Abstract:
:The etiology of the purple urine bag syndrome (PUBS), in which the urinary catheter bag of some elderly patients develops intense purple coloration, was studied. The purple was found to be a mixture of indirubin dissolved in the plastic and indigo on its surface. Six patients with PUBS were studied, and Providencia stuartii was isolated from the urine of five and Klebsiella pneumoniae was isolated from the urine of one. These strains produced indigo in 7.9 mM indoxyl sulfate-containing agar. One hundred and fifty isolates of 41 species of bacteria were tested for their ability to produce indigo on agar containing indoxyl sulfate, but only 17 of 27 strains of P. stuartii, a single strain of Klebsiella pneumoniae, and Enterobacter agglomerans were positive. All of the indigo-producing bacteria had an indoxyl phosphatase with a pI of 6.4. This enzyme also possessed indoxyl sulfatase activity and was not present in strains that were unable to produce indigo from indoxyl sulfate. We conclude that PUBS results from the decomposition of urinary indoxyl sulfate to indigo and indirubin by bacteria (notably P. stuartii). As elderly catheterized patients often have high urinary indoxyl sulfate levels and colonization of their urinary tract with P. stuartii, the condition is most commonly seen in them.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Dealler SF,Hawkey PM,Millar MRdoi
10.1128/JCM.26.10.2152-2156.1988subject
Has Abstractpub_date
1988-10-01 00:00:00pages
2152-6issue
10eissn
0095-1137issn
1098-660Xjournal_volume
26pub_type
杂志文章abstract::DNA colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (CT) was used to screen 12 clinical isolates of Aeromonas hydrophila. Under stringent hybridizing (overnight at 40 degrees C) and washing (1 h at 50 degrees C) conditions, nine strains reacted with the 32P-labele...
journal_title:Journal of clinical microbiology
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pub_type: 杂志文章,多中心研究
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doi:10.1128/JCM.44.3.1087-1089.2006
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journal_title:Journal of clinical microbiology
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更新日期:2007-07-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.14.3.273-280.1981
更新日期:1981-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.6.1088-1090.1986
更新日期:1986-06-01 00:00:00
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更新日期:2001-05-01 00:00:00
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更新日期:1990-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.6.1592-1594.1996
更新日期:1996-06-01 00:00:00
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更新日期:2002-04-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1998-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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更新日期:2003-08-01 00:00:00
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