Induction of procoagulant activity in human leukocytes with lipoglycans from mycoplasmas.

abstract：:Analysis of 177 methicillin-resistant Staphylococcus aureus (MRSA) strains for the presence of egc and tst revealed that 60 strains carried at least one of the tested genes. MRSA strains were classified by pulsed-field gel electrophoresis into four clones belonging to agr groups I and III. Toxin genotypes were related...

journal_title：Journal of clinical microbiology

authors： Chini V,Dimitracopoulos G,Spiliopoulou I

更新日期：2006-05-01 00:00:00

abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...

journal_title：Journal of clinical microbiology

authors： Kurzai O,Heinz WJ,Sullivan DJ,Coleman DC,Frosch M,Mühlschlegel FA

更新日期：1999-05-01 00:00:00

abstract：:Sequence analysis of the penA gene, encoding penicillin-binding protein 2 (PBP2), in 30 penicillin-intermediate (PenI) Neisseria meningitidis strains showed altered gene sequences due to the translocation of exogenous DNA blocks derived from commensal neisseriae, which are known to have PBP2 proteins with decreased af...

journal_title：Journal of clinical microbiology

authors： Stefanelli P,Carattoli A,Neri A,Fazio C,Mastrantonio P

更新日期：2003-10-01 00:00:00

abstract：:Eight commercial enzyme-linked immunosorbent assays (ELISAs) were used to test sera taken from 102 patients in whom Helicobacter pylori infection status had been determined by means of biopsy culture, PCR, histology, and urease production and by 13C urea breath test. By those means, 61 patients had been found to be in...

journal_title：Journal of clinical microbiology

authors： Meijer BC,Thijs JC,Kleibeuker JH,van Zwet AA,Berrelkamp RJ

更新日期：1997-01-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Sampson-Scherer J

更新日期：1977-03-01 00:00:00

abstract：:The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivit...

journal_title：Journal of clinical microbiology

authors： Wu TC,Zaza S,Callaway J

更新日期：1989-08-01 00:00:00

abstract：:Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...

journal_title：Journal of clinical microbiology

authors： Effelsberg N,Stegger M,Peitzmann L,Altinok O,Coombs GW,Pichon B,Kearns A,Randad PR,Heaney CD,Bletz S,Schaumburg F,Mellmann A

更新日期：2020-12-17 00:00:00

abstract：:Specific antibodies against human immunodeficiency virus (HIV), usually used for diagnosis, almost invariably become detectable within 3 months of exposure. We report on a patient whose HIV infection was identified early by a combined antigen/antibody test, but seroconversion did not occur for 7 months, until the impl...

journal_title：Journal of clinical microbiology

authors： Chin BS,Lee SH,Kim GJ,Kee MK,Suh SD,Kim SS

更新日期：2007-05-01 00:00:00

abstract：:An accurate, rapid, and inexpensive method was developed for detecting and enumerating bacteria adherent to Foley urinary catheters based on malachite green staining of acridine orange-prestained specimens. This method has proven to be quick and reliable and will find application in quantitative studies of biomaterial...

journal_title：Journal of clinical microbiology

authors： Ladd TI,Schmiel D,Nickel JC,Costerton JW

更新日期：1985-06-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:Two national surveys were conducted to describe the incidence and prevalence of Enterobacter aerogenes in 21 Belgian hospitals in 1996 and 1997 and to characterize the genotypic diversity and the antimicrobial resistance profiles of clinical strains of E. aerogenes isolated from hospitalized patients in Belgium in 199...

journal_title：Journal of clinical microbiology

authors： De Gheldre Y,Struelens MJ,Glupczynski Y,De Mol P,Maes N,Nonhoff C,Chetoui H,Sion C,Ronveaux O,Vaneechoutte M,Groupement pour le Dépistage, l'Etude et la Prevention des Infections Hospitalières (GDEPIH-GOSPIZ).

更新日期：2001-03-01 00:00:00

abstract：:Specimens of colonic mucosa from two pigs with diarrhea were examined by light and electron microscopy. The epithelial surfaces of both pigs were extensively colonized by large spirochetes morphologically compatible with Treponema hyodysenteriae or Treponema innocens. The microorganisms were intimately attached end-on...

journal_title：Journal of clinical microbiology

authors： Jacques M,Girard C,Higgins R,Goyette G

更新日期：1989-05-01 00:00:00

abstract：:A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...

journal_title：Journal of clinical microbiology

authors： Stills HF Jr,Hook RR Jr,Kinden DA

更新日期：1989-11-01 00:00:00

abstract：:In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged storage at room tempera...

journal_title：Journal of clinical microbiology

authors： Shima K,Wu Y,Sugimoto N,Asakura M,Nishimura K,Yamasaki S

更新日期：2006-11-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg...

journal_title：Journal of clinical microbiology

authors： Tsai TF,Bolin RA,Montoya M,Bailey RE,Francy DB,Jozan M,Roehrig JT

更新日期：1987-02-01 00:00:00

abstract：:A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid S...

journal_title：Journal of clinical microbiology

authors： Teti G,Burdash NM,Zamboni C,Fava C,Tomasello F,Mastroeni P

更新日期：1984-12-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:Many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. DiversiLab (DL; bioMérieux) potentially overcomes these limitations. In this study, we evaluated the DL system for the identification of hospital outbreaks of a number bacterial species. Appropriately typed clinica...

journal_title：Journal of clinical microbiology

authors： Fluit AC,Terlingen AM,Andriessen L,Ikawaty R,van Mansfeld R,Top J,Cohen Stuart JW,Leverstein-van Hall MA,Boel CH

更新日期：2010-11-01 00:00:00

abstract：:A neutralizing monoclonal antibody, B37:1, against a human rotavirus (B37) with a "super-short" RNA pattern was derived. The antibody gave serotype-specific reactions with a panel of standard rotaviruses and was reactive in an enzyme immunoassay. Epidemiological studies of this new human serotype should now be possibl...

journal_title：Journal of clinical microbiology

authors： Tursi JM,Albert MJ,Bishop RF

更新日期：1987-12-01 00:00:00

abstract：:Enzyme-linked immunosorbent assays were developed for the quantitation of anti-reovirus immunoglobulin G (IgG), IgA, and IgM in serum. The assays were specific, sensitive, and reproducible and measured antibodies to the reovirus group antigen. They should permit diagnosis of recent reovirus infection and longitudinal ...

journal_title：Journal of clinical microbiology

authors： Richardson SC,Bishop RF,Smith AL

更新日期：1988-09-01 00:00:00

abstract：:Forty human clinical Mycobacterium avium-M. intracellulare complex strains isolated in Greece were characterized to the species level by PCR with three sets of primers specific for one or both species. M. avium predominated in both human immunodeficiency virus-positive and -negative patients, but the frequency of M. i...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulos AM,Tassios PT,Matsiota-Bernard P,Marinis E,Tsaousidou S,Legakis NJ

更新日期：1997-11-01 00:00:00

abstract：:Random urine samples from hospitalized patients (n = 550) and seeded sterile filtered urine samples (n = 730) were used to test a membrane filtration technique, Qualture (Future Medical Technologies International, Inc., West Palm Beach, Fla.), for the detection and identification of uropathogens. Results for each samp...

journal_title：Journal of clinical microbiology

authors： Friedman MP,Danielski JM,Day TE,Dunne JC,Evangelista AT,Freeman TR

更新日期：1991-11-01 00:00:00

abstract：:Current methods for the diagnosis of melioidosis are based on bacteriological culture. A number of serological tests currently available lack specificity and sensitivity. This is largely due to the use of crude antigens which results in a significant cross-reactivity with sera from individuals infected with other bact...

journal_title：Journal of clinical microbiology

authors： Anuntagool N,Rugdech P,Sirisinha S

更新日期：1993-05-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:During a taxonomic investigation of Nocardia spp. and Streptomyces spp., we received 658 isolates from laboratories of both human and veterinary medicine. Our procedure leading to the identification of 92% of these isolates, the species that they represented, and a characteristic pattern of properties of the strains o...

journal_title：Journal of clinical microbiology

authors： Mishra SK,Gordon RE,Barnett DA

更新日期：1980-06-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...

journal_title：Journal of clinical microbiology

authors： Haque NZ,Arshad S,Peyrani P,Ford KD,Perri MB,Jacobsen G,Reyes K,Scerpella EG,Ramirez JA,Zervos MJ

更新日期：2012-05-01 00:00:00