Abstract:
:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all human picornaviruses were covered. The amplicons, which represented the 5' untranslated regions of the viral RNA genomes, were identified in liquid hybridization reactions with genus-specific digoxigenin-labeled oligonucleotide probes. The sensitivity of the multiplex RT-PCR and liquid hybridization assay was 10 to 100 picornavirus genome equivalents for representatives of each picornavirus genus. The hepatitis A virus assay exhibited a sensitivity of 10 genome copies. Both the uniplex and the multiplex tests were highly specific for the target viruses. Twenty-three clinical samples, including cerebrospinal fluid, serum, and nasopharyngeal swab specimens, were used for clinical evaluation of the multiplex RT-PCR assay. The results obtained were consistent with the results of routine virus diagnostic assays. Furthermore, the assay was used to screen 68 stool specimens for the presence of parechoviruses and Aichi virus. One sample was found to contain parechovirus RNA, whereas no Aichi virus was detected. The assay described here can be applied for the efficient identification of human enteroviruses and rhinoviruses in clinical specimens and simultaneously enables the collection of information on the epidemiology and clinical outcomes of infections caused by the currently poorly known human parechoviruses and Aichi virus.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä Tdoi
10.1128/JCM.43.3.1239-1245.2005subject
Has Abstractpub_date
2005-03-01 00:00:00pages
1239-45issue
3eissn
0095-1137issn
1098-660Xpii
43/3/1239journal_volume
43pub_type
杂志文章abstract::By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cin...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.11.2940-2947.1995
更新日期:1995-11-01 00:00:00
abstract::Mutations in the 81-bp rifampin resistance-determining region (RRDR) of the rpoB gene were analyzed by DNA sequencing of 50 Mycobacterium tuberculosis clinical isolates (44 resistant and 6 sensitive) from various parts of India. Fifty-three mutations of 18 different kinds, 17 point mutations and one deletion, were obs...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.8.2987-2990.2001
更新日期:2001-08-01 00:00:00
abstract::Hepatitis C virus (HCV)-positive sera from 106 chronically infected patients which had previously been genotyped were characterized by serotyping. Genotypes were determined by a first-generation line probe assay (INNO-LiPA HCV) and by sequence analyses of the core, core-E1, and NS5B regions. HCV serotypes were determi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.7.1784-1787.1996
更新日期:1996-07-01 00:00:00
abstract::Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.10.3541-3547.2001
更新日期:2001-10-01 00:00:00
abstract::Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.2.283-286.1990
更新日期:1990-02-01 00:00:00
abstract::The etiology of the purple urine bag syndrome (PUBS), in which the urinary catheter bag of some elderly patients develops intense purple coloration, was studied. The purple was found to be a mixture of indirubin dissolved in the plastic and indigo on its surface. Six patients with PUBS were studied, and Providencia st...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.10.2152-2156.1988
更新日期:1988-10-01 00:00:00
abstract::The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00382-15
更新日期:2015-10-01 00:00:00
abstract::A pseudooutbreak of cryptococcal meningitis was caused by the use of contaminated albumin solution used in the preparation of Papanicolaou-stained slides of cerebrospinal fluid. Organisms were seen in cytocentrifuge preparations, but not in India ink preparations of cerebrospinal fluid specimens. Cryptococcal antigen ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.15.6.1141-1143.1982
更新日期:1982-06-01 00:00:00
abstract::We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.9.3161-3164.2000
更新日期:2000-09-01 00:00:00
abstract::Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.6.1099-1103.1986
更新日期:1986-06-01 00:00:00
abstract::Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01683-19
更新日期:2020-03-25 00:00:00
abstract::A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.10.4783-4786.2003
更新日期:2003-10-01 00:00:00
abstract::Reagent-impregnated filter paper replica methods are described which can be used to detect all penicillinase (beta-lactamase)-producing Neisseria gonorrhoeae (PPNG) colonies in an entire population of Neisseria gonorrhoeae growing on primary isolation plates. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.3.545-547.1983
更新日期:1983-03-01 00:00:00
abstract::A biotype analysis was performed on serologically nontypable Haemophilus influenzae isolates from the middle ear effusions and nasopharynges of 33 children with chronic otitis media with effusion (serous otitis media). Over 50% of the H. influenzae isolates from the middle ears belonged to biotype II. The incidence of...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.6.1102-1104.1984
更新日期:1984-12-01 00:00:00
abstract::The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.8.2166-2170.1995
更新日期:1995-08-01 00:00:00
abstract::Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.42.4.1596-1600.2004
更新日期:2004-04-01 00:00:00
abstract::Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.2.530-536.2000
更新日期:2000-02-01 00:00:00
abstract::Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.3.535-539.1990
更新日期:1990-03-01 00:00:00
abstract::A fully automated microparticle enzyme immunoassay (EIA), IMx Core, was developed for the detection of antibody against hepatitis B core antigen (anti-HBc). IMx Core sensitivity was less than 0.5 Paul Ehrlich Institut units per ml and was greater than that of the commercial radioimmunoassay (RIA) or EIA, Corab and Cor...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.3.611-616.1991
更新日期:1991-03-01 00:00:00
abstract::To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.8.1748-1751.1991
更新日期:1991-08-01 00:00:00
abstract::A cluster of Mycobacterium ulcerans infections has recently occurred on Phillip Island, Victoria, Australia. Previous cases of infection have generally been located around Bairnsdale in southeast Gippsland. The aim of this study was to determine the epidemiological relationship between these strains and other strains ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.9.2250-2253.1995
更新日期:1995-09-01 00:00:00
abstract::We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:10.1128/JCM.35.10.2628-2633.1997
更新日期:1997-10-01 00:00:00
abstract::Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00136-19
更新日期:2019-06-25 00:00:00
abstract::Serologic methods are an important part of a clinical laboratory's portfolio of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests and are essential to the broader response to coronavirus infectious disease 2019 (COVID-19), including epidemiological studies and vaccine development. There are currently ...
journal_title:Journal of clinical microbiology
pub_type: 评论,杂志文章
doi:10.1128/JCM.02179-20
更新日期:2020-10-21 00:00:00
abstract::Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.1.76-78.1995
更新日期:1995-01-01 00:00:00
abstract::Streptococcus pneumoniae plays an important role in causing acute exacerbations in patients with chronic respiratory disease. However, few data are available regarding pneumococcal persistence in adult patients with chronic respiratory diseases. Fifty pneumococci recovered from sputum samples (1995 to 2010) from 13 ad...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02056-12
更新日期:2012-12-01 00:00:00
abstract::This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01935-14
更新日期:2015-01-01 00:00:00
abstract::Restriction endonuclease analysis of virulence plasmid DNA was used to study the epidemiology of Yersinia pseudotuberculosis infections. The origin of Y. pseudotuberculosis could be divided into two focus areas: Eastern Asia and Europe. Wild animals were an important reservoir for the Y. pseudotuberculosis seen in inf...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.5.1410-1413.1994
更新日期:1994-05-01 00:00:00
abstract::Studies on the molecular epidemiologic characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains have demonstrated their genetic and geographical diversity. In addition, it has been reported that there are genetic differences between community-associated (CA) and health care-associated (HA) MRSA st...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01147-07
更新日期:2007-12-01 00:00:00
abstract::The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii sho...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.12.2270-2273.1987
更新日期:1987-12-01 00:00:00