Abstract:
:Prion diseases of cattle include the classical bovine spongiform encephalopathy (C-BSE) and the atypical H-type BSE (H-BSE) and L-type BSE (L-BSE) strains. Although the C- and L-BSE strains can be detected and discriminated by ultrasensitive real-time quaking-induced conversion (RT-QuIC) assays, no such test has yet been described for the detection of H-BSE or the discrimination of each of the major bovine prion strains. Here, we demonstrate an RT-QuIC assay for H-BSE that can detect as little as 10(-9) dilutions of brain tissue and neat cerebrospinal fluid samples from clinically affected cattle. Moreover, comparisons of the reactivities with different recombinant prion protein substrates and/or immunoblot band profiles of proteinase K-treated RT-QuIC reaction products indicated that H-, L-, and C-BSE have distinctive prion seeding activities and can be discriminated by RT-QuIC on this basis.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Masujin K,Orrú CD,Miyazawa K,Groveman BR,Raymond LD,Hughson AG,Caughey Bdoi
10.1128/JCM.02731-15subject
Has Abstractpub_date
2016-03-01 00:00:00pages
676-86issue
3eissn
0095-1137issn
1098-660Xpii
JCM.02731-15journal_volume
54pub_type
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