Effect of transport time, temperature, and concentration on the survival of group B streptococci in amies transport medium.

abstract：:Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory powe...

journal_title：Journal of clinical microbiology

authors： Shi ZY,Liu PY,Lau YJ,Lin YH,HU BS

更新日期：1997-01-01 00:00:00

abstract：:Streptococcus pneumoniae plays an important role in causing acute exacerbations in patients with chronic respiratory disease. However, few data are available regarding pneumococcal persistence in adult patients with chronic respiratory diseases. Fifty pneumococci recovered from sputum samples (1995 to 2010) from 13 ad...

journal_title：Journal of clinical microbiology

authors： Domenech A,Ardanuy C,Balsalobre L,Marti S,Calatayud L,De la Campa AG,Brueggemann AB,Liñares J

更新日期：2012-12-01 00:00:00

abstract：:The zygomycetous mold Cokeromyces recurvatus was isolated from the pleural and peritoneal fluids of a 64-year-old man with a history of peptic ulcer disease and alcohol abuse. The patient presented to the hospital with severe abdominal pain and a ruptured duodenal ulcer. This is one of the few times that C.recurvatus ...

journal_title：Journal of clinical microbiology

authors： Munipalli B,Rinaldi MG,Greenberg SB

更新日期：1996-10-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

journal_title：Journal of clinical microbiology

authors： Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

更新日期：2014-05-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:An enzyme immunoassay (enzyme-linked immunosorbent assay [ELISA]) to detect hamster antibody to toxins A and B of Clostridium difficile was developed in which toxin preparations are used to coat the solid phase. The specificity of the assay was supported by blocking tests with the toxin preparations and proteins from ...

journal_title：Journal of clinical microbiology

authors： Viscidi RP,Yolken RH,Laughon BE,Bartlett JG

更新日期：1983-08-01 00:00:00

abstract：:A new liquid medium (modified Lombard-Dowell broth) was inoculated with stock culture strains of aerobic and anaerobic bacteria and compared with prereduced chopped-meat glucose inoculated with the same anaerobes. Both broths were subcultured at 48 and 72 h to aerobic and anaerobic blood agar plates, and the numbers o...

journal_title：Journal of clinical microbiology

authors： Jessee MT,Robinson PJ

更新日期：1977-11-01 00:00:00

abstract：:The ability of vancomycin resistance determinants to be horizontally transferred within enterococci species is a concern. Identification and characterization of vancomycin-resistant enterococci (VRE) in a clinical isolate have a significant impact on infection control practices. In this study, we describe a clinical i...

journal_title：Journal of clinical microbiology

authors： Eshaghi A,Shahinas D,Li A,Kariyawasam R,Banh P,Desjardins M,Melano RG,Patel SN

更新日期：2015-07-01 00:00:00

abstract：:A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological detail. It may also be used to stain the organism in body fluids. The stain is not specific and thus must be confirmed by d...

journal_title：Journal of clinical microbiology

authors： Faine S,Edelstein P,Kirby BD,Finegold SM

更新日期：1979-07-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:Restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic Escherichia coli. To compare their relative efficiencies, these two sets of probes were radiolabel...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Kalland KH,Raj P,Moseley SL,Bhan MK,Bjorvatn B

更新日期：1988-11-01 00:00:00

abstract：:The sensitive peroxidase-antiperoxidase (PXAPX) method individually and in conjunction with the Papanicolaou (PAP) stain was used to detect herpes simplex virus (HSV) in specimens from human female genitalia. Initial studies using a model system of HSV-1 or HSV-2-infected Vero cells established (i) acetone as the most...

journal_title：Journal of clinical microbiology

authors： Pearson NS,Fleagle G,Docherty JJ

更新日期：1979-11-01 00:00:00

abstract：:A noncompetitive enzyme-linked immunosorbent assay that enables the quantitation of toxic shock syndrome toxin 1 (TSST-1) to as little as 30 pg/ml and the detection of TSST-1 to 10 pg/ml in phosphate-buffered saline including 33% human serum or plasma was developed. It takes only 3 h to complete this assay after plate...

journal_title：Journal of clinical microbiology

authors： Miwa K,Fukuyama M,Kunitomo T,Igarashi H

更新日期：1994-02-01 00:00:00

abstract：:To assess whether rinsing with oral antiseptics before sputum collection would reduce contamination of mycobacterial cultures, 120 patients with suspected tuberculosis were randomly assigned to rinse with chlorhexidine or cetylpyridinium mouthwash before collection. The culture contamination rate was significantly low...

journal_title：Journal of clinical microbiology

authors： Peres RL,Palaci M,Loureiro RB,Dietze R,Johnson JL,Golub JE,Ruffino-Netto A,Maciel EL

更新日期：2011-08-01 00:00:00

abstract：:We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant resul...

journal_title：Journal of clinical microbiology

authors： Saffert RT,Cunningham SA,Ihde SM,Jobe KE,Mandrekar J,Patel R

更新日期：2011-03-01 00:00:00

abstract：:The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI ...

journal_title：Journal of clinical microbiology

authors： Melter O,Aires de Sousa M,Urbásková P,Jakubů V,Zemlicková H,de Lencastre H

更新日期：2003-11-01 00:00:00

abstract：:Reactivation of Epstein-Barr virus (EBV) in early human immunodeficiency virus (HIV) infection was investigated in 49 homosexual men who seroconverted to HIV (cases) as compared with 49 matched controls who remained seronegative to HIV during a longitudinal study. EBV infection was reactivated in cases 6 months, but n...

journal_title：Journal of clinical microbiology

authors： Rahman MA,Kingsley LA,Atchison RW,Belle S,Breinig MC,Ho M,Rinaldo CR Jr

更新日期：1991-06-01 00:00:00

abstract：:Human (n = 33) and canine (n = 53) Campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. We observed 100% typeability and high overall diversity. The majority of human strains (23/33) clustered separately from canine strains, indicating that dogs...

journal_title：Journal of clinical microbiology

authors： Damborg P,Guardabassi L,Pedersen K,Kokotovic B

更新日期：2008-04-01 00:00:00

abstract：:The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; m...

journal_title：Journal of clinical microbiology

authors： Millet J,Miyagi-Shiohira C,Yamane N,Sola C,Rastogi N

更新日期：2007-11-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agg...

journal_title：Journal of clinical microbiology

authors： Smits HL,van der Hoorn MA,Goris MG,Gussenhoven GC,Yersin C,Sasaki DM,Terpstra WJ,Hartskeerl RA

更新日期：2000-03-01 00:00:00

abstract：:A simple and reliable technique was developed for differentiating Helicobacter pylori strains by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified DNAs. Oligonucleotide primer pairs developed to the urease, 48-kDa stress protein (htrA), and 26-kDa antigen-encoding genes wer...

journal_title：Journal of clinical microbiology

authors： Clayton CL,Kleanthous H,Morgan DD,Puckey L,Tabaqchali S

更新日期：1993-06-01 00:00:00

abstract：:Pyrazinamide is important in tuberculosis treatment, as it is bactericidal to semidormant mycobacteria not killed by other antituberculosis drugs. Pyrazinamide is also one of the cornerstone drugs retained in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, due to technical difficulties, routine dr...

journal_title：Journal of clinical microbiology

authors： Mphahlele M,Syre H,Valvatne H,Stavrum R,Mannsåker T,Muthivhi T,Weyer K,Fourie PB,Grewal HM

更新日期：2008-10-01 00:00:00

abstract：:Mycobacterium tuberculosis complex, Mycobacterium avium, and many other nontuberculous mycobacteria are worldwide distributed microorganisms of major medical and veterinary importance. Considering the growing epidemiologic significance of wildlife-livestock-human interrelation, developing rapid detection tools of high...

journal_title：Journal of clinical microbiology

authors： Sevilla IA,Molina E,Elguezabal N,Pérez V,Garrido JM,Juste RA

更新日期：2015-03-01 00:00:00

abstract：:Actinomyces naeslundii is a saprophyte, sometimes a pathogen, of the human oral cavity. Very few extra-oral infections related to this agent have been described. We report the first instance of A. naeslundii as an etiological agent of pelvic actinomycosis in a user of an intrauterine device, an infection so far exclus...

journal_title：Journal of clinical microbiology

authors： Bonnez W,Lattimer G,Mohanraj NA,Johnson TH

更新日期：1985-02-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:We report the first case of maxillary sinusitis caused by Actinomucor elegans in an 11-year-old patient. Histopathological and mycological examinations of surgical maxillary sinuses samples showed coenocytic hyphae characteristic of mucoraceous fungi. The fungi recovered had stolons and rhizoids, nonapophyseal and glo...

journal_title：Journal of clinical microbiology

authors： Davel G,Featherston P,Fernández A,Abrantes R,Canteros C,Rodero L,Sztern C,Perrotta D

更新日期：2001-02-01 00:00:00

abstract：:The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not...

journal_title：Journal of clinical microbiology

authors： Boom WH,Piper DJ,Ruoff KL,Ferraro MJ

更新日期：1985-11-01 00:00:00

abstract：:Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be th...

journal_title：Journal of clinical microbiology

authors： Wahab T,Edvinsson B,Palm D,Lindh J

更新日期：2010-02-01 00:00:00