Detection of transcripts of human papillomaviruses 16 and 18 in cancer-derived cell lines and cervical biopsies by enzyme immunoassay for DNA-RNA hybrids following solution hybridization.

Abstract:

:mRNAs of human papillomaviruses (HPV) 16 and 18 were detected in cancer-derived cell lines and genital tract biopsy specimens by a novel hybridization assay. Biotinylated whole genomic HPV DNA probes were hybridized in solution to extracted total nucleic acids. Hybrids between the labeled probes and RNA transcripts were captured on a microplate coated with an antibiotin antibody. Bound hybrids were incubated with a beta-galactosidase-labeled monoclonal antibody to DNA-RNA hybrids and measured by the addition of a fluorogenic substrate. HPV 18 and HPV 16 mRNAs were detected in nucleic acids from 2.3 x 10(3) HeLa cells and 10(4) SiHa cells, respectively. The specificity of the assay for mRNA was demonstrated by the low reactivity of nucleic acids from SiHa cells after treatment with T1 RNase and by the selective reactivity of cellular nucleic acids which bound to an oligo(dT) column. With HPV 16 subgenomic probes, E6-E7 transcripts but not L1-L2 transcripts were detected in SiHa cells. Tests of 58 biopsy specimens from 31 patients showed that the detection of HPV 16 and HPV 18 transcripts in tissue specimens was feasible. Analysis of biopsy specimens with subgenomic probes revealed HPV 16 E6-E7 transcripts in all specimens that reacted with the whole genomic probe, while L1-L2 transcripts were found infrequently.

journal_name

J Clin Microbiol

authors

Coutlée F,Shah KV,Rader JS,Currie JL,Viscidi RP

doi

10.1128/JCM.29.5.968-974.1991

keywords:

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

968-74

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

29

pub_type

杂志文章
  • Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats.

    abstract::Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.5.1159-1165.1994

    authors: Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

    更新日期:1994-05-01 00:00:00

  • Evaluation of the immunoglobulin G avidity test for diagnosis of toxoplasmic lymphadenopathy.

    abstract::Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.10.4627-4631.2004

    authors: Montoya JG,Huffman HB,Remington JS

    更新日期:2004-10-01 00:00:00

  • Evaluation of three influenza A and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses.

    abstract::The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 30 influenza A/H3 vi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02464-09

    authors: Selvaraju SB,Selvarangan R

    更新日期:2010-11-01 00:00:00

  • Detection and quantitation of the glucuronoxylomannan-like polysaccharide antigen from clinical and nonclinical isolates of Trichosporon beigelii and implications for pathogenicity.

    abstract::Sera from patients with systemic infections caused by the opportunistic fungus Trichosporon beigelii have been shown to cross-react with anticryptococcal antibodies. We quantitatively compared the amounts of antigen produced and examined the expression of O-acetyl epitopes from 35 strains of T. beigelii isolated from ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.1.126-130.1995

    authors: Lyman CA,Devi SJ,Nathanson J,Frasch CE,Pizzo PA,Walsh TJ

    更新日期:1995-01-01 00:00:00

  • Neutralization of antimicrobial substances in new BacT/Alert FA and FN Plus blood culture bottles.

    abstract::Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00103-13

    authors: Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

    更新日期:2013-05-01 00:00:00

  • Evaluation of pooling strategies for acute HIV-1 infection screening using nucleic acid amplification testing.

    abstract::A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00650-11

    authors: Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

    更新日期:2011-10-01 00:00:00

  • Detection of Toxoplasma gondii by PCR and tissue culture in cerebrospinal fluid and blood of human immunodeficiency virus-seropositive patients.

    abstract::To investigate whether both tissue culture and PCR on a sequence from the repetitive rDNA could contribute to the diagnosis of toxoplasmosis, blood samples and, if they were available, cerebrospinal fluid (CSF) and aqueous humor samples from 72 human immunodeficiency virus-seropositive patients with suspected toxoplas...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.9.2421-2426.1995

    authors: Dupon M,Cazenave J,Pellegrin JL,Ragnaud JM,Cheyrou A,Fischer I,Leng B,Lacut JY

    更新日期:1995-09-01 00:00:00

  • Characterization of some actinomyces-like isolates from human clinical sources: description of Varibaculum cambriensis gen nov, sp nov.

    abstract::Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.2.640-644.2003

    authors: Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

    更新日期:2003-02-01 00:00:00

  • Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.

    abstract::Growth curves of the yeast form of Paracoccidioides brasiliensis B-339 based on total and viable cell counts were determined. Crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. Different patterns of proteins were obtained by affinity chromatography on Sepharose 4B-immunog...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.10.2147-2151.1988

    authors: De Camargo Z,Unterkircher C,Campoy SP,Travassos LR

    更新日期:1988-10-01 00:00:00

  • Ovine-associated Staphylococcus aureus protein with immunochemical similarity to toxic shock syndrome toxin 1.

    abstract::A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.1.210-212.1989

    authors: Ho G,Campbell WH,Carlson E

    更新日期:1989-01-01 00:00:00

  • Evaluation of Sensitivity and Specificity Performance of Elecsys HTLV-I/II Assay in a Multicenter Study in Europe and Japan.

    abstract::Screening of blood for human T-cell lymphotropic virus type 1 and type 2 (HTLV-1 and -2, respectively) is important to diagnose and prevent infection and ensure the safety of blood supplies. The Elecsys HTLV-I/II assay is a newly developed, electrochemiluminescence screening assay for the detection of HTLV-1/2 infecti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00169-17

    authors: Laperche S,Sauleda S,Piron M,Mühlbacher A,Schennach H,Schottstedt V,Queirós L,Uno N,Yanagihara K,Imdahl R,Hey A,Klinkicht M,Melchior W,Muench P,Watanabe T

    更新日期:2017-07-01 00:00:00

  • Hantavirus disease outbreak in Germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin.

    abstract::In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02573-06

    authors: Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel H

    更新日期:2007-09-01 00:00:00

  • Comparative evaluation of nonradiometric BACTEC and improved oxoid signal blood culture systems in a clinical laboratory.

    abstract::The BACTEC NR660 blood culture system, which uses infrared spectroscopy to detect carbon dioxide generated by bacterial growth, was compared with the new medium formulation of the Oxoid Signal system. Two trials were conducted: a comparative study of 88 organisms in simulated blood cultures and a clinical trial of 3,3...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.7.1586-1590.1990

    authors: Daley C,Lim I,Modra J,Wilkinson I

    更新日期:1990-07-01 00:00:00

  • Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strains.

    abstract::By use of the nearly perfectly colinear genomes of Rickettsia conorii and Rickettsia prowazekii, we compared the usefulness of three types of sequences for typing of R. conorii isolates: (i) 5 variable coding genes comprising the 16S ribosomal DNA, gltA, ompB, and sca4 (gene D) genes, which are present in both genomes...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.12.5757-5766.2004

    authors: Fournier PE,Zhu Y,Ogata H,Raoult D

    更新日期:2004-12-01 00:00:00

  • Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein.

    abstract::A glycoprotein from bovine erythrocyte membrane was evaluated in two immunoassays as a reagent for the serodiagnosis of infectious mononucleosis (IM). We previously reported that a partially purified preparation of this glycoprotein, when attached to latex beads, agglutinated in the presence of IM heterophile antibody...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.3.495-499.1983

    authors: Fletcher MA,Klimas NG,Latif ZA,Caldwell KE

    更新日期:1983-09-01 00:00:00

  • Introduction of a new hepatitis agent in retrospect: genetic studies of Swedish hepatitis D virus strains.

    abstract::The supposedly first outbreak of hepatitis D virus (HDV) infection in Sweden occurred among intravenous drug addicts in the Malmö area in the mid-1970s. Stored sera from this outbreak were used for viral RNA extraction and analysis. By sequence comparisons, the HDV genomes from those Swedish patients fell into two sep...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.11.2713-2717.1996

    authors: Zhang YY,Hansson BG

    更新日期:1996-11-01 00:00:00

  • Infectivity of human coronavirus strain 229E.

    abstract::The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.3.462-468.1980

    authors: Macnaughton MR,Thomas BJ,Davies HA,Patterson S

    更新日期:1980-09-01 00:00:00

  • Validity of cultures of fluid collected through drainage catheters versus those obtained by direct aspiration.

    abstract::To examine the validity of cultures of fluid collected through drainage catheters, we reviewed retrospectively fluid specimens that had been collected through catheters in place for at least 2 days. These specimens were taken from patients at a large tertiary-care hospital. A total of 974 specimens representing 620 pa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.1.66-68.2001

    authors: Everts RJ,Heneghan JP,Adholla PO,Reller LB

    更新日期:2001-01-01 00:00:00

  • Detection of the 70-kilodalton histoplasma capsulatum antigen in serum of histoplasmosis patients: correlation between antigenemia and therapy during follow-up.

    abstract::Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals, who may develop a progressive disseminated form which is often fatal if it is untreated. In such patients, the detection of antibody responses for both diagnosis and follow-up may be of limited use, whereas the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.3.675-680.1999

    authors: Gómez BL,Figueroa JI,Hamilton AJ,Diez S,Rojas M,Tobón A,Restrepo A,Hay RJ

    更新日期:1999-03-01 00:00:00

  • Comparison of drug sensitivity testing with microdilution quantitative minimum inhibitory concentration and the Autobac I system.

    abstract::Antibiotic susceptibility studies were compared by the quantitative minimum inhibitory concentration and Autobac I. Four hundred common isolates from clinical materials including both gram-negative and -positive organisms were evaluated. Results demonstrate that the Autobac I method can provide reliable semiquantitati...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.5.465-469.1980

    authors: Jacob CV,Kleineman J

    更新日期:1980-05-01 00:00:00

  • Recurrent breast abscesses caused by Corynebacterium minutissimum.

    abstract::A 42-year-old woman developed severe, recurrent breast abscesses caused by Corynebacterium minutissimum. Prior reports of C. minutissimum infection have been limited to erythrasma, a minor dermatosis. The microbiological and clinical features of this species were reviewed. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.6.1219-1220.1984

    authors: Berger SA,Gorea A,Stadler J,Dan M,Zilberman M

    更新日期:1984-12-01 00:00:00

  • Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools.

    abstract::A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.11.5134-5146.2003

    authors: Fukushima H,Tsunomori Y,Seki R

    更新日期:2003-11-01 00:00:00

  • Comparing QuantiFERON-TB Gold Plus with Other Tests To Diagnose Mycobacterium tuberculosis Infection.

    abstract::The fourth-generation QuantiFERON test for tuberculosis infection, QuantiFERON-TB Gold Plus (QFT-Plus) has replaced the earlier version, QuantiFERON-TB Gold In-Tube (QFT-GIT). A clinical need exists for information about agreement between QFT-Plus and other tests. We conducted this study to assess agreement of test re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00985-19

    authors: Venkatappa TK,Punnoose R,Katz DJ,Higgins MP,Banaei N,Graviss EA,Belknap RW,Ho CS

    更新日期:2019-10-23 00:00:00

  • Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.

    abstract::In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4486-4492.2002

    authors: Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

    更新日期:2002-12-01 00:00:00

  • Rapid identification of Staphylococcus aureus in blood cultures by use of the direct tube coagulase test.

    abstract::Direct tube coagulase testing for identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity, and positive and negative predicative values of 34%, 100%, 100%, and 80.2% with 2 h of incubation and 65%, 98.7, 99.7%, and 88.6% with 4 h of incubation. Anaerobic blood culture cont...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00369-07

    authors: Qian Q,Eichelberger K,Kirby JE

    更新日期:2007-07-01 00:00:00

  • Differentiation of Rhizomucor species by carbon source utilization and isoenzyme analysis.

    abstract::Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.7.2153-2156.1998

    authors: Vastag M,Papp T,Kasza Z,Vágvölgyi C

    更新日期:1998-07-01 00:00:00

  • Multicenter evaluation of the Vitek 2 anaerobe and Corynebacterium identification card.

    abstract::The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laborato...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00450-08

    authors: Rennie RP,Brosnikoff C,Turnbull L,Reller LB,Mirrett S,Janda W,Ristow K,Krilcich A

    更新日期:2008-08-01 00:00:00

  • Extended-spectrum-beta-lactamase production in a Salmonella enterica serotype Typhi strain from the Philippines.

    abstract::A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00676-08

    authors: Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

    更新日期:2008-08-01 00:00:00

  • Evaluation of the Sceptor microdilution antibiotic susceptibility testing system: a collaborative investigation.

    abstract::A multiwell, dried antimicrobial agent susceptibility test system, Sceptor (BBL Microbiology Systems, Cockeysville, Md.), was tested. The system was compared directly with a reference microdilution method by using two collections of stock cultures and 305 fresh clinical isolates. Sceptor was found to be in agreement (...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.1.184-194.1981

    authors: Jones RN,Thornsberry C,Barry AL,Gavan TL

    更新日期:1981-01-01 00:00:00

  • Choice of reference assay for the detection of rotavirus in fecal specimens: electron microscopy versus enzyme immunoassay.

    abstract::Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.6.1280-1283.1990

    authors: Dennehy PH,Gauntlett DR,Spangenberger SE

    更新日期:1990-06-01 00:00:00