Abstract:
:In the SIGNAL (Oxoid Ltd., Basingstoke, United Kingdom) blood culture system, gas produced by bacterial metabolism displaces medium from the culture bottle into an upper reservoir via a hollow needle. Displacement of media may provide a visual indication of the presence of both aerobic and anaerobic organisms in a single medium. The single-bottle SIGNAL system was compared with paired BACTEC 16B and 7D (Johnston Laboratories, Inc., Towson, Md.) radiometric system bottles by using bacterial inocula and conditions which simulated those found in neonatal and pediatric populations. The single SIGNAL bottle was a good as the combined BACTEC media for Escherichia coli and Staphylococcus aureus, but was slower for Candida spp., Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus epidermidis, group B streptococci, alpha-streptococci, and pneumococci. The SIGNAL system failed to detect four of five isolates of Neisseria meningitidis and four of eight anaerobic organisms. The SIGNAL system is not suitable for neonatal blood cultures at its present state of development.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Trombley C,Anderson JDdoi
10.1128/JCM.25.11.2098-2101.1987subject
Has Abstractpub_date
1987-11-01 00:00:00pages
2098-101issue
11eissn
0095-1137issn
1098-660Xjournal_volume
25pub_type
杂志文章abstract::Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.10.5327-5331.2005
更新日期:2005-10-01 00:00:00
abstract::To assess whether rinsing with oral antiseptics before sputum collection would reduce contamination of mycobacterial cultures, 120 patients with suspected tuberculosis were randomly assigned to rinse with chlorhexidine or cetylpyridinium mouthwash before collection. The culture contamination rate was significantly low...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,随机对照试验
doi:10.1128/JCM.00541-11
更新日期:2011-08-01 00:00:00
abstract::Serologic methods are an important part of a clinical laboratory's portfolio of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests and are essential to the broader response to coronavirus infectious disease 2019 (COVID-19), including epidemiological studies and vaccine development. There are currently ...
journal_title:Journal of clinical microbiology
pub_type: 评论,杂志文章
doi:10.1128/JCM.02179-20
更新日期:2020-10-21 00:00:00
abstract::Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01483-12
更新日期:2012-11-01 00:00:00
abstract::In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.44.1.67-70.2006
更新日期:2006-01-01 00:00:00
abstract::We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.12.6200-6201.2005
更新日期:2005-12-01 00:00:00
abstract::A case of septicemia caused by Pseudomonas stutzeri belonging to the unusual biotype Vb-3 in a patient with multiple myeloma is described. The origin of the septicemia was attributed to a community-acquired pneumonia. The bacteriology and pathogenicity of P. stutzeri are reviewed. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.2.458-459.1987
更新日期:1987-02-01 00:00:00
abstract::Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. We evaluated two multiplex reverse transcription-PCR (RT-PCR) products coupled with microarray-based systems ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00210-07
更新日期:2007-07-01 00:00:00
abstract::A case of zygomycosis caused by Apophysomyces elegans in a patient having 25% full-thickness burns is described. Amputation of the leg was necessary to control rapid tissue invasion. The fungus was isolated from soil in the burn environment. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.9.2151-2153.1990
更新日期:1990-09-01 00:00:00
abstract::Urinary tract infections caused by staphylococci are usually attributed to Staphylococcus epidermidis or S. saprophyticus. The case study reported here describes a persistent urinary tract infection caused by S. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibioti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.5.1055-1057.1988
更新日期:1988-05-01 00:00:00
abstract::We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.2.660-662.2002
更新日期:2002-02-01 00:00:00
abstract::PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00770-09
更新日期:2009-08-01 00:00:00
abstract::A batch of fetal calf serum was encountered which suppressed the number of Chlamydia trachomatis inclusions seen when it was used in conjunction with cycloheximide-treated McCoy cells. The batch of serum did not affect the number of inclusions detected in irradiated or untreated McCoy cells. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.11.4.424-425.1980
更新日期:1980-04-01 00:00:00
abstract::An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.2.323-327.1991
更新日期:1991-02-01 00:00:00
abstract::The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens of Treponema pallidum. Pronounced cross-reactivities with other spirochete antigens are often reported. The aim of this study was to validate a novel multiparametric assay (the assay performed with the kit) INNO-...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Identification of members of the Mycobacterium tuberculosis complex and the M. avium-M. intracellulare complex (MAC) directly from primary BACTEC cultures was evaluated by using acridinium-ester-labeled DNA probes (AccuProbe; GenProbe, Inc., San Diego, Calif.). In preliminary experiments, blood present in samples was ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.9.2427-2431.1992
更新日期:1992-09-01 00:00:00
abstract::We evaluated three rapid identification systems-The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems-for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigran...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::We report on a case of conjunctivitis caused by a nutritionally variant streptococcus in a newborn infant. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.2.379-380.1986
更新日期:1986-02-01 00:00:00
abstract::The results of anaerobic susceptibility tests performed with inocula prepared directly from agar isolation media and from overnight broth cultures were compared. Altogether, 93.0% of the results with these two inoculum preparations were within one twofold dilution, including 92.5 and 95.6% of the results with Clostrid...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.3.733-734.1983
更新日期:1983-09-01 00:00:00
abstract::The pathogenicity of the fungus Geotrichum candidum in humans has not been clearly defined. A patient with acute leukemia who developed a fatal disseminated G. candidum infection while neutropenic is described. At autopsy examination, this patient was misdiagnosed on the basis of histopathology as having disseminated ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.9.1782-1783.1987
更新日期:1987-09-01 00:00:00
abstract::Group B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding anti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00355-16
更新日期:2016-07-01 00:00:00
abstract::Three cloned segments of Mycobacterium tuberculosis DNA which are promising as clinical probes were identified. An MboI digest of DNA from a clinical isolate of M. tuberculosis was cloned into bacteriophage M13. To identify recombinants specific for the M. tuberculosis complex, plaque lifts were hybridized with M. bov...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.11.2240-2245.1988
更新日期:1988-11-01 00:00:00
abstract::Screening of blood for human T-cell lymphotropic virus type 1 and type 2 (HTLV-1 and -2, respectively) is important to diagnose and prevent infection and ensure the safety of blood supplies. The Elecsys HTLV-I/II assay is a newly developed, electrochemiluminescence screening assay for the detection of HTLV-1/2 infecti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00169-17
更新日期:2017-07-01 00:00:00
abstract::In the context of hematopoietic stem cell transplantation, adenovirus infections are associated with relevant mortality and morbidity. Detection of adenovirus DNA by quantitative PCR is the "gold standard" for these patients. A total of 150 samples, namely, 78 whole-blood, 22 cerebrospinal fluid, 24 digestive biopsy, ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00976-10
更新日期:2010-09-01 00:00:00
abstract::Autopsy specimens of lung tissues from 15 patients that contracted legionellosis during the 1976 Philadelphia outbreak of Legionnaires disease were examined for the presence of Legionella organisms and soluble antigens by indirect fluorescent-antibody (IFA) testing and by an enzyme-linked immunosorbent assay (ELISA) w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.21.1.15-19.1985
更新日期:1985-01-01 00:00:00
abstract::Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Genotyping studies suggest that genetic variability exists among P. gingivalis strains; however, the extent of variability remains unclear and regions of variability remain largely unidentified. To assess P. gingivalis strain diversity, w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00569-09
更新日期:2009-10-01 00:00:00
abstract::We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high sp...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.1.35-38.1999
更新日期:1999-01-01 00:00:00
abstract::Patients with atrophic corpus gastritis and elevated Helicobacter pylori antibody titers but 13C-urea breath test (13C-UBT) and histology results negative for H. pylori were randomized into eradication therapy or follow-up only. Antibody levels decreased significantly in six out of seven patients in the eradication gr...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/JCM.36.6.1808-1810.1998
更新日期:1998-06-01 00:00:00
abstract::Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.9.1867-1868.1988
更新日期:1988-09-01 00:00:00