Effects of selective media and atmosphere of incubation on the isolation of group A streptococci.

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

journal_title：Journal of clinical microbiology

authors： Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

更新日期：1984-09-01 00:00:00

abstract：:Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

journal_title：Journal of clinical microbiology

authors： Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

更新日期：2003-06-01 00:00:00

abstract：:Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis....

journal_title：Journal of clinical microbiology

authors： Fukunaga M,Sohnaka M,Takahashi Y,Nakao M,Miyamoto K

更新日期：1993-05-01 00:00:00

abstract：:Seventy-nine methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated during 1980 to 1990, were classified as MRSA Aggl- (14 strains) and MRSA Aggl+ (65 strains) strains on the basis of test results in slide agglutination assays designed to detect fibrinogen-binding protein (clumping factor) and protein A ...

journal_title：Journal of clinical microbiology

authors： Kuusela P,Hildén P,Savolainen K,Vuento M,Lyytikäinen O,Vuopio-Varkila J

更新日期：1994-01-01 00:00:00

abstract：:Dogs orally infected with Neorickettsia helminthoeca developed immunoglobulin G titers against Erlichia risticii, Erlichia sennetsu, and Erlichia canis similar to those against N. helminthoeca antigen, as determined by immunofluorescence. Western immunoblotting showed that the major common antigens shared among the mi...

journal_title：Journal of clinical microbiology

authors： Rikihisa Y

更新日期：1991-09-01 00:00:00

abstract：:A new case of Campylobacter hyointestinalis-associated diarrhea in a human is reported. The medical history of the patient was significant for immunodeficiency because of an evolutive chronic myeloid leukemia. The diarrhea rapidly stopped after administration of oral erythromycin. No other enteropathogenic agent was f...

journal_title：Journal of clinical microbiology

authors： Minet J,Grosbois B,Megraud F

更新日期：1988-12-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:Using blaZ PCR as the "gold standard," the sensitivities of CLSI penicillin zone edge and nitrocefin-based tests for β-lactamase production in Staphylococcus aureus were 64.5% and 35.5%, respectively, with specificity of 99.8% for both methods. In 2013, 13.5% of 3,083 S. aureus isolates from 31 U.S. centers were penic...

journal_title：Journal of clinical microbiology

authors： Richter SS,Doern GV,Heilmann KP,Miner S,Tendolkar S,Riahi F,Diekema DJ

更新日期：2016-03-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...

journal_title：Journal of clinical microbiology

authors： Faulde M,Putzker M,Mertes T,Sobe D

更新日期：1991-02-01 00:00:00

abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

journal_title：Journal of clinical microbiology

authors： Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

更新日期：2012-12-01 00:00:00

abstract：:Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Unicomb LE,Bishop RF

更新日期：1987-01-01 00:00:00

abstract：:With use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 Escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either E. coli or Vibrio cholerae. These findings suggest marked ...

journal_title：Journal of clinical microbiology

authors： Sack RB,Froehlich JL

更新日期：1977-06-01 00:00:00

abstract：:Three variants of a Corynebacterium diphtheriae subsp. mitis strain belfanti, two toxigenic (phage types 7 and 11) and one nontoxigenic (phage type 7), were simultaneously isolated from the throat of an adult. ...

journal_title：Journal of clinical microbiology

authors： Chang DN,Laughren GS,Chalvardjian NE

更新日期：1978-12-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:Seven rotavirus strains dually reactive to serotype G1- and G2-specific monoclonal antibodies (MAbs) from hospitalized children with rotavirus diarrhea were culture adapted. Six strains were neutralized with G1 antiserum to a higher titer than that of G2, and one was neutralized with G1 and G2 antisera to equal titers...

journal_title：Journal of clinical microbiology

authors： Ranshing SS,Kelkar SD

更新日期：2003-11-01 00:00:00

abstract：:Aspergillus fumigatus has widely evolved resistance to the most commonly used class of antifungal chemicals, the azoles. Current methods for identifying azole resistance are time-consuming and depend on specialized laboratories. There is an urgent need for rapid detection of these emerging pathogens at point-of-care t...

journal_title：Journal of clinical microbiology

authors： Yu LS,Rodriguez-Manzano J,Moser N,Moniri A,Malpartida-Cardenas K,Miscourides N,Sewell T,Kochina T,Brackin A,Rhodes J,Holmes AH,Fisher MC,Georgiou P

更新日期：2020-10-21 00:00:00

abstract：:Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

journal_title：Journal of clinical microbiology

authors： Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

更新日期：1996-08-01 00:00:00

abstract：:Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...

journal_title：Journal of clinical microbiology

authors： Denyer SP,Davies MC,Evans JA,Finch RG,Smith DG,Wilcox MH,Williams P

更新日期：1990-08-01 00:00:00

abstract：:A member of the Enterobacteriaceae initially identified as Kluyvera cryocrescens by the MicroScan Gram-Negative Combo 13 panel caused an outbreak of nosocomial infections in four patients (pneumonia, n = 2; urinary tract infection, n = 1; wound infection, n = 1) and urinary tract colonization in one patient. When the ...

journal_title：Journal of clinical microbiology

authors： Warren JR,Farmer JJ 3rd,Dewhirst FE,Birkhead K,Zembower T,Peterson LR,Sims L,Bhattacharya M

更新日期：2000-11-01 00:00:00

abstract：:A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

journal_title：Journal of clinical microbiology

authors： Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

更新日期：2017-12-01 00:00:00

abstract：:Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

journal_title：Journal of clinical microbiology

authors： Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

更新日期：2016-12-01 00:00:00

abstract：:Rotavirus RNA was detected in the cerebrospinal fluid (CSF) of a child with central nervous system disease symptoms associated with rotavirus gastroenteritis. The rotavirus isolates from the fecal and CSF samples were genotyped as G1P[8]. Sequence analysis of the VP7 and VP4 proteins derived from the fecal and CSF sam...

journal_title：Journal of clinical microbiology

authors： Iturriza-Gómara M,Auchterlonie IA,Zaw W,Molyneaux P,Desselberger U,Gray J

更新日期：2002-12-01 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00

abstract：:During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibo...

journal_title：Journal of clinical microbiology

authors： Bakerman P,Balasuriya L,Fried O,Tellez D,Garcia-Filion P,Dalton H

更新日期：2011-10-01 00:00:00

abstract：:The elimination of leprosy continues to be a challenge, with the disease remaining endemic in several countries. India accounts for the highest number of cases, and the identification of child cases indicates recent transmission. Genetic markers, like variable-number tandem repeats (VNTRs) and single-nucleotide polymo...

journal_title：Journal of clinical microbiology

authors： Kuruwa S,Vissa V,Mistry N

更新日期：2012-04-01 00:00:00

abstract：:Several media were used to evaluate the hemolytic activity of Listeria monocytogenes, and this property was used along with the API Listeria system to identify Listeria spp. All L. monocytogenes strains were identified correctly with this system, and blood agar base no. 2 and Columbia blood agar base supplemented with...

journal_title：Journal of clinical microbiology

authors： Fujisawa T,Mori M

更新日期：1994-04-01 00:00:00