Species-specific monoclonal antibodies to Rickettsia japonica, a newly identified spotted fever group rickettsia.

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:Ewingella americana was isolated from two blood cultures from a 75-year-old male after cholecystectomy. The characteristics of this strain are compared with the reported biochemical characteristics of 44 American strains. Previously described infections and pseudoinfections with E. americana are reviewed. ...

journal_title：Journal of clinical microbiology

authors： Devreese K,Claeys G,Verschraegen G

更新日期：1992-10-01 00:00:00

abstract：:Accurate identification of pathogenic species is important for early appropriate patient management, but growing diversity of infectious species/strains makes the identification of clinical yeasts increasingly difficult. Among conventional methods that are commercially available, the API ID32C, AuxaColor, and Vitek 2 ...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Efremov L,Leoncini E,Amore R,Posteraro P,Ricciardi W,Sanguinetti M

更新日期：2015-08-01 00:00:00

abstract：:Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...

journal_title：Journal of clinical microbiology

authors： Jaworski JP,Pluta A,Rola-Łuszczak M,McGowan SL,Finnegan C,Heenemann K,Carignano HA,Alvarez I,Murakami K,Willems L,Vahlenkamp TW,Trono KG,Choudhury B,Kuźmak J

更新日期：2018-06-25 00:00:00

abstract：:The importance of euparal as a permanent mounting medium for eggs of parasites and the process of making permanent slides are analyzed and discussed in detail. Studies show that helminth eggs mounted in euparal exhibit excellent optical and drying properties. Euparal also can be used to identify proglottids of Taenia ...

journal_title：Journal of clinical microbiology

authors： Berlin OG,Miller MJ

更新日期：1980-11-01 00:00:00

abstract：:Using the established commercial system Sherlock (MIDI, Inc.), cellular fatty acid methyl ester analysis for differentiation among Burkholderia cepacia complex species was proven. The identification key based on the diagnostic fatty acids is able to discern phenotypically related Ralstonia pickettii and Pandoraea spp....

journal_title：Journal of clinical microbiology

authors： Krejcí E,Kroppenstedt RM

更新日期：2006-03-01 00:00:00

abstract：:To investigate whether both tissue culture and PCR on a sequence from the repetitive rDNA could contribute to the diagnosis of toxoplasmosis, blood samples and, if they were available, cerebrospinal fluid (CSF) and aqueous humor samples from 72 human immunodeficiency virus-seropositive patients with suspected toxoplas...

journal_title：Journal of clinical microbiology

authors： Dupon M,Cazenave J,Pellegrin JL,Ragnaud JM,Cheyrou A,Fischer I,Leng B,Lacut JY

更新日期：1995-09-01 00:00:00

abstract：:A comparative study of herpes simplex virus diagnosis by standard cell culture and a new hybrid test (enzyme-linked immunosorbent assay spin amplification technique) was done on 300 specimens. The new test was found to be equally sensitive and specific, much less expensive to perform, and to report all results in 48 h...

journal_title：Journal of clinical microbiology

authors： Michalski FJ,Shaikh M,Sahraie F,Desai S,Verano L,Vallabhaneni J

更新日期：1986-08-01 00:00:00

abstract：:Rotavirus G and P types from 716 children with acute diarrhea in Dijon from 1995 to 1998 and throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed by P[8],G4, which emerged during the last winter. G9 and P[6] strains were detected at low frequencies...

journal_title：Journal of clinical microbiology

authors： Bon F,Fromantin C,Aho S,Pothier P,Kohli E

更新日期：2000-04-01 00:00:00

abstract：:To provide more sensitive and convenient methods for the detection of equine infectious anemia virus (EIAV), we developed an enzyme-linked immunosorbent assay (ELISA) employing the EIAV gag precursor (Pr55gag) produced by using recombinant DNA techniques. The antigenic reactivity of the recombinant EIAV Pr55gag was fo...

journal_title：Journal of clinical microbiology

authors： Archambault D,Wang ZM,Lacal JC,Gazit A,Yaniv A,Dahlberg JE,Tronick SR

更新日期：1989-06-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:A genotyping study of 285 Hybrid Capture 2 low-risk probe cocktail-positive specimens showed cross-reactivity with several untargeted human papillomavirus genotypes. Cross-reactivity was often clinically beneficial due to the detection of untargeted low-risk genotypes. A total of 8.4% of positive results, usually weak...

journal_title：Journal of clinical microbiology

authors： Poljak M,Kocjan BJ,Kovanda A,Lunar MM,Lepej SZ,Planinic A,Seme K,Vince A

更新日期：2009-08-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:The comparison of Gram-stained urethral smears with Gen-Probe for the detection of Neisseria Gonorrhoeae in the urethras of males with symptomatic urethritis revealed a 99.6% correlation between the two methods. A simple Gram stain would appear to be the method of choice for the detection of gonorrhea in symptomatic m...

journal_title：Journal of clinical microbiology

authors： Juchau SV,Nackman R,Ruppart D

更新日期：1995-11-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:An examination of 45 clinical isolates of Cryptococcus neoformans revealed an unusually high incidence of C. neoformans var. gattii in South Australia (65%) and in the Northern Territory (95%). In assessing all the available data from Australian isolates of C. neoformans, there appeared to be an endemic focus for the ...

journal_title：Journal of clinical microbiology

authors： Ellis DH

更新日期：1987-02-01 00:00:00

abstract：:Low-speed centrifugation-mediated adsorption was evaluated as an enhancement of infectivity of clinical and laboratory strains of cytomegalovirus (CMV) occurring with cells grown in conventional culture tubes. The time required for reporting of primary isolates of CMV from urine specimens adsorbed onto monolayers of W...

journal_title：Journal of clinical microbiology

authors： Oefinger PE,Shawar RM,Loo SH,Tsai LT,Arnett JK

更新日期：1990-05-01 00:00:00

abstract：:Comparison of nucleotide sequences of the partial 1D region of foot-and-mouth disease type C viruses of Indian origin with those of European, South American, and Southeast Asian viruses revealed that the Indian viruses form a distinct genotype. The vaccine strain C IND/51/79 belongs to this genotype and may be a proto...

journal_title：Journal of clinical microbiology

authors： Nagendrakumar SB,Reddy GS,Chandran D,Thiagarajan D,Rangarajan PN,Srinivasan VA

更新日期：2005-02-01 00:00:00

abstract：:We have developed a rapid and sensitive assay for the detection of Salmonella serovars in veterinary clinical specimens. This method utilizes a short cultivation period followed by PCR. For detection of the amplified product, an enzyme-linked immunosorbent assay (ELISA)-based oligonucleotide ligation assay (OLA) was u...

journal_title：Journal of clinical microbiology

authors： Stone GG,Oberst RD,Hays MP,McVey S,Chengappa MM

更新日期：1995-11-01 00:00:00

abstract：:INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Alby K

更新日期：2018-03-26 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...

journal_title：Journal of clinical microbiology

authors： Kenny MT,Brackman MA

更新日期：1994-05-01 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:Clinical evidence for failure with beta-lactam therapy has been lacking for patients with borderline oxacillin-resistant Staphylococcus aureus (BORSA) infections. We describe a failure of cloxacillin for a patient with endocarditis due to BORSA. The isolate also had false-negative thermonuclease and coagulase test res...

journal_title：Journal of clinical microbiology

authors： Skinner S,Murray M,Walus T,Karlowsky JA

更新日期：2009-03-01 00:00:00

abstract：:We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

journal_title：Journal of clinical microbiology

authors： Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

更新日期：2007-06-01 00:00:00

abstract：:In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

journal_title：Journal of clinical microbiology

authors： La Scolea LJ Jr,Keddell JE

更新日期：1981-04-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that has been responsible for major nosocomial epidemics worldwide. For infection control programs, rapid and adequate detection of MRSA is of great importance. We developed a rapid and high-throughput molecular screening approach that consist...

journal_title：Journal of clinical microbiology

authors： Nijhuis RH,van Maarseveen NM,van Hannen EJ,van Zwet AA,Mascini EM

更新日期：2014-08-01 00:00:00

abstract：:Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens...

journal_title：Journal of clinical microbiology

authors： Dare R,Sanghavi S,Bullotta A,Keightley MC,George KS,Wadowsky RM,Paterson DL,McCurry KR,Reinhart TA,Husain S,Rinaldo CR

更新日期：2007-02-01 00:00:00

abstract：:Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...

journal_title：Journal of clinical microbiology

authors： Farber JM,Speirs JI

更新日期：1987-08-01 00:00:00

abstract：:A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984....

journal_title：Journal of clinical microbiology

authors： Ukkonen P,Huovilainen A,Hovi T

更新日期：1986-12-01 00:00:00