Simultaneous detection of influenza viruses A and B using real-time quantitative PCR.

abstract：:Herpes simplex virus (HSV) causes acute and relapsing symptoms characterized by ulcerative lesions. Laboratory diagnosis of HSV in cutaneous or mucocutaneous lesions has historically been performed with the use of viral cell culture systems; however, these tests are laborious and suffer decreased sensitivity for advan...

journal_title：Journal of clinical microbiology

authors： Faron ML,Ledeboer NA,Patel A,Beqa SH,Yen-Lieberman B,Kohn D,Leber AL,Mayne D,Northern WI,Buchan BW

更新日期：2016-08-01 00:00:00

abstract：:Restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic Escherichia coli. To compare their relative efficiencies, these two sets of probes were radiolabel...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Kalland KH,Raj P,Moseley SL,Bhan MK,Bjorvatn B

更新日期：1988-11-01 00:00:00

abstract：:The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...

journal_title：Journal of clinical microbiology

authors： Clarke SC,Diggle MA,Edwards GF

更新日期：2001-09-01 00:00:00

abstract：:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses. ...

journal_title：Journal of clinical microbiology

authors： Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli M

更新日期：2001-05-01 00:00:00

abstract：:Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good a...

journal_title：Journal of clinical microbiology

authors： Jones RN,Wilson HW,Novick WJ Jr

更新日期：1982-04-01 00:00:00

abstract：:A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis...

journal_title：Journal of clinical microbiology

authors： Robertson EA,MacLowry JD

更新日期：1975-06-01 00:00:00

abstract：:We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Grewal HM,Gaastra W,Svennerholm AM,Bhan MK

更新日期：1992-07-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:An 840-bp fragment of the 18S rRNA gene was used to identify Cryptosporidium spp. recovered from human immunodeficiency virus (HIV)-infected and -uninfected patients from Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. Initial identification was by Ziehl-Neelsen acid-fast staining. Confirmation was by nested P...

journal_title：Journal of clinical microbiology

authors： Gatei W,Greensill J,Ashford RW,Cuevas LE,Parry CM,Cunliffe NA,Beeching NJ,Hart CA

更新日期：2003-04-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for detection of immunoglobulin M (IgM) and IgG antibodies to Mycoplasma pneumoniae was developed. The EIA was evaluated on the basis of results in the M. pneumoniae complement fixation (MPCF) test and the cold agglutinin test. Serum samples from 430 patients with respiratory infections of ...

journal_title：Journal of clinical microbiology

authors： Uldum SA,Jensen JS,Søndergård-Andersen J,Lind K

更新日期：1992-05-01 00:00:00

abstract：:A cluster of Mycobacterium ulcerans infections has recently occurred on Phillip Island, Victoria, Australia. Previous cases of infection have generally been located around Bairnsdale in southeast Gippsland. The aim of this study was to determine the epidemiological relationship between these strains and other strains ...

journal_title：Journal of clinical microbiology

authors： Jackson K,Edwards R,Leslie DE,Hayman J

更新日期：1995-09-01 00:00:00

abstract：:Because of their biofilm-forming capacity, invasive Staphylococcus epidermidis isolates, which cause the majority of nosocomial catheter-related bloodstream infections (BSIs), are thought to be selected at the time of catheter insertion from a population of less virulent commensal strains. This fact allows the predict...

journal_title：Journal of clinical microbiology

authors： Rohde H,Kalitzky M,Kröger N,Scherpe S,Horstkotte MA,Knobloch JK,Zander AR,Mack D

更新日期：2004-12-01 00:00:00

abstract：:We describe the first fatal evolution of cellulitis due to Neisseria meningitidis serogroup Y involving an 85-year-old woman. She presented with an extensive cellulitis of the left side of the face, neck, and thorax and septic shock. In spite of active antibiotic therapy, evolution was rapidly fatal. ...

journal_title：Journal of clinical microbiology

authors： Cartolano GL,Barbier C,Arnoult L,Simon D,Ricome JL,Hayon J

更新日期：2003-08-01 00:00:00

abstract：:This study examines the microbiological and epidemiological characteristics of toxigenic and non-toxigenic Corynebacterium isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Ep...

journal_title：Journal of clinical microbiology

authors： Hoefer A,Pampaka D,Herrera-León S,Peiró S,Varona S,López-Perea N,Masa-Calles J,Herrera-León L

更新日期：2020-12-09 00:00:00

abstract：:We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strain...

journal_title：Journal of clinical microbiology

authors： Paton AW,Paton JC

更新日期：2002-01-01 00:00:00

abstract：:The taxonomic relationship of Rhinosporidium seeberi with other organisms remained controversial for over a century. Recently, molecular studies have shown R. seeberi to be a protistal microbe in the newly described class Mesomycetozoea at the animal-fungal boundary. Phylogenetic analyses of R. seeberi using 18S small...

journal_title：Journal of clinical microbiology

authors： Silva V,Pereira CN,Ajello L,Mendoza L

更新日期：2005-04-01 00:00:00

abstract：:We describe two cases of Clostridium glycolicum wound infections in immunocompetent adults. The bacterium was identified by 16S rRNA gene sequencing. This is the third published report of the recovery of this organism from human clinical material and highlights the importance of the organism as a potential human patho...

journal_title：Journal of clinical microbiology

authors： Jiang W,Abrar S,Romagnoli M,Carroll KC

更新日期：2009-05-01 00:00:00

abstract：:Anaerobes are an important but uncommon cause of bloodstream infections (BSIs). For pediatric patients, routine inclusion of an anaerobic blood culture alongside the aerobic remains controversial. We implemented automatic anaerobic blood culture alongside aerobic blood cultures in a pediatric emergency department (ED)...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Chang TP,Yee R,Manshadi K,Lichtenfeld N,Choi HJ,Festekjian A

更新日期：2020-08-24 00:00:00

abstract：:Duck hepatitis B virus (DHBV) belongs to the Hepadnaviridae family, which includes human Hepatitis B virus (HBV) and Woodchuck hepatitis virus. It is widely distributed in wild and domestic ducks due to congenital transmission. HBV is a worldwide health problem, with carriers at risk of developing cirrhosis and liver ...

journal_title：Journal of clinical microbiology

authors： Wang CY,Giambrone JJ,Smith BF

更新日期：2002-07-01 00:00:00

abstract：:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...

journal_title：Journal of clinical microbiology

authors： Pietilä J,He Q,Oksi J,Viljanen MK

更新日期：2000-07-01 00:00:00

abstract：:Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

更新日期：1995-01-01 00:00:00

abstract：:The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

journal_title：Journal of clinical microbiology

authors： Kloos WE,Wolfshohl JF

更新日期：1982-09-01 00:00:00

abstract：:Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-de...

journal_title：Journal of clinical microbiology

authors： Helb D,Jones M,Story E,Boehme C,Wallace E,Ho K,Kop J,Owens MR,Rodgers R,Banada P,Safi H,Blakemore R,Lan NT,Jones-López EC,Levi M,Burday M,Ayakaka I,Mugerwa RD,McMillan B,Winn-Deen E,Christel L,Dailey P,Perkins

更新日期：2010-01-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

journal_title：Journal of clinical microbiology

authors： Fridriksdóttir V,Nesse LL,Gudding R

更新日期：1992-05-01 00:00:00

abstract：:Septic arthritis of the knee developed in a 21-month-old child. The causative organism, isolated from two separate arthrocenteses, was identified as Capnocytophaga ochracea morphologically and by biochemical reactions. Previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant ora...

journal_title：Journal of clinical microbiology

authors： Winn RE,Chase WF,Lauderdale PW,McCleskey FK

更新日期：1984-04-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:A total of 201 Mycoplasma pneumoniae clinical isolates from Beijing, China, isolated from 2008 to 2011, were clustered into 16 multiple-locus variable-number tandem-repeat analysis (MLVA) types, of which 6 new MLVA types have never been reported previously. Type 1 isolates based on p1 gene genotyping were mainly MLVA ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Liu G,Cao B,Wu J,Gu Y,He L,Meng F,Zhu L,Yin Y,Lv M,Zhang J

更新日期：2013-02-01 00:00:00

abstract：:Lactococcus garvieae is a Gram-positive coccus that has morphological and biochemical similarities to enterococci. L. garvieae strains rare human pathogens, with only a few cases reported in the literature, mainly as a cause of infective endocarditis. L. garvieae is well known as a fish pathogen, and in some of the re...

journal_title：Journal of clinical microbiology

authors： Navas ME,Hall G,El Bejjani D

更新日期：2013-06-01 00:00:00