Abstract:
:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplified product. A total of 32 DNA samples were tested. Of them three were isolated from B. burgdorferi reference strains and 16 were isolated from B. burgdorferi strains cultured from Ixodes ricinus ticks; 13 were directly isolated from nine human biopsy specimens and four I. ricinus tick midguts. The melting temperature of B. garinii was 2 degrees C lower than that of B. burgdorferi sensu stricto and B. afzelii. Melting curve analysis offers a rapid alternative for identification and detection of B. burgdorferi sensu lato genospecies.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Pietilä J,He Q,Oksi J,Viljanen MKdoi
10.1128/JCM.38.7.2756-2759.2000subject
Has Abstractpub_date
2000-07-01 00:00:00pages
2756-9issue
7eissn
0095-1137issn
1098-660Xjournal_volume
38pub_type
杂志文章abstract::First-catch early morning urine samples from patients attending a genitourinary medicine clinic were tested by three different enzyme immunoassays. The results suggest significant differences in the sensitivities and specificities of the different assays. The direct visualization of elementary bodies in urine deposits...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.2.220-222.1990
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
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abstract::We compared serial measurements of antibodies to mannan and to a cytoplasmic antigen (enzyme-linked immunosorbent assays), detection of mannan and an unidentified candidal antigen (latex agglutination), and assays of mannose and arabinitol (gas chromatographic assay of per-O-acetylated aldonitrile derivatives). In a h...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.3.409-417.1988
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abstract::Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...
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doi:10.1128/JCM.35.7.1715-1721.1997
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.3.814-817.1998
更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JCM.01028-14
更新日期:2014-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.01633-06
更新日期:2007-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01687-08
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pub_type: 杂志文章
doi:10.1128/jcm.42.4.1753-1755.2004
更新日期:2004-04-01 00:00:00
abstract::Polyomavirus BK (BKV) causes asymptomatic latent infection in the human host that is reactivated during periods of immune suppression. Detection by conventional tube cell culture is difficult and time consuming because BKV exhibits slow growth with late (14 to 28 days) and subtle cytopathic effects. We developed a she...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.7.1613-1615.1990
更新日期:1990-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.4.887-894.1993
更新日期:1993-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.11.4072-4075.2000
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.31.8.2057-2060.1993
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.19.3.338-341.1984
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.30.1.176-180.1992
更新日期:1992-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2009-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
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abstract::The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoas...
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journal_title:Journal of clinical microbiology
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更新日期:2008-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene (A. Telenti, F. Marchesi, M. Balz, F. Bally, E. C. Böttger, and T. Bodmer, J. Clin. Microbiol. 31:175-178, 1993) was applied to 108 mycobacterial isolates representing 34 species to evaluate its potential as a rapid reference method. A total...
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pub_type: 杂志文章
doi:10.1128/JCM.35.11.2969-2973.1997
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