Abstract:
:A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR test. MTB was isolated from 198 of these samples. The FQ PCR assay was sensitive (197 of 198, 99.5%) and specific (165 of 168, 98.2%); no significant difference was observed between the two PCR protocols. After DNA extraction, a final result was available within 1.5 h with the real-time PCR protocol.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Cleary TJ,Roudel G,Casillas O,Miller Ndoi
10.1128/jcm.41.10.4783-4786.2003subject
Has Abstractpub_date
2003-10-01 00:00:00pages
4783-6issue
10eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
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