Abstract:
:The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA profiles. In contrast, pulsed-field gel electrophoresis (PFGE) identified 20 distinct PFGE types among the 52 strains examined, which indicates that PFGE is more discriminating than is 16S rRNA sequence analysis for differentiating S. aureus strains. The results of our PFGE typing also suggest that multiple genetic subpopulations (related at the ca. 85% similarity level, based on their SmaI PFGE patterns) exist among the Georgian S. aureus strains. Twenty-two of the 50 Georgian strains were methicillin resistant and PCR positive for mecA, and 5 strains were methicillin sensitive even though they possessed mecA. None of the strains were vancomycin resistant or contained vanA. The nucleotide sequences of mecA fragments obtained from all mecA-containing strains were identical. Our data indicate that the population of S. aureus strains in Georgia is fairly homogeneous and that the prevalence of methicillin-resistant, mecA-positive strains is relatively high in that country.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze Adoi
10.1128/JCM.01030-06subject
Has Abstractpub_date
2006-10-01 00:00:00pages
3477-83issue
10eissn
0095-1137issn
1098-660Xpii
44/10/3477journal_volume
44pub_type
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