Invasive pneumococcal infection in a healthy infant caused by two different serotypes.

abstract：:Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80%...

journal_title：Journal of clinical microbiology

authors： Romero EC,Billerbeck AE,Lando VS,Camargo ED,Souza CC,Yasuda PH

更新日期：1998-05-01 00:00:00

abstract：:Tropheryma whipplei is the causative agent of classic Whipple's disease (WD) and other clinical entities, such as localized infection. Asymptomatic carriers have also been reported, mainly based on the testing of fecal samples. Our objective was to undertake a retrospective analysis of molecular biology usage for the ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Fenollar F,Raoult D

更新日期：2012-12-01 00:00:00

abstract：:Bacteroides forsythus has been associated with destructive adult periodontitis. Up to now, detailed analysis by classical means was hampered by the fastidious nature of the organism. There is hope that the application of molecular detection methods such as indirect immunofluorescence or in situ hybridization (ISH) wil...

journal_title：Journal of clinical microbiology

authors： Gersdorf H,Meissner A,Pelz K,Krekeler G,Göbel UB

更新日期：1993-04-01 00:00:00

abstract：:This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

更新日期：1991-01-01 00:00:00

abstract：:A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire...

journal_title：Journal of clinical microbiology

authors： Chen JHK,Lam HY,Yip CCY,Wong SCY,Chan JFW,Ma ESK,Cheng VCC,Tang BSF,Yuen KY

更新日期：2016-07-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum ...

journal_title：Journal of clinical microbiology

authors： Shekarchi IC,Sever JL,Tzan N,Ley A,Ward LC,Madden D

更新日期：1981-05-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:In a group of 596 delivering Ghanaian women, the sensitivities of peripheral blood thick film microscopy, ICT Malaria P.f/P.v test, and PCR in detecting microscopically confirmed placental Plasmodium falciparum infection were 42, 80, and 97%, respectively. In addition to the gross underestimation of placental malaria ...

journal_title：Journal of clinical microbiology

authors： Mockenhaupt FP,Ulmen U,von Gaertner C,Bedu-Addo G,Bienzle U

更新日期：2002-01-01 00:00:00

abstract：:Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had s...

journal_title：Journal of clinical microbiology

authors： Norder H,Karlsson M,Mellgren Å,Konar J,Sandberg E,Lasson A,Castedal M,Magnius L,Lagging M

更新日期：2016-03-01 00:00:00

abstract：:A patient with a dual Salmonella infection is described. Salmonella group B was recovered from three blood culture sets but was not detected in seven stool cultures. Salmonella group C2 was isolated from three of seven stool cultures but was not recovered from blood cultures. Specific, non-cross-reactive antibodies to...

journal_title：Journal of clinical microbiology

authors： Papasian CJ,Bartholomew WR,Neter E,Amsterdam D

更新日期：1984-09-01 00:00:00

abstract：:Severe emaciation and mortalities suggestive of mycobacterial infections were recently reported for both adult and young wild red deer (Cervus elaphus) in the southeastern part of Belgium. In deer, tuberculous lesions are not pathognomonic of Mycobacterium bovis infection due to gross and microscopic similarities with...

journal_title：Journal of clinical microbiology

authors： Godfroid J,Delcorps C,Irenge LM,Walravens K,Marché S,Gala JL

更新日期：2005-09-01 00:00:00

abstract：:We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In ...

journal_title：Journal of clinical microbiology

authors： Grange PA,Jary A,Isnard C,Burrel S,Boutolleau D,Touati A,Bébéar C,Saule J,Martinet P,Robert JL,Moulene D,Vermersch-Langlin A,Benhaddou N,Janier M,Dupin N

更新日期：2021-01-21 00:00:00

abstract：:The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resu...

journal_title：Journal of clinical microbiology

authors： Vatanshenassan M,Boekhout T,Lass-Flörl C,Lackner M,Schubert S,Kostrzewa M,Sparbier K

更新日期：2018-08-27 00:00:00

abstract：:A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

journal_title：Journal of clinical microbiology

authors： Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

更新日期：2011-11-01 00:00:00

abstract：:We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and ...

journal_title：Journal of clinical microbiology

authors： Godreuil S,Torrea G,Terru D,Chevenet F,Diagbouga S,Supply P,Van de Perre P,Carriere C,Bañuls AL

更新日期：2007-03-01 00:00:00

abstract：:We have developed a rapid and sensitive assay for the detection of Salmonella serovars in veterinary clinical specimens. This method utilizes a short cultivation period followed by PCR. For detection of the amplified product, an enzyme-linked immunosorbent assay (ELISA)-based oligonucleotide ligation assay (OLA) was u...

journal_title：Journal of clinical microbiology

authors： Stone GG,Oberst RD,Hays MP,McVey S,Chengappa MM

更新日期：1995-11-01 00:00:00

abstract：:This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroi...

journal_title：Journal of clinical microbiology

authors： Hien BT,Scheutz F,Cam PD,Serichantalergs O,Huong TT,Thu TM,Dalsgaard A

更新日期：2008-03-01 00:00:00

abstract：:Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and speci...

journal_title：Journal of clinical microbiology

authors： Kelley PG,Grabsch EA,Howden BP,Gao W,Grayson ML

更新日期：2009-11-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-...

journal_title：Journal of clinical microbiology

authors： Bonnet R,Souweine B,Gauthier G,Rich C,Livrelli V,Sirot J,Joly B,Forestier C

更新日期：1998-06-01 00:00:00

abstract：:The absence of markers of inflammation in the cerebrospinal fluid (CSF) commonly predicts the absence of herpes simplex virus (HSV) central nervous system (CNS) infection. Consequently, multiple authors have proposed and validated criteria for deferring HSV PCR testing of CSF in immunocompetent hosts with normal CSF w...

journal_title：Journal of clinical microbiology

authors： Hauser RG,Campbell SM,Brandt CA,Wang S

更新日期：2017-05-01 00:00:00

abstract：:We compared the results obtained with six commercial MIC test systems (Etest, MicroScan, Phoenix, Sensititre, Vitek Legacy, and Vitek 2 systems) and three reference methods (agar dilution, disk diffusion, and vancomycin [VA] agar screen [VScr]) with the results obtained by the Clinical and Laboratory Standards Institu...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Anderson KF,Lonsway DR,Thompson A,McAllister SK,Limbago BM,Carey RB,Tenover FC,Patel JB

更新日期：2009-07-01 00:00:00

abstract：:Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

更新日期：2007-06-01 00:00:00

abstract：:Thirty acquired immunodeficiency syndrome patients with mycobacterial bacteremia documented by Du Pont Isolator (Du Pont Co., Wilmington, Del.) blood cultures underwent microscopic examination of buffy coat blood smears. Of 30 patients, 14 were culture positive for Mycobacterium avium-Mycobacterium intracellulare comp...

journal_title：Journal of clinical microbiology

authors： Eng RH,Bishburg E,Smith SM,Mangia A

更新日期：1989-04-01 00:00:00

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

更新日期：2009-10-01 00:00:00

abstract：:Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...

journal_title：Journal of clinical microbiology

authors： Kumar A,Shukla D,Kumar R,Idris MZ,Misra UK,Dhole TN

更新日期：2012-11-01 00:00:00