Laboratory experience with a radiometric method for detecting bacteremia.

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

journal_title：Journal of clinical microbiology

authors： Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

更新日期：1999-12-01 00:00:00

abstract：:The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR. ...

journal_title：Journal of clinical microbiology

authors： Aichinger E,Schleck CD,Harmsen WS,Nyre LM,Patel R

更新日期：2008-11-01 00:00:00

abstract：:This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

journal_title：Journal of clinical microbiology

authors： Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

更新日期：2014-12-01 00:00:00

abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

journal_title：Journal of clinical microbiology

authors： Kerremans JJ,Verbrugh HA,Vos MC

更新日期：2012-06-01 00:00:00

abstract：:Human bocavirus (HBoV) was discovered in 2005 and is associated with respiratory tract symptoms in young children. Three additional members of the genus Bocavirus, HBoV2, -3, and -4, were discovered recently from fecal specimens, and early results indicate an association between HBoV2 and gastrointestinal disease. In ...

journal_title：Journal of clinical microbiology

authors： Kantola K,Sadeghi M,Antikainen J,Kirveskari J,Delwart E,Hedman K,Söderlund-Venermo M

更新日期：2010-11-01 00:00:00

abstract：:Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

journal_title：Journal of clinical microbiology

authors： Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

更新日期：2003-06-01 00:00:00

abstract：:Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

journal_title：Journal of clinical microbiology

authors： Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

更新日期：2014-10-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, ...

journal_title：Journal of clinical microbiology

authors： Widjaja S,Cohen S,Brady WE,O'reilly K,Susanto,Wibowo A,Cahyono,Graham RR,Porter KR

更新日期：1999-12-01 00:00:00

abstract：:Rhodotorula infections occur among patients with immunosuppression and/or central venous catheters. Using standardized methods (NCCLS M27-A), we determined the antifungal susceptibilities of 10 Rhodotorula bloodstream infection isolates. Patient information was collected for clinical correlation. The MICs of amphoteri...

journal_title：Journal of clinical microbiology

authors： Zaas AK,Boyce M,Schell W,Lodge BA,Miller JL,Perfect JR

更新日期：2003-11-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inac...

journal_title：Journal of clinical microbiology

authors： Christianson GG,Pemberton JR,Koski TA

更新日期：1980-04-01 00:00:00

abstract：:A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...

journal_title：Journal of clinical microbiology

authors： Dhar AK,Roux MM,Klimpel KR

更新日期：2001-08-01 00:00:00

abstract：:Crude extracts of Dolichos biflorus can be coupled to polystyrene particles to yield an agglutination reagent for the detection of group C streptococcal antigen extracts. The reagent is relatively inexpensive and simple to prepare and can be employed for the definitive identification of this beta-hemolytic streptococc...

journal_title：Journal of clinical microbiology

authors： Slifkin M,Gil GM

更新日期：1984-01-01 00:00:00

abstract：:Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) re...

journal_title：Journal of clinical microbiology

authors： Zengzhu G,Bracha R,Nuchamowitz Y,Cheng-I W,Mirelman D

更新日期：1999-09-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:We describe the first case of Epstein-Barr virus (EBV)-associated thymic carcinoid tumor found by in situ hybridization (ISH) on paraffin-embedded sections. ISH revealed that both tumor cells and infiltrated lymphocytes were EBV positive, while a few EBV-infected lymphocytes were detected in 2 of 11 thymuses and 1 of ...

journal_title：Journal of clinical microbiology

authors： Takeuchi H,Fujita H,Iwasaki F,Takeuchi T,Imadome K,Okumiya T,Matsui T,Takahashi M

更新日期：2004-06-01 00:00:00

abstract：:Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...

journal_title：Journal of clinical microbiology

authors： Kurosaki Y,Grolla A,Fukuma A,Feldmann H,Yasuda J

更新日期：2010-07-01 00:00:00

abstract：:In a prospective study, 139 serial blood samples from 15 transplant recipients were assessed for the presence of cytomegalovirus (CMV) by virus isolation (CMV viremia) and by direct staining of CMV antigens (CMV Ag) in blood leukocytes (CMV antigenemia). CMV was isolated from 23 samples, whereas CMV Ag was detected in...

journal_title：Journal of clinical microbiology

authors： van der Bij W,Schirm J,Torensma R,van Son WJ,Tegzess AM,The TH

更新日期：1988-12-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

更新日期：1986-11-01 00:00:00

abstract：:During 1984, the recovery of enteric pathogens from patients with acute diarrhea was enhanced by the use of both rectal swab and stool specimens. With 513 patients for whom both methods were used, the overall recovery rate was increased a minimum of about 10%. Almost 50% of the organisms recovered were detected by onl...

journal_title：Journal of clinical microbiology

authors： Adkins HJ,Santiago LT

更新日期：1987-01-01 00:00:00

abstract：:Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...

journal_title：Journal of clinical microbiology

authors： Effelsberg N,Stegger M,Peitzmann L,Altinok O,Coombs GW,Pichon B,Kearns A,Randad PR,Heaney CD,Bletz S,Schaumburg F,Mellmann A

更新日期：2020-12-17 00:00:00

abstract：:Of a total of 1043 macrolide-resistant Streptococcus pneumoniae isolates collected from 24 countries as part of PROTEKT 1999-2000, 71 isolates tested positive for both the mef(A) and erm(B) genes. Of 69 isolates subjected to further molecular investigations, all were resistant to tetracycline, 63 (91.3%) were resistan...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Morrissey I,Bakker S,Morris L,Buckridge S,Felmingham D

更新日期：2004-02-01 00:00:00

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:Scedosporium prolificans is a soil saprophyte that is associated with a large variety of infectious processes and with respiratory colonization in immunocompetent and immunocompromised patients. We report the first described case of S. prolificans keratouveitis associated with the intraocular long-term retention of a ...

journal_title：Journal of clinical microbiology

authors： Arthur S,Steed LL,Apple DJ,Peng Q,Howard G,Escobar-Gomez M

更新日期：2001-12-01 00:00:00

abstract：:The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...

journal_title：Journal of clinical microbiology

authors： Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze A

更新日期：2006-10-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00