Pneumococcus with the "6E" cps Locus Produces Serotype 6B Capsular Polysaccharide.

Abstract:

:Genetic studies of serogroup 6 isolates ofStreptococcus pneumoniaeidentified putative serotype 6E. Although its capsular polysaccharide structure has not been elucidated, putative serotype 6E is described in an increasing number of studies as a potentially new serotype. We show here that SPEC6B, which is widely used as a target strain for serotype 6B opsonophagocytosis assays, has the genetic features of the putative serotype 6E but produces capsular polysaccharide identical to 6B capsular polysaccharide as determined by one-dimensional (1D) and 2D nuclear magnetic resonance (NMR). Thus, putative serotype 6E is a mere genetic variant of serotype 6B. Also, SPEC6B is appropriate as a target strain for serotype 6B opsonophagocytosis assays. This example illustrates the difficulties of assigning new bacterial serotypes based on genetic findings alone.

journal_name

J Clin Microbiol

authors

Burton RL,Geno KA,Saad JS,Nahm MH

doi

10.1128/JCM.03194-15

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

967-71

issue

4

eissn

0095-1137

issn

1098-660X

pii

JCM.03194-15

journal_volume

54

pub_type

杂志文章
  • Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives.

    abstract::A rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. Using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of Porphyromonas gingivalis, Prevotella intermedia,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.9.1955-1958.1991

    authors: Moncla BJ,Braham P,Rabe LK,Hillier SL

    更新日期:1991-09-01 00:00:00

  • Native valve endocarditis caused by an organism resembling Corynebacterium striatum.

    abstract::An organism resembling Corynebacterium striatum was isolated from the blood of a patient with acute aortic valvular insufficiency and no history of valvular heart disease. At autopsy, histopathologic examination of the aortic valve revealed pleomorphic gram-positive bacilli and destruction of valvular tissue. Our isol...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.1.8-10.1990

    authors: Markowitz SM,Coudron PE

    更新日期:1990-01-01 00:00:00

  • Molecular characterization of two Bordetella bronchiseptica strains isolated from children with coughs.

    abstract::During a surveillance program associated with the Italian clinical trial for the evaluation of new acellular pertussis vaccines, two bacterial isolates were obtained in cultures of samples from immunocompetent infants who had episodes of cough. Both clinical isolates were identified as Bordetella bronchiseptica by bio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.6.1550-1555.1997

    authors: Stefanelli P,Mastrantonio P,Hausman SZ,Giuliano M,Burns DL

    更新日期:1997-06-01 00:00:00

  • Characterization of two DNA probes specific for Serpulina hyodysenteriae.

    abstract::Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.7.1746-1752.1993

    authors: Sotiropoulos C,Smith SC,Coloe PJ

    更新日期:1993-07-01 00:00:00

  • Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

    abstract::A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.8.4178-4182.2005

    authors: Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

    更新日期:2005-08-01 00:00:00

  • Diagnostic implications of parasite-specific immune responses in immunocompromised patients with strongyloidiasis.

    abstract::Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.6.1099-1103.1986

    authors: Genta RM,Douce RW,Walzer PD

    更新日期:1986-06-01 00:00:00

  • Gemella morbillorum bacteremia after anti-tumor necrosis factor alpha as acne inversa therapy.

    abstract::We present a case of fever, brain abscesses, and Gemella morbillorum bacteremia after anti-tumor necrosis factor alpha (TNF-α) therapy in a 21-year-old acne inversa patient currently taking long-term dapsone. To the best of our knowledge, this is the first report describing such a case. During antimicrobial therapy, t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06161-11

    authors: Vossen MG,Gattringer KB,Khalifeh N,Koreny M,Spertini V,Mallouhi A,Willeit M,Volc-Platzer B,Asboth F,Graninger W,Thalhammer F,Lagler H

    更新日期:2012-03-01 00:00:00

  • Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists' Mycobacteriology E survey: results of a 1993 questionnaire.

    abstract::Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.2.407-412.1995

    authors: Woods GL,Witebsky FG

    更新日期:1995-02-01 00:00:00

  • Mycoplasma hominis-associated parapharyngeal abscess following acute Epstein-Barr virus infection in a previously immunocompetent adult.

    abstract::Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal ab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02203-08

    authors: Kennedy KJ,Prince S,Makeham T

    更新日期:2009-09-01 00:00:00

  • Variable assimilation of carbon compounds by Candida albicans.

    abstract::A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.1.163-166.1981

    authors: Syverson RE

    更新日期:1981-01-01 00:00:00

  • Blinded comparison of a direct immunofluorescent monoclonal antibody staining method and a Giemsa staining method for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens of patients infected with human immunodefic

    abstract::A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.9.2136-2138.1990

    authors: Wolfson JS,Waldron MA,Sierra LS

    更新日期:1990-09-01 00:00:00

  • Survival of aerobic and anaerobic bacteria in purulent clinical specimens maintained in the Copan Venturi Transystem and Becton Dickinson Port-a-Cul transport systems.

    abstract::Recovery of aerobic and anaerobic bacteria from clinical specimens maintained in the Copan Venturi Transystem and the Becton Dickinson Port-a-Cul transport was assessed. Of 54 anaerobes, 53 were recovered after 4 h, and 52 were recovered after 24 h, from both systems. After 48 h, 45 and 50 were recovered from the two ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.2.892-894.2000

    authors: Citron DM,Warren YA,Hudspeth MK,Goldstein EJ

    更新日期:2000-02-01 00:00:00

  • Comparison of the Chinese schema and the International Antigenic Typing System for serotyping Pseudomonas aeruginosa.

    abstract::Twelve strains of Pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the People's Republic of China were compared with serogroups in the International Antigenic Typing System (IATS). The first eight groups originated in the People's Republic of China, and group II appears to have a ne...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.5.824-826.1987

    authors: Liu PV

    更新日期:1987-05-01 00:00:00

  • Antibody to a 39-kilodalton Borrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals.

    abstract::Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.2.236-243.1991

    authors: Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

    更新日期:1991-02-01 00:00:00

  • Comparing QuantiFERON-TB Gold Plus with Other Tests To Diagnose Mycobacterium tuberculosis Infection.

    abstract::The fourth-generation QuantiFERON test for tuberculosis infection, QuantiFERON-TB Gold Plus (QFT-Plus) has replaced the earlier version, QuantiFERON-TB Gold In-Tube (QFT-GIT). A clinical need exists for information about agreement between QFT-Plus and other tests. We conducted this study to assess agreement of test re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00985-19

    authors: Venkatappa TK,Punnoose R,Katz DJ,Higgins MP,Banaei N,Graviss EA,Belknap RW,Ho CS

    更新日期:2019-10-23 00:00:00

  • Gene mutations responsible for overexpression of AmpC beta-lactamase in some clinical isolates of Enterobacter cloacae.

    abstract::AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutatio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.6.2955-2958.2005

    authors: Kaneko K,Okamoto R,Nakano R,Kawakami S,Inoue M

    更新日期:2005-06-01 00:00:00

  • Prophage sequences defining hot spots of genome variation in Salmonella enterica serovar Typhimurium can be used to discriminate between field isolates.

    abstract::Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00729-07

    authors: Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

    更新日期:2007-08-01 00:00:00

  • Antimicrobial-Loaded Bone Cement Does Not Negatively Influence Sonicate Fluid Culture Positivity for Diagnosis of Prosthetic Joint Infection.

    abstract::We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cem...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00516-16

    authors: Park KH,Greenwood-Quaintance KE,Hanssen AD,Abdel MP,Patel R

    更新日期:2016-06-01 00:00:00

  • Bacteriology of dental abscesses of endodontic origin.

    abstract::Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.4.770-774.1983

    authors: Williams BL,McCann GF,Schoenknecht FD

    更新日期:1983-10-01 00:00:00

  • Identification and molecular characterization of a bovine G3 rotavirus which causes age-independent diarrhea in cattle.

    abstract::G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.3.937-942.2002

    authors: El-Attar L,Dhaliwal W,Iturriza-Gómara M,Bridger JC

    更新日期:2002-03-01 00:00:00

  • Performance of human immunodeficiency virus type 1 gp41 assays for detecting enfuvirtide (T-20) resistance mutations.

    abstract::The performance of a gp41 assay, created using reagents designed for use with the OpenGene DNA Sequencing System, was evaluated using a panel of plasma samples obtained from enfuvirtide-naive and -experienced human immunodeficiency virus type 1-infected subjects. Resulting sequence data were highly accurate compared t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00666-06

    authors: Marcial M,Lu J,Deeks SG,Ziermann R,Kuritzkes DR

    更新日期:2006-09-01 00:00:00

  • Comparison of methods for serotyping isolates of Haemophilus influenzae.

    abstract::This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.2.158-160.1985

    authors: Himmelreich CA,Barenkamp SJ,Storch GA

    更新日期:1985-02-01 00:00:00

  • Epidemiology of enterovirus 71 in the Netherlands, 1963 to 2008.

    abstract::The incidence of enterovirus 71 (EV71) infection has greatly increased in the Asian Pacific region since 1997. Several large outbreaks, caused by different subgenogroups of EV71, occurred with high rates of morbidity and a substantial number of deaths. In 2007, 58 cases of EV71 infection requiring hospitalization were...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00507-09

    authors: van der Sanden S,Koopmans M,Uslu G,van der Avoort H,Dutch Working Group for Clinical Virology.

    更新日期:2009-09-01 00:00:00

  • Prolonged Bartonella bacteremia in cats associated with cat-scratch disease patients.

    abstract::Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.12.3245-3251.1995

    authors: Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

    更新日期:1995-12-01 00:00:00

  • Comparison of three methods for rapid identification of mycobacterial clinical isolates to the species level.

    abstract::A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02253-06

    authors: Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

    更新日期:2007-06-01 00:00:00

  • Antigenic relationships among the porin proteins of encapsulated Haemophilus influenzae clones.

    abstract::Monoclonal antibodies (Mabs) specific for Haemophilus influenzae were generated to identify antigenic determinants shared among encapsulated H. influenzae clones. Sixteen MAbs reacted by Western immunoblot with a protein of an approximate molecular size of 40 kilodaltons corresponding to the P2 major outer membrane pr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.8.1720-1724.1990

    authors: Martin D,Hamel J,Brodeur BR,Musser JM

    更新日期:1990-08-01 00:00:00

  • Genetic heterogeneity among strains of Treponema phagedenis-like spirochetes isolated from dairy cattle with papillomatous digital dermatitis in Japan.

    abstract::Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01574-08

    authors: Yano T,Yamagami R,Misumi K,Kubota C,Moe KK,Hayashi T,Yoshitani K,Ohtake O,Misawa N

    更新日期:2009-03-01 00:00:00

  • Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8.

    abstract::Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simula...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.2.358-360.1990

    authors: Poutrel B,Mendolia C,Sutra L,Fournier JM

    更新日期:1990-02-01 00:00:00

  • Frequency of Positive Aspergillus Tests in COVID-19 Patients in Comparison to Other Patients with Pulmonary Infections Admitted to the ICU.

    abstract::The aim of this study was to describe the frequency of positive Aspergillus tests in COVID-19 patients and investigate the association between COVID-19 and a positive Aspergillus test result. We compared the proportion of positive Aspergillus tests in COVID-19 patients admitted to the ICU for > 24 hours with two contr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02278-20

    authors: Yusuf E,Vonk A,van den Akker JPC,Bode L,Sips GJ,Rijnders BJA,de Steenwinkel J,Verkaik NJ,Vogel M,van der Eerden M,van Westreenen M

    更新日期:2020-12-04 00:00:00

  • Comparison of PCR and culture to the indirect fluorescent-antibody test for diagnosis of Potomac horse fever.

    abstract::Potomac horse fever is an acute systemic equine disease caused by Ehrlichia risticii. Currently, serologic methods are widely used to diagnose this disease. However, serologic methods cannot determine whether the horse is presently infected or has been exposed to ehrlichial antigens in the past. The purpose of the pre...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.9.2215-2219.1997

    authors: Mott J,Rikihisa Y,Zhang Y,Reed SM,Yu CY

    更新日期:1997-09-01 00:00:00