Identification of group C streptococcal antigen extracts with lectin-bound polystyrene particles.

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:A standardized broth microdilution method was compared to the E test and an agar dilution method for the antimicrobial susceptibility testing of Campylobacter jejuni and C. coli isolates. A group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) w...

journal_title：Journal of clinical microbiology

authors： Luber P,Bartelt E,Genschow E,Wagner J,Hahn H

更新日期：2003-03-01 00:00:00

abstract：:Myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. The spores were identical to those of Myxobolus plectroplites, a previously described pathogen from the freshwater fish Plectroplites ambiguus. All patients had recently eaten fish caught from local water...

journal_title：Journal of clinical microbiology

authors： Boreham RE,Hendrick S,O'Donoghue PJ,Stenzel DJ

更新日期：1998-12-01 00:00:00

abstract：:A laboratory-based surveillance network of 11 clinical virological laboratories for influenza viruses was established in Taiwan under the coordination of the Center for Disease Control and Prevention (CDC), Taiwan. From October 2000 to March 2004, 3,244 influenza viruses were isolated, including 1,969 influenza A and ...

journal_title：Journal of clinical microbiology

authors： Shih SR,Chen GW,Yang CC,Yang WZ,Liu DP,Lin JH,Chiu SC,Chen HY,Tsao KC,Huang CG,Huang YL,Mok CK,Chen CJ,Lin TY,Wang JR,Kao CL,Lin KH,Chen LK,Eng HL,Liu YC,Chen PY,Lin JS,Wang JH,Lin CW,Chan YJ,Lu JJ,Hsi

更新日期：2005-04-01 00:00:00

abstract：:Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

journal_title：Journal of clinical microbiology

authors： Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

更新日期：2006-09-01 00:00:00

abstract：:Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. dubliniensis are susceptible to established antifungal agents, fluconazole-resistant strains have been detected. Furthermore, fluconazole-resistant strains ...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Messer SA,Gee S,Joly S,Pujol C,Sullivan DJ,Coleman DC,Soll DR

更新日期：1999-03-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

journal_title：Journal of clinical microbiology

authors： Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

更新日期：1986-08-01 00:00:00

abstract：:Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...

journal_title：Journal of clinical microbiology

authors： Mellado E,Diaz-Guerra TM,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2001-07-01 00:00:00

abstract：:A new disposable plastic tube device and technique for ovum and parasite concentration, with upper and lower chambers separated by a fine screen, was compared with the standard gauze and open-funnel filtration technique as well as with the technique of direct smearing with an acid-fast stain. The new concentrator was ...

journal_title：Journal of clinical microbiology

authors： Zierdt WS

更新日期：1984-11-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:A total of 28 unrelated isolates of the Salmonella enterica subsp. enterica serovar dublin (S. dublin) collected during a 6-year period, as well as four samples of the S. dublin live vaccine strain Bovisaloral and its prototype strain S. dublin 442/039, were investigated by different molecular typing methods for the f...

journal_title：Journal of clinical microbiology

authors： Liebisch B,Schwarz S

更新日期：1996-03-01 00:00:00

abstract：:The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA). Consecutive urine specimens from 101 pregnant women and 287 nonpreg...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Jang D,Luinstra K,Faught M,Dalby D,Sellors J,Chernesky M

更新日期：1998-11-01 00:00:00

abstract：:Because of its low yield in unselected specimens, stool culture is often cost ineffective. We tested 55 fecal samples from Fairfax Hospital (46 patients with diarrhea and 9 from controls without diarrhea) for lactoferrin by latex agglutination (LFLA) with the Leukotest (Techlab, Blacksburg, Va.) as a marker for inflam...

journal_title：Journal of clinical microbiology

authors： Choi SW,Park CH,Silva TM,Zaenker EI,Guerrant RL

更新日期：1996-04-01 00:00:00

abstract：:Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

journal_title：Journal of clinical microbiology

authors： van Belkum A,Struelens M,Quint W

更新日期：1993-08-01 00:00:00

abstract：:One of the major unanswered questions regarding the presence of Listeria monocytogenes in foods is how many cells must be ingested in order to cause illness. To answer this question, studies were undertaken by using Macaca fascicularis (cynomolgus monkey) as an animal model. Healthy nonhuman primates were dosed with v...

journal_title：Journal of clinical microbiology

authors： Farber JM,Daley E,Coates F,Beausoleil N,Fournier J

更新日期：1991-11-01 00:00:00

abstract：:A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agg...

journal_title：Journal of clinical microbiology

authors： Smits HL,van der Hoorn MA,Goris MG,Gussenhoven GC,Yersin C,Sasaki DM,Terpstra WJ,Hartskeerl RA

更新日期：2000-03-01 00:00:00

abstract：:The arylsulfatase isozymes of Mycobacterium fortuitum, M. peregrinum, M. chelonei subsp. chelonei, and M. chelonei subsp. abscessus were examined to determine the isozymal and immunological relationship among the members of the M. fortuitum complex. Cell extracts were subjected to electrophoresis on agarose and polyac...

journal_title：Journal of clinical microbiology

authors： Binko BP,Bartholomew WR

更新日期：1978-08-01 00:00:00

abstract：:This study describes the molecular identification of 520 Entamoeba-positive fecal samples from a large and diverse population of captive nonhuman primates (NHP). The results revealed the presence of Entamoeba histolytica (NHP variant only), E. dispar, E. moshkovskii, E. hartmanni, E. coli, and E. polecki-like organism...

journal_title：Journal of clinical microbiology

authors： Levecke B,Dreesen L,Dorny P,Verweij JJ,Vercammen F,Casaert S,Vercruysse J,Geldhof P

更新日期：2010-08-01 00:00:00

abstract：:A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analys...

journal_title：Journal of clinical microbiology

authors： Adékambi T,Stein A,Carvajal J,Raoult D,Drancourt M

更新日期：2006-04-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:Four serotypes of Cryptococcus neoformans designated A, B, C, and D are currently recognized. Although an agglutination test is most often used to serotype C. neoformans in cultures, this test is not appropriate for typing the fungus in fixed tissues. A study to prepare fluorescent-antibody reagents for typing C. neof...

journal_title：Journal of clinical microbiology

authors： Kaplan W,Bragg SL,Crane S,Ahearn DG

更新日期：1981-09-01 00:00:00

abstract：:The peripheral blood lymphocytes from 48 heparinized blood specimens from human immunodeficiency virus (HIV) antibody-positive individuals were divided into two aliquots. One aliquot was reconstituted in one of the following five media. Medium 1 consisted of tryptose broth with 0.5% gelatin; medium 2 consisted of RPMI...

journal_title：Journal of clinical microbiology

authors： Gallo D,Kimpton JS,Johnson PJ

更新日期：1989-01-01 00:00:00

abstract：:Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to Oc...

journal_title：Journal of clinical microbiology

authors： Chatzigeorgiou KS,Sergentanis TN,Tsiodras S,Hamodrakas SJ,Bagos PG

更新日期：2011-09-01 00:00:00

abstract：:psaA is the gene encoding pneumococcal surface adhesin A (PsaA), a 37-kDa protein expressed on the surface of Streptococcus pneumoniae. PCR primers for psaA have been shown to amplify the target DNA sequence in all 90 serotypes of S. pneumoniae and in none of 67 heterologous pathogens and colonizing bacteria of the up...

journal_title：Journal of clinical microbiology

authors： Scott JA,Marston EL,Hall AJ,Marsh K

更新日期：2003-06-01 00:00:00

abstract：:The findings from a preliminary assessment of a new instrument designed for the inoculation and spreading of specimens for microbiological analysis onto agar plates are described. The study found that the instrument was able to select full or biplates from a number of input cassettes, each containing different agar ty...

journal_title：Journal of clinical microbiology

authors： Glasson JH,Guthrie LH,Nielsen DJ,Bethell FA

更新日期：2008-04-01 00:00:00

abstract：:A selective medium was developed for the isolation of Bacteroides fragilis directly from ovine and bovine fecal samples. The medium (tryptose blood agar plus polymyxin B, triclosan (Irgasan), novobiocin, and nalidixic acid) permitted growth of B. fragilis and several other species of Bacteroides but inhibited growth o...

journal_title：Journal of clinical microbiology

authors： Border M,Firehammer BD,Shoop DS,Myers LL

更新日期：1985-03-01 00:00:00

abstract：:A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

journal_title：Journal of clinical microbiology

authors： Beaucage CM,Onderdonk AB

更新日期：1982-09-01 00:00:00