Unreliable extended-spectrum beta-lactamase detection in the presence of plasmid-mediated AmpC in Escherichia coli clinical isolates.

abstract：:In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, a...

journal_title：Journal of clinical microbiology

authors： Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel H

更新日期：2007-09-01 00:00:00

abstract：:Fungal infection of the thyroid is rare. Most reported cases have involved Aspergillus, Coccidioides, and Candida species in the setting of disseminated disease. Infection of the thyroid with Histoplasma capsulatum is rarely reported as part of disseminated disease, even in geographic areas where histoplasmosis is end...

journal_title：Journal of clinical microbiology

authors： Goldani LZ,Klock C,Diehl A,Monteiro AC,Maia AL

更新日期：2000-10-01 00:00:00

abstract：:The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E...

journal_title：Journal of clinical microbiology

authors： Han J,Swan DC,Smith SJ,Lum SH,Sefers SE,Unger ER,Tang YW

更新日期：2006-11-01 00:00:00

abstract：:Haemophilus influenzae type e biotype 4 was isolated from a single antemortem blood culture obtained from a 60-year-old, white male with abdominal carcinomatosis. ...

journal_title：Journal of clinical microbiology

authors： Cawthern TH,Marraro RV,McCleskey FK

更新日期：1978-01-01 00:00:00

abstract：:The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on ...

journal_title：Journal of clinical microbiology

authors： Hofmann-Thiel S,Molodtsov N,Antonenka U,Hoffmann H

更新日期：2016-12-01 00:00:00

abstract：:Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

journal_title：Journal of clinical microbiology

authors： Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

更新日期：1993-02-01 00:00:00

abstract：:A VIM metallo-beta-lactamase-producing Aeromonas hydrophila strain carrying an integron-borne bla(VIM-4) gene was isolated from a cirrhotic patient's fecal sample in a Budapest hospital. The variable region of this integron is identical with that of a previously characterized integron from Pseudomonas aeruginosa clini...

journal_title：Journal of clinical microbiology

authors： Libisch B,Giske CG,Kovács B,Tóth TG,Füzi M

更新日期：2008-05-01 00:00:00

abstract：:Many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. DiversiLab (DL; bioMérieux) potentially overcomes these limitations. In this study, we evaluated the DL system for the identification of hospital outbreaks of a number bacterial species. Appropriately typed clinica...

journal_title：Journal of clinical microbiology

authors： Fluit AC,Terlingen AM,Andriessen L,Ikawaty R,van Mansfeld R,Top J,Cohen Stuart JW,Leverstein-van Hall MA,Boel CH

更新日期：2010-11-01 00:00:00

abstract：:Four human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43) are associated with a range of respiratory outcomes, including bronchiolitis and pneumonia. Their epidemiologies and clinical characteristics are poorly described and are often reliant on case reports. To address these problems, we conducted a l...

journal_title：Journal of clinical microbiology

authors： Gaunt ER,Hardie A,Claas EC,Simmonds P,Templeton KE

更新日期：2010-08-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:Rapid identification of pathogens from blood cultures can decrease lengths of stay and improve patient outcomes. We evaluated the accuracy of the Verigene Gram-positive blood culture (BC-GP) nucleic acid test for investigational use only (Nanosphere, Inc., Northbrook, IL) for the identification of Gram-positive bacter...

journal_title：Journal of clinical microbiology

authors： Wojewoda CM,Sercia L,Navas M,Tuohy M,Wilson D,Hall GS,Procop GW,Richter SS

更新日期：2013-07-01 00:00:00

abstract：:We have developed a novel and practical DNA-RNA hybridization assay for the detection and identification of Campylobacter pylori in the gastric mucosa. This technique utilizes a [32P]ddATP-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in C. pylori 16S rRNA. This probe is very s...

journal_title：Journal of clinical microbiology

authors： Morotomi M,Hoshina S,Green P,Neu HC,LoGerfo P,Watanabe I,Mutai M,Weinstein IB

更新日期：1989-12-01 00:00:00

abstract：:During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Vibits H,Ursing J,Korner B

更新日期：1991-05-01 00:00:00

abstract：:Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

journal_title：Journal of clinical microbiology

authors： Cosentino LA,Landers DV,Hillier SL

更新日期：2003-08-01 00:00:00

abstract：:Helicobacter pylori virulence determinants have not previously been studied in detail in Latin Americans with H. pylori infections. We characterized the vacA (vacuolating cytotoxin gene A) and cagA (cytotoxin-associated gene A) types of more than 400 single-colony isolates from 20 patients in Mexico City. For 17 patie...

journal_title：Journal of clinical microbiology

authors： Morales-Espinosa R,Castillo-Rojas G,Gonzalez-Valencia G,Ponce de León S,Cravioto A,Atherton JC,López-Vidal Y

更新日期：1999-09-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:Real-time PCR methodology can be applied to rapidly and accurately detect influenza viruses. During times of surge testing or enhanced pandemic surveillance, public health laboratories (PHLs) may experience overwhelming demand for testing, even while the prevalence of positive specimens remains low. To improve laborat...

journal_title：Journal of clinical microbiology

authors： Van TT,Miller J,Warshauer DM,Reisdorf E,Jernigan D,Humes R,Shult PA

更新日期：2012-03-01 00:00:00

abstract：:Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Ijdo JW,Padula SJ,Flavell RA,Fikrig E

更新日期：2000-05-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...

journal_title：Journal of clinical microbiology

authors： Jayarao BM,Doré JJ Jr,Oliver SP

更新日期：1992-09-01 00:00:00

abstract：:To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...

journal_title：Journal of clinical microbiology

authors： Rivera E,López-Vidal Y,Luqueño V,Ruiz-Palacios GM

更新日期：1991-08-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:Humoral immune response to intestinal Rhodococcus (Corynebacterium) equi in horses was studied by enzyme-linked immunosorbent assay. Anti-R. equi immunoglobulin M (IgM), IgG, and IgA antibodies were demonstrated in the healthy horse population. Adult horse levels of anti-R. equi IgM and IgG antibodies were reached by ...

journal_title：Journal of clinical microbiology

authors： Takai S,Kawazu S,Tsubaki S

更新日期：1986-05-01 00:00:00

abstract：:The frequencies of 10 opportunistic DNA viruses were determined by multiplex real-time PCR in paired cerebrospinal fluid (CSF) and brain tissue of HIV-infected individuals. In the CSF, viruses were detectable in 45/55 cases: JC virus (JCV) in 62%, Epstein-Barr virus (EBV) in 44%, cytomegalovirus (CMV) in 25%, varicell...

journal_title：Journal of clinical microbiology

authors： Kannangai R,Sachithanandham J,Mahadevan A,Abraham AM,Sridharan G,Desai A,Ravi V,Shankar SK

更新日期：2013-03-01 00:00:00

abstract：:Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

journal_title：Journal of clinical microbiology

authors： Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

更新日期：2020-03-25 00:00:00

abstract：:The G and P types of 2,912 rotavirus-positive fecal specimens collected from eight geographical areas of the United Kingdom between 1995 and 1998 were determined by reverse transcription-PCR. Although 15 different G-P combinations were identified, G1P[8], G2P[4], G3P[8], and G4P[8] strains constituted 95% of all the r...

journal_title：Journal of clinical microbiology

authors： Iturriza-Gómara M,Green J,Brown DW,Ramsay M,Desselberger U,Gray JJ

更新日期：2000-12-01 00:00:00

abstract：:To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

journal_title：Journal of clinical microbiology

authors： Dimitrov DH,Melnick JL,Hollinger FB

更新日期：1990-04-01 00:00:00

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agg...

journal_title：Journal of clinical microbiology

authors： Smits HL,van der Hoorn MA,Goris MG,Gussenhoven GC,Yersin C,Sasaki DM,Terpstra WJ,Hartskeerl RA

更新日期：2000-03-01 00:00:00