Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.


:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combined with culture. Herpchek alone was not sensitive enough, but in combination with culture, maximum detection was achieved.


J Clin Microbiol


Verano L,Michalski FJ




Has Abstract


1995-05-01 00:00:00












  • Monitoring treatment of patients with pulmonary tuberculosis: can PCR be applied?

    abstract::To assess whether PCR is applicable for monitoring the efficacy of antituberculous treatment, respiratory specimens obtained during treatment and follow-up from sputum smear-positive tuberculosis (TB) patients were examined. First, results of smear, culture, and PCR for Mycobacterium tuberculosis complex (MTB) and an ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Thomsen VO,Kok-Jensen A,Buser M,Philippi-Schulz S,Burkardt HJ

    更新日期:1999-11-01 00:00:00

  • Analysis of immunoglobulin G antibody responses after administration of live and inactivated influenza A vaccine indicates that nasal wash immunoglobulin G is a transudate from serum.

    abstract::Following intranasal administration of live influenza A virus vaccine or parenteral inoculation of inactivated influenza virus vaccine, immunoglobulin antibody to the influenza virus hemagglutinin was detected in nasal wash specimens from adult volunteers. Several observations supported the suggestion that this immuno...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wagner DK,Clements ML,Reimer CB,Snyder M,Nelson DL,Murphy BR

    更新日期:1987-03-01 00:00:00

  • Evaluation of Murex CMV DNA hybrid capture assay for detection and quantitation of cytomegalovirus infection in patients following allogeneic stem cell transplantation.

    abstract::Murex hybrid capture DNA assay (HCS) is a solution hybridization antibody capture assay for detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. To determine whether CMV HCS is sensitive enough to initiate and monitor antiviral therapy after allogeneic stem cell transplantation (SCT), 51 consecutive ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hebart H,Gamer D,Loeffler J,Mueller C,Sinzger C,Jahn G,Bader P,Klingebiel T,Kanz L,Einsele H

    更新日期:1998-05-01 00:00:00

  • Sequencing and sequence analysis of VP7 and NSP5 genes reveal emergence of a new genotype of bovine group B rotaviruses in India.

    abstract::Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Barman P,Ghosh S,Das S,Varghese V,Chaudhuri S,Sarkar S,Krishnan T,Bhattacharya SK,Chakrabarti A,Kobayashi N,Naik TN

    更新日期:2004-06-01 00:00:00

  • Molecular phylogeny and proposal of two new species of the emerging pathogenic fungus Saksenaea.

    abstract::Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Alvarez E,Garcia-Hermoso D,Sutton DA,Cano JF,Stchigel AM,Hoinard D,Fothergill AW,Rinaldi MG,Dromer F,Guarro J

    更新日期:2010-12-01 00:00:00

  • Earlier positivity of central-venous- versus peripheral-blood cultures is highly predictive of catheter-related sepsis.

    abstract::To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively per...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Blot F,Schmidt E,Nitenberg G,Tancrède C,Leclercq B,Laplanche A,Andremont A

    更新日期:1998-01-01 00:00:00

  • High rate of tetracycline resistance in Streptococcus pyogenes in Iran: an epidemiological study.

    abstract::Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibioti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jasir A,Tanna A,Noorani A,Mirsalehian A,Efstratiou A,Schalen C

    更新日期:2000-06-01 00:00:00

  • Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag.

    abstract::To provide more sensitive and convenient methods for the detection of equine infectious anemia virus (EIAV), we developed an enzyme-linked immunosorbent assay (ELISA) employing the EIAV gag precursor (Pr55gag) produced by using recombinant DNA techniques. The antigenic reactivity of the recombinant EIAV Pr55gag was fo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Archambault D,Wang ZM,Lacal JC,Gazit A,Yaniv A,Dahlberg JE,Tronick SR

    更新日期:1989-06-01 00:00:00

  • Capillary gas chromatographic analysis of carbohydrates of Legionella pneumophila and other members of the family Legionellaceae.

    abstract::Legionella pneumophila, the causative agent of Legionnaires disease, and related organisms have previously been characterized primarily by conventional bacteriological methods, DNA-DNA hybridization, antigenic analysis, and fatty acid analysis. By capillary gas chromatographic analysis for carbohydrates, we have shown...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fox A,Lau PY,Brown A,Morgan SL,Zhu ZT,Lema M,Walla MD

    更新日期:1984-03-01 00:00:00

  • Comparison of BACTEC PLUS blood culture media to BacT/Alert FA blood culture media for detection of bacterial pathogens in samples containing therapeutic levels of antibiotics.

    abstract::Blood culture bottles with antimicrobial removal systems are recommended for patients who develop fever while on antibiotics. This study compared the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Alert FA bottles to effectively remove vancomycin, cefoxitin, ceftriaxone, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Flayhart D,Borek AP,Wakefield T,Dick J,Carroll KC

    更新日期:2007-03-01 00:00:00

  • Blinded comparison of a direct immunofluorescent monoclonal antibody staining method and a Giemsa staining method for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens of patients infected with human immunodefic

    abstract::A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wolfson JS,Waldron MA,Sierra LS

    更新日期:1990-09-01 00:00:00

  • Detection and Prevalence of Penicillin-Susceptible Staphylococcus aureus in the United States in 2013.

    abstract::Using blaZ PCR as the "gold standard," the sensitivities of CLSI penicillin zone edge and nitrocefin-based tests for β-lactamase production in Staphylococcus aureus were 64.5% and 35.5%, respectively, with specificity of 99.8% for both methods. In 2013, 13.5% of 3,083 S. aureus isolates from 31 U.S. centers were penic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Richter SS,Doern GV,Heilmann KP,Miner S,Tendolkar S,Riahi F,Diekema DJ

    更新日期:2016-03-01 00:00:00

  • Development of a TT virus DNA quantification system using real-time detection PCR.

    abstract::Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of the healthy population is infected with TTV. To elucidate the differences between TTV infection in patients with liver diseases and TTV infection in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kato T,Mizokami M,Mukaide M,Orito E,Ohno T,Nakano T,Tanaka Y,Kato H,Sugauchi F,Ueda R,Hirashima N,Shimamatsu K,Kage M,Kojiro M

    更新日期:2000-01-01 00:00:00

  • Use of the BacT/alert system for rapid detection of microbial contamination in a pilot study using pancreatic islet cell products.

    abstract::At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Murray L,McGowan N,Fleming J,Bailey L

    更新日期:2014-10-01 00:00:00

  • Immunoelectrophoresis for detection of polysaccharides in immune complexes.

    abstract::A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Coonrod JD,Leach RP

    更新日期:1978-08-01 00:00:00

  • Identification of pseudorabies virus-exposed swine with a gI glycoprotein enzyme-linked immunosorbent assay.

    abstract::A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mellencamp MW,Pfeiffer NE,Suiter BT,Harness JR,Beckenhauer WH

    更新日期:1989-10-01 00:00:00

  • Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis.

    abstract::Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

    更新日期:1996-08-01 00:00:00

  • Evaluation of a new reagent for anti-cytomegalovirus and anti-Epstein-Barr virus immunoglobulin G.

    abstract::The Enzygnost alpha method was tested against the complement fixation test and anti-VCA immunofluorescence to determine the respective titers of anti-cytomegalovirus and anti-Epstein-Barr virus immunoglobulin G antibodies. For cytomegalovirus, the Enzygnost results showed 97.99% agreement with the readings obtained by...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gutierrez J,Maroto MD,Piédrola G

    更新日期:1994-10-01 00:00:00

  • Comparative analysis of agr locus diversification and overall genetic variability among bovine and human Staphylococcus aureus isolates.

    abstract::The accessory gene regulator (agr) is a central system that controls the expression of Staphylococcus aureus virulence factors. In this study, the distribution of agr alleles, defined by agr restriction fragment length polymorphism within agr interference groups, among S. aureus isolates from bovine and human origin w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gilot P,van Leeuwen W

    更新日期:2004-03-01 00:00:00

  • Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real-Time PCR.

    abstract::Malaria is one of the leading causes of infectious disease in travelers returning from the tropics. The diagnosis of malaria is typically performed by examining Giemsa-stained thick and thin peripheral blood smears, which is time consuming, labor intensive, and requires high levels of proficiency. Alternatively, loop-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rypien C,Chow B,Chan WW,Church DL,Pillai DR

    更新日期:2017-10-01 00:00:00

  • Murine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A.

    abstract::To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Luk MC,Tsang RS,Ng MH

    更新日期:1987-11-01 00:00:00

  • Prevalence of Taiwan variant of Epstein-Barr virus in throat washings from patients with head and neck tumors in Taiwan.

    abstract::The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

    更新日期:1994-01-01 00:00:00

  • Unreliable extended-spectrum beta-lactamase detection in the presence of plasmid-mediated AmpC in Escherichia coli clinical isolates.

    abstract::The emergence of extended-spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC (pAmpC) enzymes in Escherichia coli raises concern regarding accurate laboratory detection and interpretation of susceptibility testing results. Twenty-six cefpodoxime ESBL screen-positive, cefoxitin-resistant E. coli clinical isolates ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Robberts FJ,Kohner PC,Patel R

    更新日期:2009-02-01 00:00:00

  • Homogeneous amplification of genital human alpha papillomaviruses by PCR using novel broad-spectrum GP5+ and GP6+ primers.

    abstract::Human papillomavirus (HPV) DNA detection and typing are important for diagnosis and management of HPV-associated diseases. One of the most commonly used PCR methods, GP5+/6+, shows weaknesses in amplifying certain types. To circumvent this limitation, we developed and validated broad-spectrum primers targeting the GP5...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schmitt M,Dondog B,Waterboer T,Pawlita M

    更新日期:2008-03-01 00:00:00

  • Breast abscess associated with Helcococcus kunzii.

    abstract::Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chagla AH,Borczyk AA,Facklam RR,Lovgren M

    更新日期:1998-08-01 00:00:00

  • Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification.

    abstract::A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and si...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhou X,Kong F,Sorrell TC,Wang H,Duan Y,Chen SC

    更新日期:2008-07-01 00:00:00

  • HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci.

    abstract::A set of universal degenerate primers which amplified, by PCR, a 600-bp oligomer encoding a portion of the 60-kDa heat shock protein (HSP60) of both Staphylococcus aureus and Staphylococcus epidermidis were developed. However, when used as a DNA probe, the 600-bp PCR product generated from S. epidermidis failed to cro...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Goh SH,Potter S,Wood JO,Hemmingsen SM,Reynolds RP,Chow AW

    更新日期:1996-04-01 00:00:00

  • Sonication of explanted prosthetic components in bags for diagnosis of prosthetic joint infection is associated with risk of contamination.

    abstract::Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

    更新日期:2006-02-01 00:00:00

  • High metabolic potential may contribute to the success of ST131 uropathogenic Escherichia coli.

    abstract::Uropathogenic Escherichia coli (UPEC) is the predominant cause of urinary tract infection in both hospital and community settings. The recent emergence of multidrug-resistant clones like the O25b:H4-ST131 lineage represents a significant threat to health, and numerous studies have explored the virulence potential of t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gibreel TM,Dodgson AR,Cheesbrough J,Bolton FJ,Fox AJ,Upton M

    更新日期:2012-10-01 00:00:00

  • Diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin G antibodies.

    abstract::Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with Entamoeba histolytica or Entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of E. histolytica galactose-inhibitable lectin antigen and salivary immunog...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Abd-Alla MD,Jackson TF,Reddy S,Ravdin JI

    更新日期:2000-06-01 00:00:00