Evaluation of oral antiseptic rinsing before sputum collection to reduce contamination of mycobacterial cultures.

abstract：:A point prevalence culture survey was carried out to investigate the prevalence of fecal carriage of vancomycin-resistant enterococci (VRE) among patients admitted to an 800-bed general hospital where no VRE had been isolated previously. Twenty-two of 636 patients (3.5%) were found to be VRE carriers. Eighteen strains...

journal_title：Journal of clinical microbiology

authors： Gordts B,Van Landuyt H,Ieven M,Vandamme P,Goossens H

更新日期：1995-11-01 00:00:00

abstract：:Enterococcus cecorum (formerly Streptococcus cecorum), originally isolated from poultry intestines, has rarely been encountered in human diseases. A 60-year-old man with liver cirrhosis and hepatocellular carcinoma developed peritonitis on the seventh day of his hospitalization. Cultures of one blood sample and one as...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Chen YC,Yang PC,Ho SW,Luh KT

更新日期：2000-06-01 00:00:00

abstract：:Using data from an ongoing Swedish intervention project, the observed durations of nasopharyngeal carriage of penicillin-nonsusceptible Streptococcus pneumoniae (PNSP) (MIC of penicillin G of >or=0.5 microg/ml) stratified by both pneumococcal serogroup and age of the carrier were compared. The means and 95% confidence...

journal_title：Journal of clinical microbiology

authors： Högberg L,Geli P,Ringberg H,Melander E,Lipsitch M,Ekdahl K

更新日期：2007-03-01 00:00:00

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomallei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively....

journal_title：Journal of clinical microbiology

authors： O'Brien M,Freeman K,Lum G,Cheng AC,Jacups SP,Currie BJ

更新日期：2004-05-01 00:00:00

abstract：:A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11 pulsed-field gel electrophoresis types; 17 contained the prototype vanB2 and 8 contained a novel vanB2 with a 177-bp deleti...

journal_title：Journal of clinical microbiology

authors： Lee WG,Jernigan JA,Rasheed JK,Anderson GJ,Tenover FC

更新日期：2001-03-01 00:00:00

abstract：:Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Was...

journal_title：Journal of clinical microbiology

authors： Nalla AK,Casto AM,Huang MW,Perchetti GA,Sampoleo R,Shrestha L,Wei Y,Zhu H,Jerome KR,Greninger AL

更新日期：2020-05-26 00:00:00

abstract：:An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the result...

journal_title：Journal of clinical microbiology

authors： Arcavi M,Orfus G,Griemberg G

更新日期：1997-06-01 00:00:00

abstract：:A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoassay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19. I...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Saint-Antoine P,Olivier C,Voyer H,Kessous-Elbaz A,Berrada F,Bégin P,Giroux L,Viscidi R

更新日期：1991-11-01 00:00:00

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

journal_title：Journal of clinical microbiology

authors： Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

更新日期：2007-06-01 00:00:00

abstract：:Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

更新日期：2009-10-01 00:00:00

abstract：:A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

journal_title：Journal of clinical microbiology

authors： Hamilton AJ,Goodley J

更新日期：1993-02-01 00:00:00

abstract：:To halt the human immunodeficiency virus type 1 (HIV-1) epidemic requires interventions that can prevent transmission of numerous HIV-1 subtypes. The most frequently transmitted viruses belong to the subtypes A, B, and C and the circulating recombinant forms (CRFs) AE and AG. A fast one-tube assay that identifies and ...

journal_title：Journal of clinical microbiology

authors： de Baar MP,Timmermans EC,Bakker M,de Rooij E,van Gemen B,Goudsmit J

更新日期：2001-05-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:We report the first case of disseminated infection with both Actinomyces graevenitzii and Mycobacterium tuberculosis and review the medical literature. Concomitant actinomycosis and tuberculosis is very rare. The potential of the facultatively aerobic, newly described A. graevenitzii for disseminated invasive infectio...

journal_title：Journal of clinical microbiology

authors： Tietz A,Aldridge KE,Figueroa JE

更新日期：2005-06-01 00:00:00

abstract：:An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

journal_title：Journal of clinical microbiology

authors： Goldwater PN

更新日期：1995-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

journal_title：Journal of clinical microbiology

authors： Fridriksdóttir V,Nesse LL,Gudding R

更新日期：1992-05-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:Accurate identification of pathogenic species is important for early appropriate patient management, but growing diversity of infectious species/strains makes the identification of clinical yeasts increasingly difficult. Among conventional methods that are commercially available, the API ID32C, AuxaColor, and Vitek 2 ...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Efremov L,Leoncini E,Amore R,Posteraro P,Ricciardi W,Sanguinetti M

更新日期：2015-08-01 00:00:00

abstract：:A novel PCR restriction analysis method using the RNA polymerase beta-subunit- coding gene (rpoB) was employed to both detect and identify Helicobacter pylori in biopsy specimens and culture isolates. The rpoB DNAs (458 bp) were specifically amplified by PCR with the Helicobacter-specific primers (HF and HR). Based on...

journal_title：Journal of clinical microbiology

authors： Lim CY,Lee KH,Cho MJ,Chang MW,Kim SY,Myong NH,Lee WK,Rhee KH,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:The number of reports concerning Escherichia coli clinical isolates that produce glutathione S-transferases responsible for fosfomycin resistance (FR-GSTs) has been increasing. We have developed a disk-based potentiation test in which FR-GST producers expand the growth inhibition zone around a Kirby-Bauer disk contain...

journal_title：Journal of clinical microbiology

authors： Nakamura G,Wachino J,Sato N,Kimura K,Yamada K,Jin W,Shibayama K,Yagi T,Kawamura K,Arakawa Y

更新日期：2014-09-01 00:00:00

abstract：:VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C(6)) with the IR(6) sequence was explored. Sensitivity was a...

journal_title：Journal of clinical microbiology

authors： Liang FT,Steere AC,Marques AR,Johnson BJ,Miller JN,Philipp MT

更新日期：1999-12-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:The identification of Pasteurella and related bacteria remains a challenge. Here, a 449- to 473-bp fragment (sodA(int)) internal to the sodA gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of Pasteurella (18 strains...

journal_title：Journal of clinical microbiology

authors： Gautier AL,Dubois D,Escande F,Avril JL,Trieu-Cuot P,Gaillot O

更新日期：2005-05-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:A total of 242 isolates were recovered from 76 patients with invasive diseases, 89 with scarlet fever, and 77 with pharyngitis. The most frequent emm types were types 12 (43.4%), 4 (18.2%), and 1 (16.9%). emm12 reemerged in 2005 and peaked in 2007. emm11 was recovered only from patients with invasive disease. ...

journal_title：Journal of clinical microbiology

authors： Su YF,Wang SM,Lin YL,Chuang WJ,Lin YS,Wu JJ,Lin MT,Liu CC

更新日期：2009-08-01 00:00:00

abstract：:We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture inf...

journal_title：Journal of clinical microbiology

authors： Aguilar JC,Pérez-Breña MP,García ML,Cruz N,Erdman DD,Echevarría JE

更新日期：2000-03-01 00:00:00

abstract：:Helicobacter pylori virulence determinants have not previously been studied in detail in Latin Americans with H. pylori infections. We characterized the vacA (vacuolating cytotoxin gene A) and cagA (cytotoxin-associated gene A) types of more than 400 single-colony isolates from 20 patients in Mexico City. For 17 patie...

journal_title：Journal of clinical microbiology

authors： Morales-Espinosa R,Castillo-Rojas G,Gonzalez-Valencia G,Ponce de León S,Cravioto A,Atherton JC,López-Vidal Y

更新日期：1999-09-01 00:00:00