Comparison of methods for serotyping isolates of Haemophilus influenzae.

abstract：:psaA is the gene encoding pneumococcal surface adhesin A (PsaA), a 37-kDa protein expressed on the surface of Streptococcus pneumoniae. PCR primers for psaA have been shown to amplify the target DNA sequence in all 90 serotypes of S. pneumoniae and in none of 67 heterologous pathogens and colonizing bacteria of the up...

journal_title：Journal of clinical microbiology

authors： Scott JA,Marston EL,Hall AJ,Marsh K

更新日期：2003-06-01 00:00:00

abstract：:An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglu...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Thulliez P,Desmonts G,Remington JS

更新日期：1988-05-01 00:00:00

abstract：:Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC devel...

journal_title：Journal of clinical microbiology

authors： Williams MM,Taylor TH Jr,Warshauer DM,Martin MD,Valley AM,Tondella ML

更新日期：2015-01-01 00:00:00

abstract：:We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshol...

journal_title：Journal of clinical microbiology

authors： Bennett A,Bar-Zeev N,Jere KC,Tate JE,Parashar UD,Nakagomi O,Heyderman RS,French N,Iturriza-Gomara M,Cunliffe NA

更新日期：2015-06-01 00:00:00

abstract：:Brucellosis in Argentina is currently investigated in bank donor blood by the standard plate agglutination test (PAT). This study evaluated the buffered plate antigen test (BPA), now used to screen for bovine brucellosis, as a screen for human disease. Of 57 sera from patients with culture-confirmed brucellosis, 100% ...

journal_title：Journal of clinical microbiology

authors： Lucero NE,Bolpe JE

更新日期：1998-05-01 00:00:00

abstract：:We describe the first case of Epstein-Barr virus (EBV)-associated thymic carcinoid tumor found by in situ hybridization (ISH) on paraffin-embedded sections. ISH revealed that both tumor cells and infiltrated lymphocytes were EBV positive, while a few EBV-infected lymphocytes were detected in 2 of 11 thymuses and 1 of ...

journal_title：Journal of clinical microbiology

authors： Takeuchi H,Fujita H,Iwasaki F,Takeuchi T,Imadome K,Okumiya T,Matsui T,Takahashi M

更新日期：2004-06-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:Candida albicans is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating C. albicans from other Candida species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, ...

journal_title：Journal of clinical microbiology

authors： Kim HJ,Brehm-Stecher BF

更新日期：2015-02-01 00:00:00

abstract：:Hybrid Shiga toxin-producing Escherichia coli (STEC) and uropathogenic E. coli (UPEC) strains of multilocus sequence type 141 (ST141) cause both urinary tract infections and diarrhea in humans and are phylogenetically positioned between STEC and UPEC strains. We used comparative genomic analysis of 85 temporally and s...

journal_title：Journal of clinical microbiology

authors： Gati NS,Middendorf-Bauchart B,Bletz S,Dobrindt U,Mellmann A

更新日期：2019-12-23 00:00:00

abstract：:In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

journal_title：Journal of clinical microbiology

authors： Griffiths TA,Rioux K,De Buck J

更新日期：2008-04-01 00:00:00

abstract：:We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar and Mueller-Hinton ag...

journal_title：Journal of clinical microbiology

authors： Lally RT,Ederer MN,Woolfrey BF

更新日期：1985-10-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is now routinely performed in clinical microbiology laboratories to assess isolate relatedness. With appropriately developed analytics, the same data can be used for prediction of antimicrobial susceptibility. We assessed WGS data for identification using open-source tools and antibiotic ...

journal_title：Journal of clinical microbiology

authors： Ferreira I,Beisken S,Lueftinger L,Weinmaier T,Klein M,Bacher J,Patel R,von Haeseler A,Posch AE

更新日期：2020-06-24 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, S...

journal_title：Journal of clinical microbiology

authors： Pingle MR,Granger K,Feinberg P,Shatsky R,Sterling B,Rundell M,Spitzer E,Larone D,Golightly L,Barany F

更新日期：2007-06-01 00:00:00

abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

journal_title：Journal of clinical microbiology

authors： Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

更新日期：2002-02-01 00:00:00

abstract：:From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequ...

journal_title：Journal of clinical microbiology

authors： Tokatlidou D,Tsivitanidou M,Pournaras S,Ikonomidis A,Tsakris A,Sofianou D

更新日期：2008-03-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

journal_title：Journal of clinical microbiology

authors： Marne C,Pallarés R,Sitges-Serra A

更新日期：1983-06-01 00:00:00

abstract：:Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans wa...

journal_title：Journal of clinical microbiology

authors： Jahn B,Stüben A,Bhakdi S

更新日期：1996-08-01 00:00:00

abstract：:Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid ex...

journal_title：Journal of clinical microbiology

authors： Hafez K,El Kholy AM,Facklam RR

更新日期：1981-11-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population. Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of symptoms. ...

journal_title：Journal of clinical microbiology

authors： Bitsko RH,Cortese MM,Dayan GH,Rota PA,Lowe L,Iversen SC,Bellini WJ

更新日期：2008-03-01 00:00:00

abstract：:Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these st...

journal_title：Journal of clinical microbiology

authors： Retter M,Middleton PJ,Tam JS,Petric M

更新日期：1979-10-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

更新日期：2004-12-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:Diagnostic laboratories are under increasing pressure to improve and expand their services. Greater flexibility in sample processing is a critical factor that can improve the time to results while reducing reagent waste, making laboratories more efficient and cost-effective. The introduction of the Abbott mPLUS featur...

journal_title：Journal of clinical microbiology

authors： Lucic D,Jones S,Wiesneth R,Barry C,Webb E,Belova L,Dolan P,Ho S,Abravaya K,Cloherty G

更新日期：2013-12-01 00:00:00

abstract：:Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi o...

journal_title：Journal of clinical microbiology

authors： Liveris D,Wang G,Girao G,Byrne DW,Nowakowski J,McKenna D,Nadelman R,Wormser GP,Schwartz I

更新日期：2002-04-01 00:00:00

abstract：:The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

journal_title：Journal of clinical microbiology

authors： Smith RA,DiGiorgio S,Darner J,Wilhelm A

更新日期：1981-04-01 00:00:00

abstract：:Specimens from skin lesions were examined simultaneously for herpes simplex virus (HSV) and varicella-zoster virus (VZV) by direct specimen testing and shell vial culture in single-test systems. For direct testing, cells in a single specimen well were stained with a combination direct-indirect immunofluorescence stain...

journal_title：Journal of clinical microbiology

authors： Brumback BG,Farthing PG,Castellino SN

更新日期：1993-12-01 00:00:00