Abstract:
:Genetic studies of serogroup 6 isolates ofStreptococcus pneumoniaeidentified putative serotype 6E. Although its capsular polysaccharide structure has not been elucidated, putative serotype 6E is described in an increasing number of studies as a potentially new serotype. We show here that SPEC6B, which is widely used as a target strain for serotype 6B opsonophagocytosis assays, has the genetic features of the putative serotype 6E but produces capsular polysaccharide identical to 6B capsular polysaccharide as determined by one-dimensional (1D) and 2D nuclear magnetic resonance (NMR). Thus, putative serotype 6E is a mere genetic variant of serotype 6B. Also, SPEC6B is appropriate as a target strain for serotype 6B opsonophagocytosis assays. This example illustrates the difficulties of assigning new bacterial serotypes based on genetic findings alone.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Burton RL,Geno KA,Saad JS,Nahm MHdoi
10.1128/JCM.03194-15subject
Has Abstractpub_date
2016-04-01 00:00:00pages
967-71issue
4eissn
0095-1137issn
1098-660Xpii
JCM.03194-15journal_volume
54pub_type
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