Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity.

abstract：:We report a study of 1,953 patients whose laboratory records from 1972 through 1983 at the Massachusetts Mycobacteria Reference Laboratory indicated the isolation of Mycobacterium avium complex (MAC) organisms. At least one clinical specimen from each patient during this period exhibited the organism. The incidence of...

journal_title：Journal of clinical microbiology

authors： du Moulin GC,Sherman IH,Hoaglin DC,Stottmeier KD

更新日期：1985-07-01 00:00:00

abstract：:An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

journal_title：Journal of clinical microbiology

authors： Goldwater PN

更新日期：1995-06-01 00:00:00

abstract：:The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing hu...

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Jones WL

更新日期：1976-05-01 00:00:00

abstract：:The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confoun...

journal_title：Journal of clinical microbiology

authors： Silva CP,Pereira-Lima JC,Oliveira AG,Guerra JB,Marques DL,Sarmanho L,Cabral MM,Queiroz DM

更新日期：2003-12-01 00:00:00

abstract：:A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

journal_title：Journal of clinical microbiology

authors： Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

更新日期：1996-06-01 00:00:00

abstract：:Clinical evidence for failure with beta-lactam therapy has been lacking for patients with borderline oxacillin-resistant Staphylococcus aureus (BORSA) infections. We describe a failure of cloxacillin for a patient with endocarditis due to BORSA. The isolate also had false-negative thermonuclease and coagulase test res...

journal_title：Journal of clinical microbiology

authors： Skinner S,Murray M,Walus T,Karlowsky JA

更新日期：2009-03-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:An estimated 41,200 people were newly infected with hepatitis C virus (HCV) in 2016 in the United States. Screening tests for antibodies to HCV may generate up to 32% false positivity in low-risk populations. Current Centers for Disease Control and Prevention (CDC) screening recommendations do not require confirmatory...

journal_title：Journal of clinical microbiology

authors： Kodani M,Martin M,de Castro VL,Drobeniuc J,Kamili S

更新日期：2019-02-27 00:00:00

abstract：:Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

journal_title：Journal of clinical microbiology

authors： Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

更新日期：1991-09-01 00:00:00

abstract：:High incidence rates of isoniazid-, rifampin-, and multiple-drug-resistant tuberculosis have been reported in countries of the former Soviet Union (FSU). Genotypic (unlike phenotypic) drug resistance assays do not require viable cultures but require accurate knowledge of both the target gene and the mutations associat...

journal_title：Journal of clinical microbiology

authors： Nikolayevsky V,Brown T,Balabanova Y,Ruddy M,Fedorin I,Drobniewski F

更新日期：2004-10-01 00:00:00

abstract：:During 2012, we identified sampled dogs with elevated levels of antibodies (≥1:40) against A(H1N1)pdm09 virus by using a hemagglutination inhibition (HI) assay (seroprevalence, 24.7%) and a microneutralization (MN) assay (seroprevalence, 10.8%). These high seroprevalences of A(H1N1)pdm09 among dogs without clinical si...

journal_title：Journal of clinical microbiology

authors： Su S,Chen J,Jia K,Khan SU,He S,Fu X,Hong M,Sun L,Qi W,Gray GC,Li S

更新日期：2014-05-01 00:00:00

abstract：:The Limulus amoebocyte lysate (LAL) test was evaluated for rapid detection of gram-negative bacteriuria in an adult patient population. Time to gelation of a standard LAL preparation was used as a measure of significant (greater than 10(5) bacteria per ml) gram-negative bacteriuria, and the results of 190 LAL assays w...

journal_title：Journal of clinical microbiology

authors： Nachum R,Shanbrom E

更新日期：1981-01-01 00:00:00

abstract：:To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as indicated by the abse...

journal_title：Journal of clinical microbiology

authors： Schoeb TR,Dybvig K,Keisling KF,Davidson MK,Davis JK

更新日期：1997-07-01 00:00:00

abstract：:Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both...

journal_title：Journal of clinical microbiology

authors： Honda T,Sornchai C,Takeda Y,Miwatani T

更新日期：1982-10-01 00:00:00

abstract：:Thirty-seven bacterial clones producing human recombinant monoclonal antibody Fab fragments (rFabs) reactive to herpes simplex virus (HSV) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an HSV lysate. Thirty-four of the HSV-specif...

journal_title：Journal of clinical microbiology

authors： Cattani P,Rossolini GM,Cresti S,Santangelo R,Burton DR,Williamson RA,Sanna PP,Fadda G

更新日期：1997-06-01 00:00:00

abstract：:Because of their biofilm-forming capacity, invasive Staphylococcus epidermidis isolates, which cause the majority of nosocomial catheter-related bloodstream infections (BSIs), are thought to be selected at the time of catheter insertion from a population of less virulent commensal strains. This fact allows the predict...

journal_title：Journal of clinical microbiology

authors： Rohde H,Kalitzky M,Kröger N,Scherpe S,Horstkotte MA,Knobloch JK,Zander AR,Mack D

更新日期：2004-12-01 00:00:00

abstract：:Total immunoglobulin E (IgE) and specific IgE were assayed by a radioimmunosorbent test and a reverse enzyme immunoassay in patients with hookworm infections before and after treatment. A total of 77 subjects (30 patients with hookworm infections and 47 subjects as controls) were studied. Both specific and total IgE l...

journal_title：Journal of clinical microbiology

authors： Ganguly NK,Mahajan RC,Sehgal R,Shetty P,Dilawari JB

更新日期：1988-04-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:A 42-year-old woman developed severe, recurrent breast abscesses caused by Corynebacterium minutissimum. Prior reports of C. minutissimum infection have been limited to erythrasma, a minor dermatosis. The microbiological and clinical features of this species were reviewed. ...

journal_title：Journal of clinical microbiology

authors： Berger SA,Gorea A,Stadler J,Dan M,Zilberman M

更新日期：1984-12-01 00:00:00

abstract：:Restriction endonucleases are bacterial enzymes that cleave DNA at specific sites. The resulting DNA fragments may be separated electrophoretically in gel to form specific restriction patterns. In the present study, the restriction endonuclease method was successfully adapted to the analysis of the chromosomal DNA of ...

journal_title：Journal of clinical microbiology

authors： Bjorvatn B,Lund V,Kristiansen BE,Korsnes L,Spanne O,Lindqvist B

更新日期：1984-06-01 00:00:00

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation pro...

journal_title：Journal of clinical microbiology

authors： Xu M,Chan Y,Fischer SH,Remaley AT

更新日期：2004-01-01 00:00:00

abstract：:Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...

journal_title：Journal of clinical microbiology

authors： Kurosaki Y,Grolla A,Fukuma A,Feldmann H,Yasuda J

更新日期：2010-07-01 00:00:00

abstract：:When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

journal_title：Journal of clinical microbiology

authors： Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

更新日期：1998-11-01 00:00:00

abstract：:During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1983-01-01 00:00:00

abstract：:To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...

journal_title：Journal of clinical microbiology

authors： Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

更新日期：2004-09-01 00:00:00

abstract：:This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique ...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Hewitt PL,Allega JM,Keen A,Ruoff KL,Ferraro MJ

更新日期：1998-04-01 00:00:00

abstract：:We used genotypic and phenotypic assays to estimate the frequency of X4/DM viruses in 131 patients infected with non-subtype-B viruses at the time of primary HIV-1 infection (PHI). All patients were enrolled in the French PRIMO Cohort from 1996 to 2007. Most strains belonged to CRF02_AG (51.1%) and subtype A (14.5%). ...

journal_title：Journal of clinical microbiology

authors： Frange P,Chaix ML,Raymond S,Galimand J,Deveau C,Meyer L,Goujard C,Rouzioux C,Izopet J,French ANRS CO 06 PRIMO Cohort Study Group.

更新日期：2010-10-01 00:00:00

abstract：:Infected aneurysms caused by Yersinia are very uncommon and are principally due to Yersinia enterocolitica. We describe the first case of an infected aneurysm caused by Yersinia pseudotuberculosis in an elderly patient with a history of atherosclerotic cardiovascular disease. ...

journal_title：Journal of clinical microbiology

authors： Hadou T,Elfarra M,Alauzet C,Guinet F,Lozniewski A,Lion C

更新日期：2006-09-01 00:00:00

abstract：:Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...

journal_title：Journal of clinical microbiology

authors： Ascher DP,Wilson S,Fischer GW

更新日期：1991-12-01 00:00:00