Regional distribution of antibodies to herpes simplex virus type 1 (HSV-1) and HSV-2 in men and women in Ontario, Canada.

abstract：:The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar ech...

journal_title：Journal of clinical microbiology

authors： Liance M,Janin V,Bresson-Hadni S,Vuitton DA,Houin R,Piarroux R

更新日期：2000-10-01 00:00:00

abstract：:A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

journal_title：Journal of clinical microbiology

authors： Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2008-08-01 00:00:00

abstract：:We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and spec...

journal_title：Journal of clinical microbiology

authors： Zepeda-Lopez HM,Gonzalez-Lugo GM

更新日期：1995-05-01 00:00:00

abstract：:A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

journal_title：Journal of clinical microbiology

authors： Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

更新日期：2006-01-01 00:00:00

abstract：:A 24-year-old man sustained a crush injury to the tibia, which subsequently became infected with Saksenaea vasiformis. He was treated with debridement and a free myocutaneous flap, but amputation was necessary because of mycotic osteomyelitis. S. vasiformis was recovered and identified on the basis of its characterist...

journal_title：Journal of clinical microbiology

authors： Pierce PF,Wood MB,Roberts GD,Fitzgerald RH Jr,Robertson C,Edson RS

更新日期：1987-05-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

journal_title：Journal of clinical microbiology

authors： Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

更新日期：2007-02-01 00:00:00

abstract：:Hepatitis E virus (HEV) genotype 3 is endemic in Europe and hyperendemic in southern France. Recent reports of a high prevalence of HEV RNA in blood donations and in culinary specialties from this geographical area confirmed the endemicity of HEV and sources of viral transmission in this geographical area. HEV causes ...

journal_title：Journal of clinical microbiology

authors： Moal V,Legris T,Motte A,Vacher-Coponat H,Fages L,Jourde-Chiche N,Borentain P,Jaubert D,Gerolami R,Colson P

更新日期：2015-05-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:A fully automated microparticle enzyme immunoassay (EIA), IMx Core, was developed for the detection of antibody against hepatitis B core antigen (anti-HBc). IMx Core sensitivity was less than 0.5 Paul Ehrlich Institut units per ml and was greater than that of the commercial radioimmunoassay (RIA) or EIA, Corab and Cor...

journal_title：Journal of clinical microbiology

authors： Spronk AM,Schmidt L,Krenc C,Pavlis-Jenkins L,Brady J,Taskar S,Angus-Finn L,Mimms L

更新日期：1991-03-01 00:00:00

abstract：:In this report, we present a case of rhino-orbital mucormycosis in a 57-year-old female with poorly controlled diabetes mellitus. The only mold cultured at 25°C, 37°C, and 40°C from a specimen of the nasal crust was identified phenotypically and independently using nuclear ribosomal DNA sequence data as Thamnostylum l...

journal_title：Journal of clinical microbiology

authors： Xess I,Mohapatra S,Shivaprakash MR,Chakrabarti A,Benny GL,O'Donnell K,Padhye AA

更新日期：2012-04-01 00:00:00

abstract：:A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus (VZV) in 140 dermal lesions from 133 patients. A total of 79 (56%) specimens were positive for VZV: 40 (51%) by DFA alone, 2 (3%...

journal_title：Journal of clinical microbiology

authors： Coffin SE,Hodinka RL

更新日期：1995-10-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...

journal_title：Journal of clinical microbiology

authors： Wellinghausen N,Kochem AJ,Disqué C,Mühl H,Gebert S,Winter J,Matten J,Sakka SG

更新日期：2009-09-01 00:00:00

abstract：:Although numerous perioperative samples and culture media are required to diagnose prosthetic joint infection (PJI), their exact number and types have not yet been definitely determined with a high level of proof. We conducted a prospective multicenter study to determine the minimal number of samples and culture media...

journal_title：Journal of clinical microbiology

authors： Bémer P,Léger J,Tandé D,Plouzeau C,Valentin AS,Jolivet-Gougeon A,Lemarié C,Kempf M,Héry-Arnaud G,Bret L,Juvin ME,Giraudeau B,Corvec S,Burucoa C,Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Stu

更新日期：2016-02-01 00:00:00

abstract：:A total of 101 food-borne and waterborne outbreaks that were caused by norovirus and that resulted in more than 4,100 cases of illness were reported to the Swedish Institute for Infectious Disease Control from January 2002 to December 2006. Sequence and epidemiological data for isolates from 73 outbreaks were analyzed...

journal_title：Journal of clinical microbiology

authors： Lysén M,Thorhagen M,Brytting M,Hjertqvist M,Andersson Y,Hedlund KO

更新日期：2009-08-01 00:00:00

abstract：:An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the result...

journal_title：Journal of clinical microbiology

authors： Arcavi M,Orfus G,Griemberg G

更新日期：1997-06-01 00:00:00

abstract：:Fast and accurate identification and typing of pathogens are essential for effective surveillance and outbreak detection. The current routine procedure is based on a variety of techniques, making the procedure laborious, time-consuming, and expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge po...

journal_title：Journal of clinical microbiology

authors： Joensen KG,Scheutz F,Lund O,Hasman H,Kaas RS,Nielsen EM,Aarestrup FM

更新日期：2014-05-01 00:00:00

abstract：:Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

journal_title：Journal of clinical microbiology

authors： Vastag M,Papp T,Kasza Z,Vágvölgyi C

更新日期：1998-07-01 00:00:00

abstract：:In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

journal_title：Journal of clinical microbiology

authors： Griffiths TA,Rioux K,De Buck J

更新日期：2008-04-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms...

journal_title：Journal of clinical microbiology

authors： Van TT,Mongkolrattanothai K,Arevalo M,Lustestica M,Dien Bard J

更新日期：2017-05-01 00:00:00

abstract：:Helicobacter pylori was grown in low numbers from the saliva of one of nine patients who were positive for gastric H. pylori. The saliva-derived isolate from this patient was identical to the antral biopsy-derived isolate from the same patient and differed from isolates cultured from the antral biopsies of all other p...

journal_title：Journal of clinical microbiology

authors： Ferguson DA Jr,Li C,Patel NR,Mayberry WR,Chi DS,Thomas E

更新日期：1993-10-01 00:00:00

abstract：:The impact of transport time and temperature on survival of group B streptococci (GBS) in Amies transport medium was evaluated. Viability of 10 or more CFU of GBS was maintained for 4 days at 24 or 3 degrees C. However, there was a significant decrease in viability for GBS held at 30 degrees C for 4 days. ...

journal_title：Journal of clinical microbiology

authors： Stoner KA,Rabe LK,Hillier SL

更新日期：2004-11-01 00:00:00

abstract：:The pathogenic Neisseria species N. meningitidis and N. gonorrhoeae possess an outer membrane lipoprotein, designated Lip, which is present in all strains tested. The predicted protein sequence of Lip consists of a consensus AAEAP amino acid repeat. The objective of this study was to determine the feasibility of using...

journal_title：Journal of clinical microbiology

authors： Trees DL,Schultz AJ,Knapp JS

更新日期：2000-08-01 00:00:00

abstract：:We used several molecular typing methods to analyze 196 methicillin-resistant Staphylococcus aureus (MRSA) and 139 methicillin-susceptible S. aureus (MSSA) isolates collected between 1996 and 2005. The sequence type 72 MRSA has increased in frequency in the community in the Republic of Korea and in hospitals in recent...

journal_title：Journal of clinical microbiology

authors： Kim ES,Lee HJ,Chung GT,Lee YS,Shin DH,Jung SI,Song KH,Park WB,Kim NJ,Park KU,Kim EC,Oh MD,Kim HB

更新日期：2011-05-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:A Campylobacter fetus subsp. venerealis-specific 5' Taq nuclease PCR assay using a 3' minor groove binder-DNA probe (TaqMan MGB) was developed based on a subspecies-specific fragment of unknown identity (S. Hum, K. Quinn, J. Brunner, and S. L. On, Aust. Vet. J. 75:827-831, 1997). The assay specifically detected four C...

journal_title：Journal of clinical microbiology

authors： McMillen L,Fordyce G,Doogan VJ,Lew AE

更新日期：2006-03-01 00:00:00

abstract：:DNA restriction analysis was carried out on a sample of 212 epidemiologically unrelated adenovirus (Ad) strains of serotype 7 isolated in Chile, Uruguay, and Argentina from nasopharyngeal aspirates of children with acute lower respiratory disease between 1984 and 1990. The identified genome types were Ad7b (n = 12), A...

journal_title：Journal of clinical microbiology

authors： Kajon A,Wadell G

更新日期：1994-09-01 00:00:00

abstract：:An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots...

journal_title：Journal of clinical microbiology

authors： Gaafar HA,D'Arcangelis DC

更新日期：1976-04-01 00:00:00

abstract：:While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

journal_title：Journal of clinical microbiology

authors： Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

更新日期：2011-11-01 00:00:00