Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains.

abstract：:The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these diseases within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance of sev...

journal_title：Journal of clinical microbiology

authors： Lamagni TL,Darenberg J,Luca-Harari B,Siljander T,Efstratiou A,Henriques-Normark B,Vuopio-Varkila J,Bouvet A,Creti R,Ekelund K,Koliou M,Reinert RR,Stathi A,Strakova L,Ungureanu V,Schalén C,Strep-EURO Study Group.,Jasir A

更新日期：2008-07-01 00:00:00

abstract：:Serotyping of Chlamydia trachomatis strains usually requires three to six serial passages in shell vials to attain sufficient antigen for typing procedures. To circumvent this problem, we developed a rapid low-passage method for serotyping of C. trachomatis clinical isolates. Isolates with an inclusion count of greate...

journal_title：Journal of clinical microbiology

authors： Suchland RJ,Stamm WE

更新日期：1991-07-01 00:00:00

abstract：:Classifications of the Campylobacter fetus subspecies fetus and venerealis were first described in 1959 and were based on the source of isolation (intestinal versus genital) and the ability of the strains to proliferate in the genital tract of cows. Two phenotypic assays (1% glycine tolerance and H2S production) were ...

journal_title：Journal of clinical microbiology

authors： van der Graaf-van Bloois L,Miller WG,Yee E,Rijnsburger M,Wagenaar JA,Duim B

更新日期：2014-12-01 00:00:00

abstract：:Chronic respiratory tract infections caused by Staphylococcus aureus are common in patients with cystic fibrosis (CF). Recently, it was shown in a few CF patients that S. aureus isolates produce capsular polysaccharides (CPs). However, it is not known whether this is a common feature and whether an immune response to ...

journal_title：Journal of clinical microbiology

authors： Albus A,Fournier JM,Wolz C,Boutonnier A,Ranke M,Høiby N,Hochkeppel H,Döring G

更新日期：1988-12-01 00:00:00

abstract：:Human adenovirus type 4 is one of the major serotypes isolated from patients with adenoviral conjunctivitis. In 2001 we encountered nosocomial infections with epidemic conjunctivitis in the ophthalmology ward of one hospital in Sapporo, which is in the northern part of Japan. Adenoviruses were isolated from the patien...

journal_title：Journal of clinical microbiology

authors： Ariga T,Shimada Y,Ohgami K,Tagawa Y,Ishiko H,Aoki K,Ohno S

更新日期：2004-08-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00

abstract：:A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infe...

journal_title：Journal of clinical microbiology

authors： Olson K,Blair C,Padmanabhan R,Beaty B

更新日期：1988-03-01 00:00:00

abstract：:This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

更新日期：2002-10-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls w...

journal_title：Journal of clinical microbiology

authors： Apfalter P,Blasi F,Boman J,Gaydos CA,Kundi M,Maass M,Makristathis A,Meijer A,Nadrchal R,Persson K,Rotter ML,Tong CY,Stanek G,Hirschl AM

更新日期：2001-02-01 00:00:00

abstract：:Thirteen different serogroup strains of Yersinia pseudotuberculosis and two strains of Yersinia enterocolitica O:3 were examined for the presence of plasmids and plasmid-mediated properties, calcium growth dependency, and autoagglutination. Two Y. enterocolitica strains and eight serogroup (IA, IIA, IIC, III, IVA, VB,...

journal_title：Journal of clinical microbiology

authors： Ishiguro N,Nakaoka Y,Sato G,Tsubokura M

更新日期：1985-04-01 00:00:00

abstract：:COVID-19-associated pulmonary aspergillosis (CAPA) was recently reported as a potential infective complication affecting critically ill patients with acute respiratory distress syndrome following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, with incidence rates varying from 8 to 33% dependin...

journal_title：Journal of clinical microbiology

authors： Borman AM,Palmer MD,Fraser M,Patterson Z,Mann C,Oliver D,Linton CJ,Gough M,Brown P,Dzietczyk A,Hedley M,McLachlan S,King J,Johnson EM

更新日期：2020-12-17 00:00:00

abstract：:By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B su...

journal_title：Journal of clinical microbiology

authors： Zhang W,Bielaszewska M,Kuczius T,Karch H

更新日期：2002-04-01 00:00:00

abstract：:Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern an...

journal_title：Journal of clinical microbiology

authors： Migheli Q,Balmas V,Harak H,Sanna S,Scherm B,Aoki T,O'Donnell K

更新日期：2010-04-01 00:00:00

abstract：:We conducted a case-control study using the Fungitell assay, the novel Platelia Candida Antigen (Ag) Plus and Candida Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1→3)-β-D-glucan (BDG), mannan antigen with/without anti-mannan antibody, and Cand-Tec Candida antigen...

journal_title：Journal of clinical microbiology

authors： Held J,Kohlberger I,Rappold E,Busse Grawitz A,Häcker G

更新日期：2013-04-01 00:00:00

abstract：:A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope ...

journal_title：Journal of clinical microbiology

authors： Islam D,Lindberg AA

更新日期：1992-11-01 00:00:00

abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...

journal_title：Journal of clinical microbiology

authors： Barnishan J,Ayers LW

更新日期：1979-02-01 00:00:00

abstract：:Three antigenic variants of the H3N2 subtype of wild-type influenza A virus (representing the years 1968, 1972, and 1974) were examined for their sensitivity to interferon and for their ability to induce local respiratory tract interferon in volunteers. In addition, the time of appearance of symptoms in infected volun...

journal_title：Journal of clinical microbiology

authors： Richman DD,Murphy BR,Baron S,Uhlendorf C

更新日期：1976-03-01 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00

abstract：:Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

journal_title：Journal of clinical microbiology

authors： Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

更新日期：1996-05-01 00:00:00

abstract：:The performance of three line blot assays (LBAs), the Linear Array HPV genotyping assay (LA) (Roche Diagnostics), INNO-LiPA HPV Genotyping Extra (LiPA) (Innogenetics), and the reverse hybridization assay (RH) (Qiagen), was evaluated using quantitated whole genomic human papillomavirus (HPV) plasmids (types 6, 11, 16, ...

journal_title：Journal of clinical microbiology

authors： Steinau M,Onyekwuluje JM,Scarbrough MZ,Unger ER,Dillner J,Zhou T

更新日期：2012-05-01 00:00:00

abstract：:The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

更新日期：1986-11-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

journal_title：Journal of clinical microbiology

authors： Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

更新日期：2014-05-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...

journal_title：Journal of clinical microbiology

authors： Humphries RM,Abbott AN,Hindler JA

更新日期：2019-05-24 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:Quantitation of hepatitis C virus (HCV) RNA in plasma and serum samples is a costly procedure in both time and reagents. Additionally, cell-associated viral RNA may not be detected. This study evaluated the accuracy of HCV RNA quantitation in small-volume whole-blood (WB) samples, which would be appropriate for point-...

journal_title：Journal of clinical microbiology

authors： Bruns T,Steinmetzer K,Ermantraut E,Stallmach A

更新日期：2009-10-01 00:00:00

abstract：:A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

journal_title：Journal of clinical microbiology

authors： Coonrod JD,Leach RP

更新日期：1978-08-01 00:00:00