Antigenic similarity of heat-labile enterotoxins from diverse strains of Escherichia coli.

abstract：:We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

journal_title：Journal of clinical microbiology

authors： Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

更新日期：1996-12-01 00:00:00

abstract：:Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis. A total of three patients of Plasmodium ovale infection were detected, but PCR-amplified DNA of the P. ovale isolate...

journal_title：Journal of clinical microbiology

authors： Kawamoto F,Miyake H,Kaneko O,Kimura M,Nguyen TD,Nguyen TD,Liu Q,Zhou M,Le DD,Kawai S,Isomura S,Wataya Y

更新日期：1996-09-01 00:00:00

abstract：:Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacryla...

journal_title：Journal of clinical microbiology

authors： Saengjaruk P,Chaicumpa W,Watt G,Bunyaraksyotin G,Wuthiekanun V,Tapchaisri P,Sittinont C,Panaphut T,Tomanakan K,Sakolvaree Y,Chongsa-Nguan M,Mahakunkijcharoen Y,Kalambaheti T,Naigowit P,Wambangco MA,Kurazono H,Hayashi H

更新日期：2002-02-01 00:00:00

abstract：:Eight strains of a new intermediate adenovirus were isolated in the course of an investigation of conjunctivitis in Hiroshima City, Japan. The strain was first isolated in July 1986. All eight strains were isolated from conjunctival swab samples from patients with conjunctivitis, mainly epidemic keratoconjunctivitis. ...

journal_title：Journal of clinical microbiology

authors： Noda M,Miyamoto Y,Ikeda Y,Matsuishi T,Ogino T

更新日期：1991-07-01 00:00:00

abstract：:Rhodococcus equi infection is increasing in regions with high HIV prevalence worldwide. The microbiological features and clinical mimicry of tuberculosis infection pose diagnostic challenges in high-tuberculosis-incidence settings. We present two HIV-associated cases of R. equi infection from Vietnam and discuss the u...

journal_title：Journal of clinical microbiology

authors： Le T,Cash-Goldwasser S,Tho PV,Lan NP,Campbell JI,van Doorn HR,Lam NT,Trung NV,Trinh DT,Van Kinh N,Wertheim HF

更新日期：2015-04-01 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:A 44-year-old diabetic female presented to a hospital in Jamaica with thermal burns. Trichosporon asahii was isolated from facial wounds, sputum, and a meningeal swab. Dissemination of the fungus was demonstrated in stained histological sections of the meninges and a brain abscess at autopsy. Pure growth of the fungus...

journal_title：Journal of clinical microbiology

authors： Heslop OD,Nyi Nyi MP,Abbott SP,Rainford LE,Castle DM,Coard KC

更新日期：2011-12-01 00:00:00

abstract：:We conducted a Linear Array test/retest analysis using cytologic specimens from 198 women. A total of 67.2% of samples had the same human papillomavirus (HPV) types detected in both tests (type-specific positive agreement was 83.3% overall [Kappa = 0.9] and 86.8% for carcinogenic types [Kappa = 0.92]). Discordance was...

journal_title：Journal of clinical microbiology

authors： Koshiol J,Dunn ST,Walker JL,Zuna RE,Schiffman M,Sherman ME,Gold MA,Allen RA,Zhang R,Wang SS,Wentzensen N

更新日期：2013-02-01 00:00:00

abstract：:We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-bui...

journal_title：Journal of clinical microbiology

authors： Sleiman S,Halliday CL,Chapman B,Brown M,Nitschke J,Lau AF,Chen SC

更新日期：2016-08-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:An in vivo model of hub-related catheter sepsis in rabbits is reported. The model was used to investigate the protection offered by a new hub design against external contamination by Pseudomonas aeruginosa or Staphylococcus epidermidis and to reassess the diagnostic value of the semiquantitative culture method in bact...

journal_title：Journal of clinical microbiology

authors： Segura M,Alía C,Valverde J,Franch G,Torres Rodríguez JM,Sitges-Serra A

更新日期：1990-11-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00

abstract：:Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comp...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodriguez-Granger J,Cueto-Lopez M,Sampedro A,Gaye EB,Haro JM,Andreu A

更新日期：1999-08-01 00:00:00

abstract：:The detection and quantitation of human parainfluenza virus type 1 (HPIV-1) RNA in nasal wash specimens from 49 children with lower respiratory infections were performed by a reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA). The HPIV-1 RT-PCR-EHA was then used to test 40 samples from asymptomatic chil...

journal_title：Journal of clinical microbiology

authors： Fan J,Henrickson KJ

更新日期：1996-08-01 00:00:00

abstract：:The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

journal_title：Journal of clinical microbiology

authors： Garrett MA,Holmes HT,Nolte FS

更新日期：1992-07-01 00:00:00

abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Sampson-Scherer J

更新日期：1977-03-01 00:00:00

abstract：:Cross-reactivity between Legionella spp. and Capnocytophaga ochracea was noted by latex agglutination tests (Serobact Legionella; Disposable Products, Adelaide, Australia). Four of 11 (36%) C. ochracea isolates agglutinated with latex reagents designed to identify Legionella pneumophila serogroups. C. ochracea isolate...

journal_title：Journal of clinical microbiology

authors： Chen S,Hicks L,Yuen M,Mitchell D,Gilbert GL

更新日期：1994-12-01 00:00:00

abstract：:Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing...

journal_title：Journal of clinical microbiology

authors： Pluquet E,Arlet G,Bingen E,Drieux L,Mammeri H

更新日期：2011-07-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:False sensitivities were occasionally encountered in antibiotic-free chambers of the Autobac I system. This unusual phenomenon occurred randomly among gram-negative bacilli and appears to be colistin-related. ...

journal_title：Journal of clinical microbiology

authors： Duckenfield L,Wadke M,Ashton JK

更新日期：1980-10-01 00:00:00

abstract：:Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In...

journal_title：Journal of clinical microbiology

authors： Low HC,Silver MI,Brown BJ,Leng CY,Blas MM,Gravitt PE,Woo YL

更新日期：2015-02-01 00:00:00

abstract：:White-footed mice, Peromyscus leucopus, were captured in southern Connecticut during 1997 and 1998 to determine the prevalence of infections caused by granulocytic Ehrlichia sp., Borrelia burgdorferi, and Babesia microti. Of the 50 mice captured and recaptured, 25 of 47 (53.2%) and 23 of 48 (47.9%) contained antibodie...

journal_title：Journal of clinical microbiology

authors： Stafford KC 3rd,Massung RF,Magnarelli LA,Ijdo JW,Anderson JF

更新日期：1999-09-01 00:00:00

abstract：:In the last decade, the global emergence of carbapenem resistance in Enterobacteriaceae has posed great concern to public health. Data concerning the role of environmental contamination in the dissemination of carbapenem-resistant Enterobacteriaceae (CRE) are currently lacking. Here, we aimed to examine the extent of ...

journal_title：Journal of clinical microbiology

authors： Lerner A,Adler A,Abu-Hanna J,Meitus I,Navon-Venezia S,Carmeli Y

更新日期：2013-01-01 00:00:00

abstract：:The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

journal_title：Journal of clinical microbiology

authors： Helweg-Larsen J,Lundgren B,Lundgren JD

更新日期：2001-10-01 00:00:00

abstract：:An 840-bp fragment of the 18S rRNA gene was used to identify Cryptosporidium spp. recovered from human immunodeficiency virus (HIV)-infected and -uninfected patients from Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. Initial identification was by Ziehl-Neelsen acid-fast staining. Confirmation was by nested P...

journal_title：Journal of clinical microbiology

authors： Gatei W,Greensill J,Ashford RW,Cuevas LE,Parry CM,Cunliffe NA,Beeching NJ,Hart CA

更新日期：2003-04-01 00:00:00

abstract：:A total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in Matlab, Bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (EIA). EIA-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (Ad40) and...

journal_title：Journal of clinical microbiology

authors： Jarecki-Khan K,Tzipori SR,Unicomb LE

更新日期：1993-03-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:Bacteriological and morphological studies of 38 brown pigment common duct stones were performed. Stone cultures were positive for bacteria in 80.5% of those studied. Enterococci were the most common organisms that were isolated. Scanning electron microscopy showed the presence of bacteria in 84.2% of the stones. The b...

journal_title：Journal of clinical microbiology

authors： Leung JW,Sung JY,Costerton JW

更新日期：1989-05-01 00:00:00

abstract：:The present study describes the use of the automated BACTEC 9240 blood culture system, the Serum Separator Tube (SST), and the BD PHOENIX Automated Microbiology System in combination for the direct identification and antimicrobial susceptibility testing (AST) of gram-negative rods (GNRs) from positive blood cultures (...

journal_title：Journal of clinical microbiology

authors： Funke G,Funke-Kissling P

更新日期：2004-04-01 00:00:00

abstract：:Asymptomatic Epstein-Barr virus (EBV) reactivations periodically occur in oral mucosa-associated lymphoid tissues. Until now, EBV reactivation has been diagnosed by serologic profiles that suggest virus replication. Serologic responses, however, are delayed and do not necessarily indicate ongoing replicative activity....

journal_title：Journal of clinical microbiology

authors： Maurmann S,Fricke L,Wagner HJ,Schlenke P,Hennig H,Steinhoff J,Jabs WJ

更新日期：2003-12-01 00:00:00