Staphylococcal exanthematous disease in a newborn due to a virulent methicillin-resistant Staphylococcus aureus strain containing the TSST-1 gene in Europe: an alert for neonatologists.

abstract：:Rotavirus G and P types from 716 children with acute diarrhea in Dijon from 1995 to 1998 and throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed by P[8],G4, which emerged during the last winter. G9 and P[6] strains were detected at low frequencies...

journal_title：Journal of clinical microbiology

authors： Bon F,Fromantin C,Aho S,Pothier P,Kohli E

更新日期：2000-04-01 00:00:00

abstract：:Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was pos...

journal_title：Journal of clinical microbiology

authors： Díaz R,Maraví-Poma E,Delgado G,Rivero A

更新日期：1978-02-01 00:00:00

abstract：:The main goal of this study was to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), particularly livestock-associated MRSA (LA-MRSA) in pigs and pork. The genotypic relatedness of isolates on the farm, at slaughter, and at the retail level was assessed. Paired nasal and perianal swab sa...

journal_title：Journal of clinical microbiology

authors： Molla B,Byrne M,Abley M,Mathews J,Jackson CR,Fedorka-Cray P,Sreevatsan S,Wang P,Gebreyes WA

更新日期：2012-11-01 00:00:00

abstract：:The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

journal_title：Journal of clinical microbiology

authors： Garrett MA,Holmes HT,Nolte FS

更新日期：1992-07-01 00:00:00

abstract：:In order to increase the database for in vitro growth and/or susceptibility testing in liquid media, we evaluated the growth of Helicobacter pylori in broth media containing 5% sheep blood. We also compared the effect of bismuth on the growth of H. pylori in broth media containing 10% fetal calf serum with the effect ...

journal_title：Journal of clinical microbiology

authors： Coudron PE,Stratton CW

更新日期：1995-04-01 00:00:00

abstract：:This study aimed to assess the interlaboratory reproducibility at four university hospital laboratories in the southeast region of France of the Etest technique for the determination of caspofungin (CAS) and amphotericin B (AMB) MICs and to compare it to the CLSI broth microdilution reference method. Consecutive clini...

journal_title：Journal of clinical microbiology

authors： Ranque S,Lachaud L,Gari-Toussaint M,Michel-Nguyen A,Mallié M,Gaudart J,Bertout S

更新日期：2012-07-01 00:00:00

abstract：:The amino acids L-arginine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine, and L-valine were essential for the growth of Legionella pneumophila in a chemically defined medium. A partial requirement for L-cysteine (or L-cystine) was also observed. A minimal medium containing only the eight required amino...

journal_title：Journal of clinical microbiology

authors： Tesh MJ,Miller RD

更新日期：1981-05-01 00:00:00

abstract：:Malaria is one of the leading causes of infectious disease in travelers returning from the tropics. The diagnosis of malaria is typically performed by examining Giemsa-stained thick and thin peripheral blood smears, which is time consuming, labor intensive, and requires high levels of proficiency. Alternatively, loop-...

journal_title：Journal of clinical microbiology

authors： Rypien C,Chow B,Chan WW,Church DL,Pillai DR

更新日期：2017-10-01 00:00:00

abstract：:There are no previous reports of human infection due to Acinetobacter baylyi. In this study, we report on six patients with bacteremia due to A. baylyi, based on analysis of the 16S-23S rRNA intergenic spacer and the 16S rRNA gene. All six patients had multiple underlying diseases. The infection was nosocomially acqui...

journal_title：Journal of clinical microbiology

authors： Chen TL,Siu LK,Lee YT,Chen CP,Huang LY,Wu RC,Cho WL,Fung CP

更新日期：2008-09-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes....

journal_title：Journal of clinical microbiology

authors： Ajayi T,Allmond LR,Sawa T,Wiener-Kronish JP

更新日期：2003-08-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to Oc...

journal_title：Journal of clinical microbiology

authors： Chatzigeorgiou KS,Sergentanis TN,Tsiodras S,Hamodrakas SJ,Bagos PG

更新日期：2011-09-01 00:00:00

abstract：:The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

journal_title：Journal of clinical microbiology

authors： Helweg-Larsen J,Lundgren B,Lundgren JD

更新日期：2001-10-01 00:00:00

abstract：:Eight Staphylococcus aureus strains initially identified by Vitek GPS-BS or GPS-SA cards as resistant to oxacillin, but susceptible to most non-beta-lactam antibiotics, were found on further testing to be susceptible to oxacillin and ceftizoxime by disk diffusion tests. For all these strains, the MICs of oxacillin wer...

journal_title：Journal of clinical microbiology

authors： Ribeiro J,Vieira FD,King T,D'Arezzo JB,Boyce JM

更新日期：1999-05-01 00:00:00

abstract：:A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus (HRSV). Detection of each viral product and of an internal control was based on determination of specific melting temperatures by the LightCycler software. Th...

journal_title：Journal of clinical microbiology

authors： Boivin G,Côté S,Déry P,De Serres G,Bergeron MG

更新日期：2004-01-01 00:00:00

abstract：:Used peritoneal dialysis fluid was collected from patients undergoing continuous ambulatory peritoneal dialysis, and its pH and composition were assessed after incubation in either air or air with 5% CO2. Precipitation of calcium, magnesium, phosphate, and proteins occurred in the dialysis fluid incubated in air at 37...

journal_title：Journal of clinical microbiology

authors： Wilcox MH,Smith DG,Evans JA,Denyer SP,Finch RG,Williams P

更新日期：1990-10-01 00:00:00

abstract：:A young immunocompetent patient was admitted for a febrile illness with malaise, arthralgias, painful leg swelling, and polyserositis. Shortly prior to becoming ill, the patient had traveled to the Northern African desert. The symptoms disappeared during treatment with antibiotics (doxycycline and ceftriaxone) but rec...

journal_title：Journal of clinical microbiology

authors： Iten A,Graf S,Egger M,Täuber M,Graf J

更新日期：2001-05-01 00:00:00

abstract：:A total of 238 isolates of Providencia stuartii obtained from infected patients in six Dublin hospitals were grouped by using serological and bacteriocin typing methods and tested for sensitivity to a number of antimicrobial agents. Most isolates were resistant to several of these agents. Resistance to tetracycline, r...

journal_title：Journal of clinical microbiology

authors： McHale PJ,Keane CT,Dougan G

更新日期：1981-06-01 00:00:00

abstract：:A lymph node excision was performed on a 45-year-old woman with left cervical swelling. The disorder which developed after the patient had undergone oral surgery for a severe periodontal disease failed to respond to antimicrobial chemotherapy. A mycobacterial strain subsequently identified by high-performance liquid c...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Goteri G,Nista D,Mariottini A,Mazzarelli G,Bornigia S

更新日期：2004-08-01 00:00:00

abstract：:The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluor...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Gentilomi G,La Placa M

更新日期：1986-07-01 00:00:00

abstract：:Adhesin-encoding operons (pap, sfa/foc, and afa) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. A quick and sensitive assay to identify these operons was developed by using the polymerase chain reaction (PCR). Three pairs of 25-mer primers were defined from the se...

journal_title：Journal of clinical microbiology

authors： Le Bouguenec C,Archambaud M,Labigne A

更新日期：1992-05-01 00:00:00

abstract：:Approximately 15 to 35% of patients with a first episode of Clostridium difficile-associated diarrhea relapse within 2 months. Between 1994 and 1997, strains from 93 hospitalized patients with C. difficile recurrences were fingerprinted by using both serotyping and PCR-ribotyping. The results showed that 48.4% of clin...

journal_title：Journal of clinical microbiology

authors： Barbut F,Richard A,Hamadi K,Chomette V,Burghoffer B,Petit JC

更新日期：2000-06-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

journal_title：Journal of clinical microbiology

authors： Williams BL,McCann GF,Schoenknecht FD

更新日期：1983-10-01 00:00:00

abstract：:Replica plating can be used for the detection of antibiotic resistance in normal flora. We have evaluated this application of the replica plating method by comparing it with a five-colony method. The replica plating method uses a single plate for each antibiotic, with a concentration just above that for borderline res...

journal_title：Journal of clinical microbiology

authors： Osterblad M,Leistevuo T,Huovinen P

更新日期：1995-12-01 00:00:00

abstract：:Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

journal_title：Journal of clinical microbiology

authors： Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

更新日期：1996-08-01 00:00:00

abstract：:A cytochrome b (cytb) gene quantitative PCR (qPCR) assay was developed to diagnose malaria in travelers. First, manual and automated DNA extractions were compared and automated DNA extraction of 400 μl of blood was found to be more efficient. Sensitivity was estimated using the WHO international standard for Plasmodiu...

journal_title：Journal of clinical microbiology

authors： Farrugia C,Cabaret O,Botterel F,Bories C,Foulet F,Costa JM,Bretagne S

更新日期：2011-06-01 00:00:00

abstract：:This report describes the results of an 11-laboratory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mecA in staphylococci. Using breakpoints of < or = 4 microg/ml for mecA-negative and > or = 6 or 8 microg/ml for mecA-positive isolates, sensitivity and specificity based o...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Brasso WB,Ferraro MJ,Hardy DJ,Knapp CC,Lonsway D,McAllister S,Reller LB,Sader HS,Shortridge D,Skov R,Weinstein MP,Zimmer BL,Patel JB

更新日期：2009-06-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00