Abstract:
:Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay was developed for the detection of B. anthracis. After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Hurtle W,Bode E,Kulesh DA,Kaplan RS,Garrison J,Bridge D,House M,Frye MS,Loveless B,Norwood Ddoi
10.1128/jcm.42.1.179-185.2004subject
Has Abstractpub_date
2004-01-01 00:00:00pages
179-85issue
1eissn
0095-1137issn
1098-660Xjournal_volume
42pub_type
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