Brevibacterium casei sepsis in an 18-year-old female with AIDS.

abstract：:Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans infection in whom the same strain was maintained, one patient in whom the infecting strain was replaced, and their male s...

journal_title：Journal of clinical microbiology

authors： Schröppel K,Rotman M,Galask R,Mac K,Soll DR

更新日期：1994-11-01 00:00:00

abstract：:We report disseminated adenovirus (ADV) infection in four adult cancer patients presenting with focal pulmonary consolidation. In all cases, ADV was recovered from respiratory specimens and ADV viremia (>1 × 10(5) copies/ml) was determined by a quantitative PCR assay. Despite antiviral therapy, 3 (75%) patients died. ...

journal_title：Journal of clinical microbiology

authors： Lee YJ,Palomino-Guilen P,Babady NE,Lamson DM,St George K,Tang YW,Papanicolaou GA

更新日期：2014-01-01 00:00:00

abstract：:Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

journal_title：Journal of clinical microbiology

authors： Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

更新日期：2011-12-01 00:00:00

abstract：:We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Myco...

journal_title：Journal of clinical microbiology

authors： Bencina D,Bradbury JM

更新日期：1992-02-01 00:00:00

abstract：:At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds had completely healed, without complicated infections, when the cellu...

journal_title：Journal of clinical microbiology

authors： Kitamura T,Kawamura Y,Ohkusu K,Masaki T,Iwashita H,Sawa T,Fujii S,Okamoto T,Akaike T

更新日期：2007-01-01 00:00:00

abstract：:To provide more sensitive and convenient methods for the detection of equine infectious anemia virus (EIAV), we developed an enzyme-linked immunosorbent assay (ELISA) employing the EIAV gag precursor (Pr55gag) produced by using recombinant DNA techniques. The antigenic reactivity of the recombinant EIAV Pr55gag was fo...

journal_title：Journal of clinical microbiology

authors： Archambault D,Wang ZM,Lacal JC,Gazit A,Yaniv A,Dahlberg JE,Tronick SR

更新日期：1989-06-01 00:00:00

abstract：:Rhodotorula infections occur among patients with immunosuppression and/or central venous catheters. Using standardized methods (NCCLS M27-A), we determined the antifungal susceptibilities of 10 Rhodotorula bloodstream infection isolates. Patient information was collected for clinical correlation. The MICs of amphoteri...

journal_title：Journal of clinical microbiology

authors： Zaas AK,Boyce M,Schell W,Lodge BA,Miller JL,Perfect JR

更新日期：2003-11-01 00:00:00

abstract：:The diagnosis of congenital syphilis in apparently healthy infants continues to be problematic. Immunoglobulin M antibodies specific for a subset of Treponema pallidum antigens have been detected by Western blotting (immunoblotting). In the present study we investigated the sensitivity and specificity of this method. ...

journal_title：Journal of clinical microbiology

authors： Meyer MP,Eddy T,Baughn RE

更新日期：1994-03-01 00:00:00

abstract：:This study estimated the regional and age- and gender-specific seroprevalences of herpes simplex virus type 1 (HSV-1) and HSV-2 in Ontario, Canada. Stored serum specimens from subjects aged 15 to 44 years, including men (n = 979), women not under prenatal care (n = 638), and women under prenatal care (n = 701) submitt...

journal_title：Journal of clinical microbiology

authors： Howard M,Sellors JW,Jang D,Robinson NJ,Fearon M,Kaczorowski J,Chernesky M

更新日期：2003-01-01 00:00:00

abstract：:The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal gr...

journal_title：Journal of clinical microbiology

authors： Murray PR,Wold AD,Schreck CA,Washington JA II

更新日期：1976-07-01 00:00:00

abstract：:We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...

journal_title：Journal of clinical microbiology

authors： Peel MM,Hornidge KA,Luppino M,Stacpoole AM,Weaver RE

更新日期：1991-11-01 00:00:00

abstract：:Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...

journal_title：Journal of clinical microbiology

authors： Tanner AC,Mathney JM,Kent RL,Chalmers NI,Hughes CV,Loo CY,Pradhan N,Kanasi E,Hwang J,Dahlan MA,Papadopolou E,Dewhirst FE

更新日期：2011-04-01 00:00:00

abstract：:Since January 1988, Staphylococcus aureus strains with a high level of quinolone resistance have been isolated at 17 hospitals and 15 nursing homes in New York City. The majority of these strains were methicillin resistant. The bacteriophage types and susceptibility to other antibiotics were similar to those of quinol...

journal_title：Journal of clinical microbiology

authors： Schaefler S

更新日期：1989-02-01 00:00:00

abstract：:In the context of hematopoietic stem cell transplantation, adenovirus infections are associated with relevant mortality and morbidity. Detection of adenovirus DNA by quantitative PCR is the "gold standard" for these patients. A total of 150 samples, namely, 78 whole-blood, 22 cerebrospinal fluid, 24 digestive biopsy, ...

journal_title：Journal of clinical microbiology

authors： Jeulin H,Salmon A,Bordigoni P,Venard V

更新日期：2010-09-01 00:00:00

abstract：:We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 mo...

journal_title：Journal of clinical microbiology

authors： Liebana E,Smith RP,Batchelor M,McLaren I,Cassar C,Clifton-Hadley FA,Paiba GA

更新日期：2005-02-01 00:00:00

abstract：:A heminested inverse PCR (HIP) for the amplification of sequences flanking the Mycobacterium tuberculosis insertion sequence IS6110 has been developed. The method depends upon primers that anneal to IS6110 at sites between its 5' end and the closest BsrFI site. The accuracy of HIP was demonstrated by the amplification...

journal_title：Journal of clinical microbiology

authors： Patel S,Wall S,Saunders NA

更新日期：1996-07-01 00:00:00

abstract：:A serotyping scheme for Enterobacter cloacae based on heat-stable somatic antigens is described. A total of 28 antisera were prepared in rabbits, and titers of agglutinins were high (greater than 640). Some cross-reactions were observed, and 11 sera required absorption before routine use. Of 300 clinical isolates from...

journal_title：Journal of clinical microbiology

authors： Gaston MA,Bucher C,Pitt TL

更新日期：1983-11-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19). While molecular-based testing is used to diagnose COVID-19, serologic testing of antibodies specific to SARS-CoV-2 is used to detect past infection. While most serologic assays are qualitativ...

journal_title：Journal of clinical microbiology

authors： Higgins V,Fabros A,Kulasingam V

更新日期：2021-01-22 00:00:00

abstract：:A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude bi...

journal_title：Journal of clinical microbiology

authors： Arita M

更新日期：2013-08-01 00:00:00

abstract：:Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value. ...

journal_title：Journal of clinical microbiology

authors： Jantzen E,Berdal BP,Omland T

更新日期：1979-12-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) has occasionally been associated with cases of interstitial pneumonitis, mainly in individuals with impaired cellular immunity. Here we report for the first time severe interstitial pneumonitis with simultaneous HHV-6 and Pneumocystis carinii infections in the lung tissue of a young patient...

journal_title：Journal of clinical microbiology

authors： Vuorinen T,Kotilainen P,Lautenschlager I,Kujari H,Krogerus L,Oksi J

更新日期：2004-11-01 00:00:00

abstract：:A case of severe progressive subcutaneous abscesses and necrotizing tenosynovitis of the right arm of a 30-year-old woman caused by Rhodococcus aurantiacus is reported. ...

journal_title：Journal of clinical microbiology

authors： Tsukamura M,Hikosaka K,Nishimura K,Hara S

更新日期：1988-02-01 00:00:00

abstract：:Visceral and cutaneous leishmaniases are heterogenous entities. The Leishmania species that a given patient harbors usually cannot be determined clinically, and this identification is essential to prescribe the best species-specific therapeutic regimen. Our diagnosis procedure includes a real-time PCR assay targeted a...

journal_title：Journal of clinical microbiology

authors： Foulet F,Botterel F,Buffet P,Morizot G,Rivollet D,Deniau M,Pratlong F,Costa JM,Bretagne S

更新日期：2007-07-01 00:00:00

abstract：:A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clini...

journal_title：Journal of clinical microbiology

authors： Pouletty P,Chomel JJ,Thouvenot D,Catalan F,Rabillon V,Kadouche J

更新日期：1987-05-01 00:00:00

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:Antigens of Rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. Immunoblots of whole-cell antigen preparations of clinical isolates of R. equi revealed that major protein bands with molecular masses of 15 to 17 kDa were present in all clinical isolates tested and all isolates virulent f...

journal_title：Journal of clinical microbiology

authors： Takai S,Koike K,Ohbushi S,Izumi C,Tsubaki S

更新日期：1991-03-01 00:00:00

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin ...

journal_title：Journal of clinical microbiology

authors： Rodda SJ,Gallichio HA,Hampson AW

更新日期：1981-11-01 00:00:00