Detection of St. Louis encephalitis virus antigen in mosquitoes by capture enzyme immunoassay.


:Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg/0.1 ml when antigen was incubated overnight; 250 pg/0.1 ml was detected in a single-day assay (antigen incubated for 3 h). The assay detected 67.9 and 70.8% of laboratory-prepared pools of infected mosquitoes after 3 h and overnight incubation, respectively. The sensitivity of the procedure was 90.5% in identifying pools with infectious titers greater than dex 3.0. The specificity of the assay was controlled by retesting positive pools preincubated with SLE virus and normal antibodies, which led to a diminution of signal in the pools containing viral antigen. The procedure was suitably specific in discriminating between SLE and related flaviviruses, detecting only high infectious doses of heterologous antigens.


J Clin Microbiol


Tsai TF,Bolin RA,Montoya M,Bailey RE,Francy DB,Jozan M,Roehrig JT




Has Abstract


1987-02-01 00:00:00












  • Development and evaluation of a rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus.

    abstract::We evaluated a new rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus by using real-time reverse transcription-PCR (rRT-PCR) and viral culture. The sensitivities were 68.5% and 64.5%, and the specificities were 98.4% and 97.6%, respectively. This kit should be used with caution, and negative ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kwon D,Shin K,Kwon M,Oh HB,Kang C,Lee JY

    更新日期:2011-01-01 00:00:00

  • Septicemia caused by Leifsonia aquatica in a healthy patient after retinal reattachment surgery.

    abstract::Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryope...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Han L,Lei JE,Wang X,Guo LT,Kang QY,He L,Xu JR

    更新日期:2013-11-01 00:00:00

  • Development and evaluation of oligonucleotide chip based on the 16S-23S rRNA gene spacer region for detection of pathogenic microorganisms associated with sepsis.

    abstract::Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kim CM,Song ES,Jang HJ,Kim HJ,Lee S,Shin JH,Kim SJ,Jeong SH,Jeong J,Koh K,Choi GE,Lee EY,Chang CL

    更新日期:2010-05-01 00:00:00

  • Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography.

    abstract::Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

    更新日期:1996-05-01 00:00:00

  • Genetic diversity among food-borne and waterborne norovirus strains causing outbreaks in Sweden.

    abstract::A total of 101 food-borne and waterborne outbreaks that were caused by norovirus and that resulted in more than 4,100 cases of illness were reported to the Swedish Institute for Infectious Disease Control from January 2002 to December 2006. Sequence and epidemiological data for isolates from 73 outbreaks were analyzed...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lysén M,Thorhagen M,Brytting M,Hjertqvist M,Andersson Y,Hedlund KO

    更新日期:2009-08-01 00:00:00

  • Reverse transcription-PCR detection of hepatitis G virus.

    abstract::Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

    更新日期:1996-11-01 00:00:00

  • Real-time quantitative PCR detection of four human bocaviruses.

    abstract::Human bocavirus (HBoV) was discovered in 2005 and is associated with respiratory tract symptoms in young children. Three additional members of the genus Bocavirus, HBoV2, -3, and -4, were discovered recently from fecal specimens, and early results indicate an association between HBoV2 and gastrointestinal disease. In ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kantola K,Sadeghi M,Antikainen J,Kirveskari J,Delwart E,Hedman K,Söderlund-Venermo M

    更新日期:2010-11-01 00:00:00

  • Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole.

    abstract::Although standard conditions are available for testing the susceptibilities of filamentous fungi to antifungal agents by the Clinical and Laboratory Standards Institute (CLSI; formerly National Committee for Clinical Laboratory Standards) broth microdilution assay, quality control (QC) MIC limits have not been establi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller M,Rinaldi M,Schell W,Walsh T

    更新日期:2005-10-01 00:00:00

  • Epidemiology of severe Streptococcus pyogenes disease in Europe.

    abstract::The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these diseases within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance of sev...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lamagni TL,Darenberg J,Luca-Harari B,Siljander T,Efstratiou A,Henriques-Normark B,Vuopio-Varkila J,Bouvet A,Creti R,Ekelund K,Koliou M,Reinert RR,Stathi A,Strakova L,Ungureanu V,Schalén C,Strep-EURO Study Group.,Jasir A

    更新日期:2008-07-01 00:00:00

  • Beijing genotype of Mycobacterium tuberculosis is significantly associated with human immunodeficiency virus infection and multidrug resistance in cases of tuberculous meningitis.

    abstract::Multidrug-resistant tuberculous meningitis is fatal without rapid diagnosis and use of second-line therapy. It is more common in human immunodeficiency virus (HIV)-positive patients. Beijing genotype strains of Mycobacterium tuberculosis are associated with drug resistance, particularly multidrug resistance, and their...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Caws M,Thwaites G,Stepniewska K,Nguyen TN,Nguyen TH,Nguyen TP,Mai NT,Phan MD,Tran HL,Tran TH,van Soolingen D,Kremer K,Nguyen VV,Nguyen TC,Farrar J

    更新日期:2006-11-01 00:00:00

  • Point-Counterpoint: Large Multiplex PCR Panels Should Be First-Line Tests for Detection of Respiratory and Intestinal Pathogens.

    abstract::The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schreckenberger PC,McAdam AJ

    更新日期:2015-10-01 00:00:00

  • Comparison of four charcoal media for the isolation of Bordetella pertussis.

    abstract::Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hoppe JE,Schwaderer J

    更新日期:1989-05-01 00:00:00

  • Incidence of and risk factors for symptomatic visceral leishmaniasis among human immunodeficiency virus type 1-infected patients from Spain in the era of highly active antiretroviral therapy.

    abstract::The way in which the extensive use of highly active antiretroviral therapy (HAART) has influenced the incidence of visceral leishmaniasis (VL) among human immunodeficiency type 1 (HIV-1)-infected patients is not yet understood. The present study assessed whether the incidence of symptomatic VL in HIV-infected patients...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: de La Rosa R,Pineda JA,Delgado J,Macías J,Morillas F,Mira JA,Sánchez-Quijano A,Leal M,Lissen E

    更新日期:2002-03-01 00:00:00

  • Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis.

    abstract::A total of 46 Borrelia burgdorferi sensu lato isolates that were isolated from patients with Lyme borreliosis and infected animals or were extracted from ticks of the genus Ixodes were analyzed. Large restriction fragment patterns obtained after cleavage of genomic DNAs with MluI were analyzed by pulsed-field gel elec...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Belfaiza J,Postic D,Bellenger E,Baranton G,Girons IS

    更新日期:1993-11-01 00:00:00

  • Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.

    abstract::A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

    更新日期:2012-03-01 00:00:00

  • Investigation of apparent false-positive urine latex particle agglutination tests for the detection of group B streptococcus antigen.

    abstract::In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Harris MC,Deuber C,Polin RA,Nachamkin I

    更新日期:1989-10-01 00:00:00

  • Evaluation of the Sceptor microdilution antibiotic susceptibility testing system: a collaborative investigation.

    abstract::A multiwell, dried antimicrobial agent susceptibility test system, Sceptor (BBL Microbiology Systems, Cockeysville, Md.), was tested. The system was compared directly with a reference microdilution method by using two collections of stock cultures and 305 fresh clinical isolates. Sceptor was found to be in agreement (...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jones RN,Thornsberry C,Barry AL,Gavan TL

    更新日期:1981-01-01 00:00:00

  • Quantitative detection of Streptococcus pneumoniae in nasopharyngeal secretions by real-time PCR.

    abstract::Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nas...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Greiner O,Day PJ,Bosshard PP,Imeri F,Altwegg M,Nadal D

    更新日期:2001-09-01 00:00:00

  • Use of pulsed-field gel electrophoresis typing to study an outbreak of infection due to Serratia marcescens in a neonatal intensive care unit.

    abstract::Serratia marcescens is a well-known cause of nosocomial infections and outbreaks, particularly in critically ill neonates and immunocompromised patients. Numerous methods have been proposed for typing. We used pulsed-field gel electrophoresis (PFGE) typing to analyze an outbreak in a neonatal intensive care unit (NICU...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Miranda G,Kelly C,Solorzano F,Leanos B,Coria R,Patterson JE

    更新日期:1996-12-01 00:00:00

  • Sequence analysis of the p1 adhesin gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2008 to 2009.

    abstract::The p1 genes of 60 Mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. An AGT trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. In addition, a novel p1 gene variant named 2c was identified in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhao F,Cao B,Li J,Song S,Tao X,Yin Y,He L,Zhang J

    更新日期:2011-08-01 00:00:00

  • Sequencing-based genotyping of mixed human papillomavirus infections by use of RipSeq software.

    abstract::Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

    更新日期:2013-04-01 00:00:00

  • Novel Single Nucleotide Polymorphism-Based Assay for Genotyping Mycobacterium avium subsp. paratuberculosis.

    abstract::Typing of Mycobacterium avium subspecies paratuberculosis strains presents a challenge, since they are genetically monomorphic and traditional molecular techniques have limited discriminatory power. The recent advances and availability of whole-genome sequencing have extended possibilities for the characterization of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Leão C,Goldstone RJ,Bryant J,McLuckie J,Inácio J,Smith DG,Stevenson K

    更新日期:2016-03-01 00:00:00

  • Routine identification of Mycobacterium tuberculosis complex isolates by automated hybridization.

    abstract::Methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. We describe here a 2-h identification system for mycobacterial isolates belonging to the Mycobacterium tuberculosis complex using a DNA probe. After 30 min of hands...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mabilat C,Desvarenne S,Panteix G,Machabert N,Bernillon MH,Guardiola G,Cros P

    更新日期:1994-11-01 00:00:00

  • Multicenter evaluation of the new Vitek 2 yeast susceptibility test using new CLSI clinical breakpoints for fluconazole.

    abstract::A fully automated antifungal susceptibility test system recently updated to reflect the new species-specific clinical breakpoints (CBPs) of fluconazole for Candida (Vitek 2 AF03 yeast susceptibility test; bioMérieux, Inc., Durham, NC) was compared in three different laboratories with the Clinical and Laboratory Standa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Pfaller MA,Diekema DJ,Procop GW,Wiederhold NP

    更新日期:2014-06-01 00:00:00

  • Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.

    abstract::We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobul...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

    更新日期:2004-05-01 00:00:00

  • Characterization of human cytomegalovirus strains by analysis of short tandem repeat polymorphisms.

    abstract::Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Walker A,Petheram SJ,Ballard L,Murph JR,Demmler GJ,Bale JF Jr

    更新日期:2001-06-01 00:00:00

  • High-resolution melting approach to efficient identification and quantification of H275Y mutant influenza H1N1/2009 virus in mixed-virus-population samples.

    abstract::The single-nucleotide variation 823C to T (His275Tyr), responsible for oseltamivir drug resistance has been detected in some isolates of the influenza A/H1N1/2009 virus. Early detection of the presence of this oseltamivir-resistant strain allows prompt consideration of alternative treatment options. An isolated-probe-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lee HK,Lee CK,Loh TP,Tang JW,Tambyah PA,Koay ES

    更新日期:2011-10-01 00:00:00

  • Exposure of laboratory workers to Francisella tularensis despite a bioterrorism procedure.

    abstract::A rapidly fatal case of pulmonary tularemia in a 43-year-old man who was transferred to a tertiary care facility is presented. The microbiology laboratory and autopsy services were not notified of the clinical suspicion of tularemia by the service caring for the patient. Despite having a laboratory bioterrorism proced...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shapiro DS,Schwartz DR

    更新日期:2002-06-01 00:00:00

  • Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus.

    abstract::A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Costas M,Cookson BD,Talsania HG,Owen RJ

    更新日期:1989-11-01 00:00:00

  • Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry.

    abstract::We propose a novel application of secondary electrospray ionization-mass spectrometry (SESI-MS) as a real-time clinical diagnostic tool for bacterial infection. It is known that volatile organic compounds (VOCs), produced in different combinations and quantities by bacteria as metabolites, generate characteristic odor...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhu J,Bean HD,Kuo YM,Hill JE

    更新日期:2010-12-01 00:00:00