Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains.

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

journal_title：Journal of clinical microbiology

authors： Kotilainen P

更新日期：1990-12-01 00:00:00

abstract：:"Campylobacter pyloridis" has been recently described as a gastritis-associated bacterium. We studied 20 strains. The bacteria had most of the characteristics of Campylobacter spp. strains. They were hippurate negative and tolerant to triphenyl tetrazolium chloride (at 0.4 and 1 mg/ml). They grew on all the media comm...

journal_title：Journal of clinical microbiology

authors： Megraud F,Bonnet F,Garnier M,Lamouliatte H

更新日期：1985-12-01 00:00:00

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

journal_title：Journal of clinical microbiology

authors： Anstead GM,Sutton DA,Graybill JR

更新日期：2012-04-01 00:00:00

abstract：:We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the v...

journal_title：Journal of clinical microbiology

authors： Petraitiene R,Petraitis V,Witt JR 3rd,Durkin MM,Bacher JD,Wheat LJ,Walsh TJ

更新日期：2011-12-01 00:00:00

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

更新日期：1991-01-01 00:00:00

abstract：:A rapid method for the detection of acetylneuraminyl hydrolase, EC 3.2.1.18 (sialidase or neuraminidase), was developed by using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid as substrate in a filter paper spot test. The method was compared to conventional assays that use 2'-(4-methylumbelliferyl)alpha-D-N-...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P

更新日期：1989-01-01 00:00:00

abstract：:The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

journal_title：Journal of clinical microbiology

authors： Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

更新日期：2005-06-01 00:00:00

abstract：:Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality con...

journal_title：Journal of clinical microbiology

authors： McQuiston JR,Parrenas R,Ortiz-Rivera M,Gheesling L,Brenner F,Fields PI

更新日期：2004-05-01 00:00:00

abstract：:For a total of 29 non-human immunodeficiency virus 1 cryptococcal meningitis cases, titer changes in the latex agglutination test before and after therapy were reviewed along with clinical manifestations, laboratory findings, and therapy regimens. The cryptococcal antigen titer decreased for every case after therapy a...

journal_title：Journal of clinical microbiology

authors： Lu H,Zhou Y,Yin Y,Pan X,Weng X

更新日期：2005-06-01 00:00:00

abstract：:Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...

journal_title：Journal of clinical microbiology

authors： Mathai E,John TJ,Rani M,Mathai D,Chacko N,Nath V,Cherian AM

更新日期：1995-07-01 00:00:00

abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

journal_title：Journal of clinical microbiology

authors： Kerremans JJ,Verbrugh HA,Vos MC

更新日期：2012-06-01 00:00:00

abstract：:Porphyromonas gingivalis is implicated in the etiology of chronic periodontitis. Genotyping studies suggest that genetic variability exists among P. gingivalis strains; however, the extent of variability remains unclear and regions of variability remain largely unidentified. To assess P. gingivalis strain diversity, w...

journal_title：Journal of clinical microbiology

authors： Igboin CO,Griffen AL,Leys EJ

更新日期：2009-10-01 00:00:00

abstract：:Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

更新日期：2007-06-01 00:00:00

abstract：:We report the first documented case of canine strangles due to infection with Streptococcus equi in a dog with enlarged lymph nodes. Genetic typing, via sequencing of 12 housekeeping genes and the SeM gene, demonstrated the isolate to be a member of a common equine strain type circulating in the United Kingdom. ...

journal_title：Journal of clinical microbiology

authors： Ladlow J,Scase T,Waller A

更新日期：2006-07-01 00:00:00

abstract：:We recently reported an outbreak of invasive aspergillosis in the major heart surgery unit of Hospital Gregorio Marañón, Madrid, Spain (T. Peláez, P. Muñoz, J. Guinea, M. Valerio, M. Giannella, C. H. W. Klaassen, and E. Bouza, Clin. Infect. Dis., in press). Aspergillus fumigatus was isolated from clinical samples from...

journal_title：Journal of clinical microbiology

authors： Guinea J,García de Viedma D,Peláez T,Escribano P,Muñoz P,Meis JF,Klaassen CH,Bouza E

更新日期：2011-10-01 00:00:00

abstract：:A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analys...

journal_title：Journal of clinical microbiology

authors： Adékambi T,Stein A,Carvajal J,Raoult D,Drancourt M

更新日期：2006-04-01 00:00:00

abstract：:From June to October of 2002, a cluster of Escherichia coli isolates producing extended-spectrum beta-lactamases (ESBLs) was detected in Stockholm. The isolates were grouped into two clones, one of which had already circulated in the same area before the outbreak. CTX-M-type ESBLs and coresistance to ciprofloxacin wer...

journal_title：Journal of clinical microbiology

authors： Fang H,Lundberg C,Olsson-Liljequist B,Hedin G,Lindbäck E,Rosenberg A,Struwe J

更新日期：2004-12-01 00:00:00

abstract：:Two species of Bartonella, a novel Bartonella clarridgeiae-like bacterium and B. vinsonii subsp. berkhoffii, were isolated from rural dogs and gray foxes in northern California. A novel B. clarridgeiae-like species was isolated from 3 (1.7%) of 182 dogs and 22 (42%) of 53 gray foxes, while B. vinsonii subsp. berkhoffi...

journal_title：Journal of clinical microbiology

authors： Henn JB,Gabriel MW,Kasten RW,Brown RN,Theis JH,Foley JE,Chomel BB

更新日期：2007-08-01 00:00:00

abstract：:CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

journal_title：Journal of clinical microbiology

authors： Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

更新日期：2013-04-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:Two cases of primary invasive cutaneous infections caused by the zoophilic dermatophytic species Microsporum canis are presented. The first case occurred in a liver transplant recipient who was receiving immunosuppressive therapy. Multiple erythematous papules were seen on both legs, and a biopsy revealed invasive fun...

journal_title：Journal of clinical microbiology

authors： King D,Cheever LW,Hood A,Horn TD,Rinaldi MG,Merz WG

更新日期：1996-02-01 00:00:00

abstract：:A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% ...

journal_title：Journal of clinical microbiology

authors： Vinayak VK,Purnima,Singh K,Venkatwswarlu K,Nain CK,Mehta SK

更新日期：1986-06-01 00:00:00

abstract：:We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...

journal_title：Journal of clinical microbiology

authors： Waites KB,Canupp KC

更新日期：2001-12-01 00:00:00

abstract：:Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

journal_title：Journal of clinical microbiology

authors： Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

更新日期：2000-06-01 00:00:00

abstract：:A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted...

journal_title：Journal of clinical microbiology

authors： Mellencamp MW,Pfeiffer NE,Suiter BT,Harness JR,Beckenhauer WH

更新日期：1989-10-01 00:00:00