin Microbiol

authors

Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

doi

10.1128/JCM.00447-06

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

2359-66

issue

7

eissn

0095-1137

issn

1098-660X

pii

44/7/2359

journal_volume

44

pub_type

杂志文章
  • Detection of point mutations associated with resistance of Helicobacter pylori to clarithromycin by hybridization in liquid phase.

    abstract::When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.11.3285-3290.1998

    authors: Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

    更新日期:1998-11-01 00:00:00

  • Implementation of Semiautomated Antimicrobial Susceptibility Interpretation Hardware for Nontuberculous Mycobacteria May Overestimate Susceptibility.

    abstract::Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01756-18

    authors: Rockland M,Ruth MM,Aalders N,Pennings L,Hoefsloot W,Wattenberg M,van Ingen J

    更新日期:2019-03-28 00:00:00

  • Survival, Persistence, and Isolation of the Emerging Multidrug-Resistant Pathogenic Yeast Candida auris on a Plastic Health Care Surface.

    abstract::The emerging multidrug-resistant pathogenic yeast Candida auris represents a serious threat to global health. Unlike most other Candida species, this organism appears to be commonly transmitted within health care facilities and causes health care-associated outbreaks. To better understand the epidemiology of this emer...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00921-17

    authors: Welsh RM,Bentz ML,Shams A,Houston H,Lyons A,Rose LJ,Litvintseva AP

    更新日期:2017-10-01 00:00:00

  • Comparative evaluation of four commercially available monoclonal antibodies for culture confirmation of herpes simplex virus infection.

    abstract::Four fluorescein isothiocyanate-labelled monoclonal antibody typing reagents were compared for intensity of fluorescence and sensitivity in confirming herpes simplex virus (HSV) in cell culture. A total of 125 (50 HSV type 1 [HSV-1] and 75 HSV-2) specimens positive for HSV were concurrently stained with type-specific ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.7.1874-1875.1992

    authors: Johnston SL,Wellens K

    更新日期:1992-07-01 00:00:00

  • The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product.

    abstract::The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.3.548-552.1999

    authors: Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

    更新日期:1999-03-01 00:00:00

  • Viral studies on human brain culture retrieved from cold storage.

    abstract::Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.1.5.405-410.1975

    authors: Lennette ET

    更新日期:1975-05-01 00:00:00

  • Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand.

    abstract::Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00680-06

    authors: McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

    更新日期:2006-12-01 00:00:00

  • Meningitis and endocarditis caused by Campylobacter fetus after raw-liver ingestion.

    abstract::We report Campylobacter fetus meningitis associated with endocarditis in a 75-year-old diabetic man after he consumed raw liver. C. fetus was isolated from blood samples and cerebrospinal fluid. Cure was obtained with combined intravenous imipenem-gentamicin for 4 weeks; no relapse occurred after 6 months of follow-up...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00631-13

    authors: Suy F,Le Dû D,Roux AL,Hanachi M,Dinh A,Crémieux AC

    更新日期:2013-09-01 00:00:00

  • Serum antibody responses in naturally occurring influenza A virus infection determined by enzyme-linked immunosorbent assay, hemagglutination inhibition, and complement fixation.

    abstract::Serum antibody responses to influenza A virus infection were examined in 388 normal subjects during a trial of chemoprophylaxis in an outbreak of influenza A in 1980-1981 in which both A/H1N1 and A/H3N2 viruses circulated. Paired serum specimens obtained over a 6-week period were tested for antibodies to both A/H1N1 a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.6.1345-1350.1983

    authors: Madore HP,Reichman RC,Dolin R

    更新日期:1983-12-01 00:00:00

  • Determination of bovine rotavirus G and P serotypes in italy by PCR.

    abstract::Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.12.3879-3882.1999

    authors: Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

    更新日期:1999-12-01 00:00:00

  • Molecular analysis of genes encoding outer surface protein C (OspC) of Borrelia burgdorferi sensu lato: relationship to ospA genotype and evidence of lateral gene exchange of ospC.

    abstract::It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.7.1860-1866.1995

    authors: Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

    更新日期:1995-07-01 00:00:00

  • Sequence analysis of the p1 adhesin gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2008 to 2009.

    abstract::The p1 genes of 60 Mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. An AGT trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. In addition, a novel p1 gene variant named 2c was identified in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00105-11

    authors: Zhao F,Cao B,Li J,Song S,Tao X,Yin Y,He L,Zhang J

    更新日期:2011-08-01 00:00:00

  • Continuous high-speed rolling versus centrifugation for detection of herpes simplex virus.

    abstract::Specimens submitted for diagnosis of herpes simplex virus infections were inoculated into shell vials and conventional culture tubes. Inoculated culture tubes were incubated with rolling at 96 rpm. Immunoperoxidase (IP) staining and cytopathic effects (CPE) were used to detect positive cultures. At 24 h, 42 (53%) of t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.12.2884-2886.1989

    authors: Hughes JH,Hamparian VV,Mavromoustakis CT

    更新日期:1989-12-01 00:00:00

  • Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy.

    abstract::Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.8.4245-4248.2005

    authors: Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

    更新日期:2005-08-01 00:00:00

  • Occurrence of norovirus infections unrelated to norovirus outbreaks in an asymptomatic food handler population.

    abstract::Norovirus (NV) is the most common causative agent of nonbacterial gastroenteritis. Reports of surveillance of NV in facilities that reported outbreaks are frequently found in publications, but reports of that in facilities without outbreaks are not found. We investigated the molecular epidemiology of NV isolates deriv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00305-08

    authors: Okabayashi T,Yokota S,Ohkoshi Y,Ohuchi H,Yoshida Y,Kikuchi M,Yano K,Fujii N

    更新日期:2008-06-01 00:00:00

  • Neglecting genetic diversity hinders timely diagnosis of Cryptococcus infections.

    abstract::Over a decade ago the first cryptococcal lateral flow assay (LFA) became available. This affordable point-of-care test has undeniably led to tremendous improvements in early detection of cryptococcosis (1,2). However, from several studies it must be concluded that the reliability of recently introduced LFA's falls sho...

    journal_title:Journal of clinical microbiology

    pub_type: 信件

    doi:10.1128/JCM.02837-20

    authors: Shi D,Haas PJ,Boekhout T,Hahn RC,Hagen F

    更新日期:2021-01-20 00:00:00

  • Application of 16S rRNA gene PCR to study bowel flora of preterm infants with and without necrotizing enterocolitis.

    abstract::The purpose of the present study was to determine the extent to which bacteria not detected by culture contribute to the microbial flora of the bowel of preterm infants with and without neonatal necrotizing enterocolitis (NEC). Fecal samples from 32 preterm infants in special care baby units including samples from 10 ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.10.2506-2510.1996

    authors: Millar MR,Linton CJ,Cade A,Glancy D,Hall M,Jalal H

    更新日期:1996-10-01 00:00:00

  • Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis.

    abstract::Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.8.2007-2010.1996

    authors: Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

    更新日期:1996-08-01 00:00:00

  • Lessons in public (mis)communication about the laboratory from the COVID-19 pandemic.

    abstract::The COVID-19 pandemic has put the clinical laboratory in the spotlight. The news media is regularly seeking out interviews with microbiologists, infectious disease specialists, and pathologists. Increased public exposure offers opportunities to improve how laboratory professionals communicate our insights. We can emph...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02917-20

    authors: Mazer BL

    更新日期:2021-01-21 00:00:00

  • Characterization of clinically significant strains of coagulase-negative staphylococci.

    abstract::On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.2.258-269.1983

    authors: Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

    更新日期:1983-08-01 00:00:00

  • Field Evaluation of Four Rapid Tests for Diagnosis of HIV Infection in Panama.

    abstract::Four HIV rapid tests were subjected to field validation in Panama and compared to an enzyme-linked immunosorbent assay/Multispot-based testing algorithm. The sensitivities of Determine, Uni-Gold, SD Bioline, and INSTI were 99.8%. The specificities of Determine, SD-Bioline, and Uni-Gold were 100%, and the specificity o...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02654-15

    authors: Juarez SI,Nuñez AE,Aranda MM,Mojica D,Kim AA,Parekh B

    更新日期:2016-04-01 00:00:00

  • Cross-border emergence of Escherichia coli producing the carbapenemase NDM-5 in Switzerland and Germany.

    abstract::A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5-producing E. coli isolates were highly resistant to β-lactams including the novel ß-lactam...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02238-20

    authors: Chakraborty T,Sadek M,Yao Y,Imirzalioglu C,Stephan R,Poirel L,Nordmann P

    更新日期:2020-12-23 00:00:00

  • Multicenter evaluation of five commercial rubella virus immunoglobulin G kits which report in international units per milliliter.

    abstract::In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.3.633-641.1992

    authors: Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

    更新日期:1992-03-01 00:00:00

  • Broth microdilution and disk diffusion tests for susceptibility testing of Pasteurella species isolated from human clinical specimens.

    abstract::Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.5.2485-2488.2005

    authors: Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

    更新日期:2005-05-01 00:00:00

  • Genetic similarity of Candida albicans strains from vaginitis patients and their partners.

    abstract::The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.1.39-46.1993

    authors: Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

    更新日期:1993-01-01 00:00:00

  • Mycobacterium avium complex, an emerging pathogen in Massachusetts.

    abstract::We report a study of 1,953 patients whose laboratory records from 1972 through 1983 at the Massachusetts Mycobacteria Reference Laboratory indicated the isolation of Mycobacterium avium complex (MAC) organisms. At least one clinical specimen from each patient during this period exhibited the organism. The incidence of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.1.9-12.1985

    authors: du Moulin GC,Sherman IH,Hoaglin DC,Stottmeier KD

    更新日期:1985-07-01 00:00:00

  • Outbreak of carbapenem-resistant Acinetobacter baumannii producing the carbapenemase OXA-23 in a tertiary care hospital of Papeete, French Polynesia.

    abstract::Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.9.4826-4829.2005

    authors: Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

    更新日期:2005-09-01 00:00:00

  • Quantitative measurement of anti-SARS-CoV-2 antibodies: Analytical and clinical evaluation.

    abstract::The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection. While most serologic assays are qualitativ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03149-20

    authors: Higgins V,Fabros A,Kulasingam V

    更新日期:2021-01-22 00:00:00

  • Comparison of various blood compartments and reporting units for the detection and quantification of Epstein-Barr virus in peripheral blood.

    abstract::Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02308-06

    authors: Hakim H,Gibson C,Pan J,Srivastava K,Gu Z,Bankowski MJ,Hayden RT

    更新日期:2007-07-01 00:00:00

  • Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strains.

    abstract::By use of the nearly perfectly colinear genomes of Rickettsia conorii and Rickettsia prowazekii, we compared the usefulness of three types of sequences for typing of R. conorii isolates: (i) 5 variable coding genes comprising the 16S ribosomal DNA, gltA, ompB, and sca4 (gene D) genes, which are present in both genomes...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.12.5757-5766.2004

    authors: Fournier PE,Zhu Y,Ogata H,Raoult D

    更新日期:2004-12-01 00:00:00