Comparison of MGIT and Myco/F lytic liquid-based blood culture systems for recovery of Mycobacterium tuberculosis from pleural fluid.

abstract：:While foreign-born persons constitute only 11% of the population in the state of Rhode Island, they account for more than 65% of incident tuberculosis (TB) annually. We investigated the molecular-epidemiological differences between foreign-born and U.S.-born TB patients to estimate the degree of recent transmission an...

journal_title：Journal of clinical microbiology

authors： Vanhomwegen J,Kwara A,Martin M,Gillani FS,Fontanet A,Mutungi P,Crellin J,Obaro S,Gosciminski M,Carter EJ,Rastogi N

更新日期：2011-03-01 00:00:00

abstract：:The development of a rapid test to identify Mycobacterium tuberculosis Beijing isolates and specifically strain GC1237, coming from a sub-Saharan country, is needed due to its alarming wide spread on Gran Canaria Island (Spain). A rapid test that detects IS6110 present between dnaA and dnaN in the Beijing strains and ...

journal_title：Journal of clinical microbiology

authors： Millán-Lou MI,Alonso H,Gavín P,Hernández-Febles M,Campos-Herrero MI,Copado R,Cañas F,Kremer K,Caminero JA,Martín C,Samper S

更新日期：2012-02-01 00:00:00

abstract：:An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...

journal_title：Journal of clinical microbiology

authors： Penner JL,Hinton NA,Hennessy JN

更新日期：1976-04-01 00:00:00

abstract：:This study evaluated the performance of the Rapidec Carba NP test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing Enterobacteriaceae clinical isolates. In total, 252 clinical Enterobacteriaceae isolates that had been genetically ...

journal_title：Journal of clinical microbiology

authors： Hombach M,von Gunten B,Castelberg C,Bloemberg GV

更新日期：2015-12-01 00:00:00

abstract：:The development of a novel normalized quantitative competitive real-time PCR on the LightCycler instrument (NQC-LC-PCR) and its application to the quantification of cytomegalovirus (CMV) DNA in clinical samples are described. A heterologous competitor DNA was spiked into test samples and served as an internal amplific...

journal_title：Journal of clinical microbiology

authors： Stöcher M,Berg J

更新日期：2002-12-01 00:00:00

abstract：:A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

journal_title：Journal of clinical microbiology

authors： Holmberg O,Lyon RH,Price D,Farnsworth RJ

更新日期：1979-09-01 00:00:00

abstract：:The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A o...

journal_title：Journal of clinical microbiology

authors： Gibb TR,Norwood DA Jr,Woollen N,Henchal EA

更新日期：2001-11-01 00:00:00

abstract：:Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for a...

journal_title：Journal of clinical microbiology

authors： Vijayachari P,Ahmed N,Sugunan AP,Ghousunnissa S,Rao KR,Hasnain SE,Sehgal SC

更新日期：2004-08-01 00:00:00

abstract：:A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Polacheck I,Bennett JE

更新日期：1982-03-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...

journal_title：Journal of clinical microbiology

authors： von Riesen VL

更新日期：1975-12-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera ...

journal_title：Journal of clinical microbiology

authors： Heberling RL,Kalter SS,Smith JS,Hildebrand DG

更新日期：1987-07-01 00:00:00

abstract：:Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. W...

journal_title：Journal of clinical microbiology

authors： Lau R,Wang A,Chong-Kit A,Ralevski F,Boggild AK

更新日期：2015-04-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:The cobas human papillomavirus (HPV) test, approved by the FDA in April 2011, is a fully automated assay for the detection of 14 high-risk (hr) HPV genotypes from cervical specimens collected in liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes. Results are simultaneously ...

journal_title：Journal of clinical microbiology

authors： Rao A,Young S,Erlich H,Boyle S,Krevolin M,Sun R,Apple R,Behrens C

更新日期：2013-05-01 00:00:00

abstract：:The human pathogen Staphylococcus aureus is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presen...

journal_title：Journal of clinical microbiology

authors： Spence RP,Wright V,Ala-Aldeen DA,Turner DP,Wooldridge KG,James R

更新日期：2008-05-01 00:00:00

abstract：:PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus ...

journal_title：Journal of clinical microbiology

authors： Spilker T,Baldwin A,Bumford A,Dowson CG,Mahenthiralingam E,LiPuma JJ

更新日期：2009-08-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

journal_title：Journal of clinical microbiology

authors： Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

更新日期：2006-01-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:In an epidemiological study, nine isolates of dematiaceous fungi were recovered from the interior of a local feed and seed warehouse. Sample sites include brick walls and floors. Air samples also were included. Samples were collected in saline and plated on Mycobiotic and Sabouraud agar. The nine dematiaceous fungi re...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Kerkering TM,Shadomy HJ

更新日期：1982-04-01 00:00:00

abstract：:From 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. Eighty-three Veillonella spp. were recovered from 83 children (4%). Most Veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. The infections were polymicrobial in 79 (95%...

journal_title：Journal of clinical microbiology

authors： Brook I

更新日期：1996-05-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subje...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Iona E,Arzumanian E,Checchi F,Oggioni MR,Orefici G,Fattorini L

更新日期：2005-01-01 00:00:00

abstract：:The results of anaerobic susceptibility tests performed with inocula prepared directly from agar isolation media and from overnight broth cultures were compared. Altogether, 93.0% of the results with these two inoculum preparations were within one twofold dilution, including 92.5 and 95.6% of the results with Clostrid...

journal_title：Journal of clinical microbiology

authors： Murray PR,Niles AC

更新日期：1983-09-01 00:00:00

abstract：:We hypothesized that diversion of the first milliliter of venipuncture blood-the initial specimen diversion technique (ISDT)-would eliminate incompletely sterilized fragments of skin from the culture specimen and significantly reduce our blood culture contamination rate (R). We studied our hypothesis prospectively beg...

journal_title：Journal of clinical microbiology

authors： Patton RG,Schmitt T

更新日期：2010-12-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00