Factors affecting growth and susceptibility testing of Helicobacter pylori in liquid media.

abstract：:Nonfermenting coryneform bacteria identified as Brevibacterium spp. were isolated from routine clinical specimens. Four strains were derived from peritoneal fluid and has presumably been involved in the pathogenesis of continuous ambulatory peritoneal dialysis peritonitis. Another five isolates most probably represent...

journal_title：Journal of clinical microbiology

authors： Gruner E,Pfyffer GE,von Graevenitz A

更新日期：1993-06-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:Several antimicrobial agents used in selective media for the isolation of Arcobacter were found to be inhibitory to strains belonging to this genus. All three species tested were susceptible to colistin and rifampin at concentrations used in selective media. Arcobacter skirrowii was the most susceptible species. 5-Flu...

journal_title：Journal of clinical microbiology

authors： Houf K,Devriese LA,De Zutter L,Van Hoof J,Vandamme P

更新日期：2001-04-01 00:00:00

abstract：:Resistance to extended-spectrum β-lactam antibiotics has led to a greater reliance upon carbapenems, but the expression of carbapenemases threatens to limit the utility of these drugs. Current methods to detect carbapenemase activity are suboptimal, requiring prolonged incubations during which ineffective therapy may ...

journal_title：Journal of clinical microbiology

authors： Kulkarni MV,Zurita AN,Pyka JS,Murray TS,Hodsdon ME,Peaper DR

更新日期：2014-07-01 00:00:00

abstract：:We describe the first fatal evolution of cellulitis due to Neisseria meningitidis serogroup Y involving an 85-year-old woman. She presented with an extensive cellulitis of the left side of the face, neck, and thorax and septic shock. In spite of active antibiotic therapy, evolution was rapidly fatal. ...

journal_title：Journal of clinical microbiology

authors： Cartolano GL,Barbier C,Arnoult L,Simon D,Ricome JL,Hayon J

更新日期：2003-08-01 00:00:00

abstract：:A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

journal_title：Journal of clinical microbiology

authors： Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

更新日期：1996-06-01 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:Microfiltration has become a popular procedure for the concentration and enumeration of bacteria. We developed a rapid and sensitive method for the differentiation of gram-positive and gram-negative bacteria, utilizing a polycarbonate membrane filter, crystal violet, iodine, 95% ethanol, and 6% carbol fuchsin, that ca...

journal_title：Journal of clinical microbiology

authors： Romero S,Schell RF,Pennell DR

更新日期：1988-07-01 00:00:00

abstract：:The value of performing routine aerobic subcultures of both vented and unvented blood culture bottles has not been evaluated critically. We studied 4,954 pairs of blood culture bottles consisting of one vented biphasic tryptic soy broth bottle (Roche Diagnostics) and one unvented Thiol broth bottle (Difco Laboratories...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Westfall LM,Murray PR

更新日期：1983-04-01 00:00:00

abstract：:Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

journal_title：Journal of clinical microbiology

authors： Landman D,Salamera J,Quale J

更新日期：2013-12-01 00:00:00

abstract：:The hydrolysis of esculin in the presence of bile has been utilized for many years for the identification of bacteria. It is especially useful in differentiating species of the genus Streptococcus. The procedure is a two-step one. First, the bacterium must grow in a particular concentration of bile, and second, it mus...

journal_title：Journal of clinical microbiology

authors： Edberg SC,Trepeta RW,Kontnick CM,Torres AR

更新日期：1985-03-01 00:00:00

abstract：:The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

journal_title：Journal of clinical microbiology

authors： Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

更新日期：2007-06-01 00:00:00

abstract：:A serum immunoglobulin G enzyme immunoassay (EIA) for Helicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of H. pylori disease in children. The reference method used was either the (13)C-urea breath test or a biopsy culture of ...

journal_title：Journal of clinical microbiology

authors： Sunnerstam B,Kjerstadius T,Jansson L,Giesecke J,Bergström M,Ejderhamn J

更新日期：1999-10-01 00:00:00

abstract：:During a 2-month period, 62 strains of Haemophilus ducreyi were isolated from 168 genital lesions and 2 lymph node aspirates. Of these strains, 22 were found on both chocolate agar and fetal bovine serum agar supplemented with vancomycin, 29 were found only on chocolate agar, and 9 were found only on fetal bovine seru...

journal_title：Journal of clinical microbiology

authors： Hannah P,Greenwood JR

更新日期：1982-11-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms...

journal_title：Journal of clinical microbiology

authors： Van TT,Mongkolrattanothai K,Arevalo M,Lustestica M,Dien Bard J

更新日期：2017-05-01 00:00:00

abstract：:A multiwell, dried antimicrobial agent susceptibility test system, Sceptor (BBL Microbiology Systems, Cockeysville, Md.), was tested. The system was compared directly with a reference microdilution method by using two collections of stock cultures and 305 fresh clinical isolates. Sceptor was found to be in agreement (...

journal_title：Journal of clinical microbiology

authors： Jones RN,Thornsberry C,Barry AL,Gavan TL

更新日期：1981-01-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:Sequence analysis of the internal transcribed spacer (ITS) region was employed as the gold standard method for yeast identification in the China Hospital Invasive Fungal Surveillance Net (CHIF-NET). It has subsequently been found that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-...

journal_title：Journal of clinical microbiology

authors： Zhang L,Xiao M,Wang H,Gao R,Fan X,Brown M,Gray TJ,Kong F,Xu YC

更新日期：2014-02-01 00:00:00

abstract：:The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,McAdam AJ

更新日期：2015-10-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacter jejuni (16 strains) from Campylobacter coli (10 strains). This observation was confirmed by the study of DNA homology obtained by a quantitative filter hybridization method. The hippurate hydrolysis tes...

journal_title：Journal of clinical microbiology

authors： Derclaye I,Delor I,Van Bouchaute M,Moureau P,Wauters G,Cornelis GR

更新日期：1989-05-01 00:00:00

abstract：:We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...

journal_title：Journal of clinical microbiology

authors： Singh DV,Isac SR,Colwell RR

更新日期：2002-11-01 00:00:00

abstract：:A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...

journal_title：Journal of clinical microbiology

authors： Dasch GA

更新日期：1981-09-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:We previously demonstrated the transmission of murine cytomegalovirus to syngeneic mice and preformed monolayers of mouse embryo fibroblasts with plastic-adherent peritoneal exudate cells from infected mice as a source of macrophages. In the present studies we compared this standard feeder layer method with a reverse ...

journal_title：Journal of clinical microbiology

authors： Winkler SR,Booss J

更新日期：1980-12-01 00:00:00

abstract：:In vitro studies established that neither cloth nor disposable diapers demonstrably contributed to the growth of Escherichia coli, Proteus vulgaris, Staphylococcus aureus, or Candida albicans when urine was present as a growth medium. In a clinical study of 166 children, the microbial skin flora of children with atopi...

journal_title：Journal of clinical microbiology

authors： Keswick BH,Seymour JL,Milligan MC

更新日期：1987-02-01 00:00:00

abstract：:Salmonella enterica serovar Senftenberg is a common nontyphoidal Salmonella serotype which causes human Salmonella infections worldwide. In this study, 182 S. Senftenberg isolates, including 17 atypical non-hydrogen sulfide (H2S)-producing isolates, were detected in China from 2005 to 2011. The microbiological and gen...

journal_title：Journal of clinical microbiology

authors： Yi S,Xie J,Liu N,Li P,Xu X,Li H,Sun J,Wang J,Liang B,Yang C,Wang X,Hao R,Wang L,Wu Z,Zhang J,Wang Y,Huang L,Sun Y,Klena JD,Meng J,Qiu S,Song H

更新日期：2014-07-01 00:00:00

abstract：:In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, a...

journal_title：Journal of clinical microbiology

authors： Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel H

更新日期：2007-09-01 00:00:00