Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.

Abstract:

:A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR test. MTB was isolated from 198 of these samples. The FQ PCR assay was sensitive (197 of 198, 99.5%) and specific (165 of 168, 98.2%); no significant difference was observed between the two PCR protocols. After DNA extraction, a final result was available within 1.5 h with the real-time PCR protocol.

journal_name

J Clin Microbiol

authors

Cleary TJ,Roudel G,Casillas O,Miller N

doi

10.1128/jcm.41.10.4783-4786.2003

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

4783-6

issue

10

eissn

0095-1137

issn

1098-660X

journal_volume

41

pub_type

杂志文章
  • Comparison of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay and the avidity index method for identification of recent HIV infections.

    abstract::The sensitivity and specificity of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay (BED-CEIA) were compared with those of the avidity index method to identify recent HIV infection using a panel of 148 samples (81 patients) representing durations of infe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01055-07

    authors: Loschen S,Bätzing-Feigenbaum J,Poggensee G,Cordes C,Hintsche B,Rausch M,Dupke S,Gohlke-Micknis S,Rödig J,Hamouda O,Kücherer C

    更新日期:2008-01-01 00:00:00

  • Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates by heteroduplex analysis and determination of rifamycin cross-resistance in rifampin-resistant isolates.

    abstract::Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.2.816-818.2003

    authors: Saribaş Z,Kocagöz T,Alp A,Günalp A

    更新日期:2003-02-01 00:00:00

  • Monoclonal antibody capture enzyme-linked immunosorbent assay for detection of bovine enteric coronavirus.

    abstract::Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.19.3.388-393.1984

    authors: Crouch CF,Raybould TJ,Acres SD

    更新日期:1984-03-01 00:00:00

  • Validation of Autoclave Protocols for Successful Decontamination of Category A Medical Waste Generated from Care of Patients with Serious Communicable Diseases.

    abstract::In response to the Ebola outbreak in 2014, many hospitals designated specific areas to care for patients with Ebola and other highly infectious diseases. The safe handling of category A infectious substances is a unique challenge in this environment. One solution is on-site waste treatment with a steam sterilizer or a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02161-16

    authors: Garibaldi BT,Reimers M,Ernst N,Bova G,Nowakowski E,Bukowski J,Ellis BC,Smith C,Sauer L,Dionne K,Carroll KC,Maragakis LL,Parrish NM

    更新日期:2017-02-01 00:00:00

  • Characterization of a coronavirus isolated from a diarrheic foal.

    abstract::A coronavirus was isolated from feces of a diarrheic foal and serially propagated in human rectal adenocarcinoma (HRT-18) cells. Antigenic and genomic characterizations of the virus (isolate NC99) were based on serological comparison with other avian and mammalian coronaviruses and sequence analysis of the nucleocapsi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.12.4523-4526.2000

    authors: Guy JS,Breslin JJ,Breuhaus B,Vivrette S,Smith LG

    更新日期:2000-12-01 00:00:00

  • GM1 ganglioside enzyme-linked immunosorbent assay for detection of heat-labile enterotoxin produced by human and porcine Escherichia coli strains.

    abstract::Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enteroto...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.2.298-301.1982

    authors: Gustafsson B,Möllby R

    更新日期:1982-02-01 00:00:00

  • Clinical evaluation of the Roche AMPLICOR PCR Mycobacterium tuberculosis test for detection of M. tuberculosis in respiratory specimens.

    abstract::The reliability of the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB) for the diagnosis of pulmonary tuberculosis was evaluated by testing 956 respiratory specimens from 502 patients and comparing results with results by culture and medical history. Of those 135 specimens that were culture positive for ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.5.1083-1085.1996

    authors: Bergmann JS,Woods GL

    更新日期:1996-05-01 00:00:00

  • Justification for use of a single trichrome stain as the sole means for routine detection of intestinal parasites in concentrated stool specimens.

    abstract::Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.3.835-837.1999

    authors: Kellogg JA,Elder CJ

    更新日期:1999-03-01 00:00:00

  • Sequencing and comparative analysis of flagellin genes fliC, fljB, and flpA from Salmonella.

    abstract::Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality con...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.5.1923-1932.2004

    authors: McQuiston JR,Parrenas R,Ortiz-Rivera M,Gheesling L,Brenner F,Fields PI

    更新日期:2004-05-01 00:00:00

  • The L1 major capsid protein of human papillomavirus type 16 variants affects yield of virus-like particles produced in an insect cell expression system.

    abstract::The L1 major capsid proteins of six human papillomavirus type 16 (HPV-16) strains were expressed in insect cells by using recombinant baculoviruses. Virus-like particles (VLPs) which appeared similar to empty virions were identified by electron microscopy for all HPV strains investigated. However, the yield of VLPs pr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.7.2046-2051.1998

    authors: Touze A,El Mehdaoui S,Sizaret PY,Mougin C,Muñoz N,Coursaget P

    更新日期:1998-07-01 00:00:00

  • Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.

    abstract::A Legionella-like organism (strain 798-PA-H, ATCC 35999) isolated from a lung aspirate was shown by DNA hybridization to belong to the species Legionella hackeliae. Growth, gas-liquid chromatographic, and physiologic characteristics were consistent with those of the type strain of L. hackeliae, Lansing 2 (ATCC 35250)....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.4.488-489.1985

    authors: Wilkinson HW,Thacker WL,Steigerwalt AG,Brenner DJ,Ampel NM,Wing EJ

    更新日期:1985-10-01 00:00:00

  • Multicenter evaluation of a transcription-reverse transcription concerted assay for rapid detection of mycobacterium tuberculosis complex in clinical specimens.

    abstract::A European multicenter study was performed to evaluate the performance of a new method, based on the transcription-reverse transcription concerted reaction (TRC-2), which enabled one-step amplification and real-time detection of the Mycobacterium tuberculosis 16S rRNA target directly in clinical specimens. A total of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.01730-08

    authors: Drouillon V,Delogu G,Dettori G,Lagrange PH,Benecchi M,Houriez F,Baroli K,Ardito F,Torelli R,Chezzi C,Fadda G,Herrmann JL

    更新日期:2009-11-01 00:00:00

  • Paracoccus yeeii sp. nov. (formerly CDC group EO-2), a novel bacterial species associated with human infection.

    abstract::CDC eugonic oxidizer group 2 (EO-2) is a group of unclassified gram-negative bacterial strains isolated from various human sources. As determined by biochemical tests and analyses of fatty acid compositions, these organisms form a homogeneous group that appears to be distinct from but related to other Paracoccus speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.3.1289-1294.2003

    authors: Daneshvar MI,Hollis DG,Weyant RS,Steigerwalt AG,Whitney AM,Douglas MP,Macgregor JP,Jordan JG,Mayer LW,Rassouli SM,Barchet W,Munro C,Shuttleworth L,Bernard K

    更新日期:2003-03-01 00:00:00

  • Fluorogenic assay for differentiating Staphylococcus warneri and Staphylococcus hominis strains of bovine origin.

    abstract::A fluorogenic assay for the detection of beta-glucosidase was developed as part of a simplified conventional method to distinguish Staphylococcus warneri and Staphylococcus hominis isolated from bovine body sites. The assay is based on the fact that strains of S. warneri produce beta-glucosidase, while strains of S. h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.3.602-.1990

    authors: White DG,Harmon RJ,Langlois BE

    更新日期:1990-03-01 00:00:00

  • Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay.

    abstract::To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.12.3072-3074.1996

    authors: Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

    更新日期:1996-12-01 00:00:00

  • Evaluation of a Rapid Diagnostic Test for Detection of Burkholderia pseudomallei in the Lao People's Democratic Republic.

    abstract::Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02002-17

    authors: Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

    更新日期:2018-06-25 00:00:00

  • Evaluation of the automicrobic system for susceptibility testing of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin.

    abstract::The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.19.4.502-505.1984

    authors: Woolfrey BF,Lally RT,Ederer MN,Quall CO

    更新日期:1984-04-01 00:00:00

  • Identification of a new methicillin-resistant Staphylococcus aureus strain that cannot be assigned to any known spa type.

    abstract::spa typing is a method used in molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). Due to the lack of an amplification product in one of 55 MRSA cases, a new PCR setup, which allows an indicative description of a previously unknown type, was performed. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00933-09

    authors: Reischauer M,Bernkopf M,Webersinke C

    更新日期:2010-02-01 00:00:00

  • Molecular parameters for precise diagnosis of asymptomatic Epstein-Barr virus reactivation in healthy carriers.

    abstract::Asymptomatic Epstein-Barr virus (EBV) reactivations periodically occur in oral mucosa-associated lymphoid tissues. Until now, EBV reactivation has been diagnosed by serologic profiles that suggest virus replication. Serologic responses, however, are delayed and do not necessarily indicate ongoing replicative activity....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.12.5419-5428.2003

    authors: Maurmann S,Fricke L,Wagner HJ,Schlenke P,Hennig H,Steinhoff J,Jabs WJ

    更新日期:2003-12-01 00:00:00

  • Humoral immune response to human cytomegalovirus DNA polymerase.

    abstract::In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and no...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.3.724-726.1993

    authors: Landini MP,Lazzarotto T,Ertl PF

    更新日期:1993-03-01 00:00:00

  • Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans.

    abstract::Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.14.2.135-140.1981

    authors: Wolff KL,Muth DJ,Hudson BW,Trent DW

    更新日期:1981-08-01 00:00:00

  • Escherichia coli O157:H7 and O157:H(-) strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome.

    abstract::We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H(-) isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.11.3491-3496.1999

    authors: Schmidt H,Scheef J,Huppertz HI,Frosch M,Karch H

    更新日期:1999-11-01 00:00:00

  • Evaluation of a multiplex real-time reverse transcriptase PCR assay for detection and differentiation of influenza viruses A and B during the 2001-2002 influenza season in Israel.

    abstract::The ability to rapidly diagnose influenza virus infections is of the utmost importance in the evaluation of patients with upper respiratory tract infections. It is also important for the influenza surveillance activities performed by national influenza centers. In the present study we modified a multiplex real-time re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.2.589-595.2005

    authors: Hindiyeh M,Levy V,Azar R,Varsano N,Regev L,Shalev Y,Grossman Z,Mendelson E

    更新日期:2005-02-01 00:00:00

  • Escherichia hermannii as the sole isolate from a patient with purulent conjunctivitis.

    abstract::Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01119-08

    authors: Poulou A,Dimitroulia E,Markou F,Tsakris A

    更新日期:2008-11-01 00:00:00

  • Diversity among French Bacillus anthracis isolates.

    abstract::While outbreaks of animal anthrax zoonoses still regularly occur in France, little is known about the epidemiology links between them. We have used the eight-locus multilocus variable-number tandem repeat analysis typing technique against a collection of 50 Bacillus anthracis isolates from France. There were eight dis...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4732-4734.2002

    authors: Fouet A,Smith KL,Keys C,Vaissaire J,Le Doujet C,Lévy M,Mock M,Keim P

    更新日期:2002-12-01 00:00:00

  • Identification of optimal epitopes for Plasmodium falciparum rapid diagnostic tests that target histidine-rich proteins 2 and 3.

    abstract::Rapid diagnostic tests (RDTs) represent important tools to diagnose malaria infection. To improve understanding of the variable performance of RDTs that detect the major target in Plasmodium falciparum, namely, histidine-rich protein 2 (HRP2), and to inform the design of better tests, we undertook detailed mapping of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06533-11

    authors: Lee N,Gatton ML,Pelecanos A,Bubb M,Gonzalez I,Bell D,Cheng Q,McCarthy JS

    更新日期:2012-04-01 00:00:00

  • Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal.

    abstract::Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.6.2744-2747.2003

    authors: Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

    更新日期:2003-06-01 00:00:00

  • Use of PCR-restriction enzyme pattern analysis and sequencing database for hsp65 gene-based identification of Nocardia species.

    abstract::Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.2.536-546.2006

    authors: Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

    更新日期:2006-02-01 00:00:00

  • Spurious sulfamethoxazole-trimethoprim resistance of Salmonella typhi.

    abstract::Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.1.205-206.1986

    authors: Escamilla J,Kilpatrick ME,Ugarte HF

    更新日期:1986-01-01 00:00:00

  • Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid.

    abstract::A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.11.3028-3030.1993

    authors: Katz JB,Alstad AD,Gustafson GA,Moser KM

    更新日期:1993-11-01 00:00:00