Cryptococcus neoformans var. gattii in Australia.

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:Ovine progressive pneumonia virus (OPPV) is a lentivirus which causes a progressive disease in sheep. Immunodominant epitopes have been identified in the envelope gp40 glycoprotein. Synthetic peptides representing these regions are able to detect the presence of OPPV antibodies in 96% of infected sheep. ...

journal_title：Journal of clinical microbiology

authors： Kwang J,Torres JV

更新日期：1994-07-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:A mycobacterium isolated from a clinical sample of an AIDS patient was identified as Mycobacterium interjectum by direct 16S rRNA sequence determination. High-performance liquid chromatography, however, revealed a mycolic acid pattern which was different from the one shared by the previously analyzed strains of this s...

journal_title：Journal of clinical microbiology

authors： Tortoli E,Kirschner P,Bartoloni A,Burrini C,Manfrin V,Mantella A,Scagnelli M,Scarparo C,Simonetti MT,Böttger EC

更新日期：1996-09-01 00:00:00

abstract：:The etiologic role of oral treponemes in human periodontitis is still under debate. Although seen by dark-field microscopy in large numbers, their possible role is still unclear since they comprise some 60 different phylotypes, most of which are still uncultured. To determine their status as mere commensals or opportu...

journal_title：Journal of clinical microbiology

authors： Moter A,Riep B,Haban V,Heuner K,Siebert G,Berning M,Wyss C,Ehmke B,Flemmig TF,Göbel UB

更新日期：2006-09-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains ...

journal_title：Journal of clinical microbiology

authors： Smith JA,Ngui-Yen JH

更新日期：1980-07-01 00:00:00

abstract：:The etiology of the purple urine bag syndrome (PUBS), in which the urinary catheter bag of some elderly patients develops intense purple coloration, was studied. The purple was found to be a mixture of indirubin dissolved in the plastic and indigo on its surface. Six patients with PUBS were studied, and Providencia st...

journal_title：Journal of clinical microbiology

authors： Dealler SF,Hawkey PM,Millar MR

更新日期：1988-10-01 00:00:00

abstract：:A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA

更新日期：1984-06-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with r...

journal_title：Journal of clinical microbiology

authors： Fenollar F,Fournier PE,Robert C,Raoult D

更新日期：2004-01-01 00:00:00

abstract：:Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as ...

journal_title：Journal of clinical microbiology

authors： Alvarez E,Garcia-Hermoso D,Sutton DA,Cano JF,Stchigel AM,Hoinard D,Fothergill AW,Rinaldi MG,Dromer F,Guarro J

更新日期：2010-12-01 00:00:00

abstract：:To objectively assess the value of examining multiple sputum specimens in maximizing the sensitivity of detection of Mycobacterium tuberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients diagnosed with culture-proven pulmonary tuberculosis (TB) at Hennepin County Medical ...

journal_title：Journal of clinical microbiology

authors： Nelson SM,Deike MA,Cartwright CP

更新日期：1998-02-01 00:00:00

abstract：:Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques. Results from the LCR assay (Abbott LCx Mycobacterium tuberculosis [MTB] Assay) were compared to results from standard cultur...

journal_title：Journal of clinical microbiology

authors： Moore DF,Curry JI

更新日期：1998-04-01 00:00:00

abstract：:Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

journal_title：Journal of clinical microbiology

authors： Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

更新日期：1996-11-01 00:00:00

abstract：:A multiwell, dried antimicrobial agent susceptibility test system, Sceptor (BBL Microbiology Systems, Cockeysville, Md.), was tested. The system was compared directly with a reference microdilution method by using two collections of stock cultures and 305 fresh clinical isolates. Sceptor was found to be in agreement (...

journal_title：Journal of clinical microbiology

authors： Jones RN,Thornsberry C,Barry AL,Gavan TL

更新日期：1981-01-01 00:00:00

abstract：:This study describes the molecular identification of 520 Entamoeba-positive fecal samples from a large and diverse population of captive nonhuman primates (NHP). The results revealed the presence of Entamoeba histolytica (NHP variant only), E. dispar, E. moshkovskii, E. hartmanni, E. coli, and E. polecki-like organism...

journal_title：Journal of clinical microbiology

authors： Levecke B,Dreesen L,Dorny P,Verweij JJ,Vercammen F,Casaert S,Vercruysse J,Geldhof P

更新日期：2010-08-01 00:00:00

abstract：:A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

journal_title：Journal of clinical microbiology

authors： Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

更新日期：2002-12-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:The AutoSCAN-3 (American MicroScan, Mahwah, N.J.) is an instrument capable of automated reading of commercially available microdilution trays for identification and quantitative susceptibility testing of rapidly growing bacteria. This study compared the results of visual and automated reading of microdilution trays fo...

journal_title：Journal of clinical microbiology

authors： DeGirolami PC,Eichelberger KA,Salfity LC,Rizzo MF

更新日期：1983-12-01 00:00:00

abstract：:The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

journal_title：Journal of clinical microbiology

authors： Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

更新日期：2002-08-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...

journal_title：Journal of clinical microbiology

authors： Cha TA,Kolberg J,Irvine B,Stempien M,Beall E,Yano M,Choo QL,Houghton M,Kuo G,Han JH

更新日期：1991-11-01 00:00:00

abstract：:A 42-year-old woman developed severe, recurrent breast abscesses caused by Corynebacterium minutissimum. Prior reports of C. minutissimum infection have been limited to erythrasma, a minor dermatosis. The microbiological and clinical features of this species were reviewed. ...

journal_title：Journal of clinical microbiology

authors： Berger SA,Gorea A,Stadler J,Dan M,Zilberman M

更新日期：1984-12-01 00:00:00

abstract：:Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nas...

journal_title：Journal of clinical microbiology

authors： Greiner O,Day PJ,Bosshard PP,Imeri F,Altwegg M,Nadal D

更新日期：2001-09-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the dis...

journal_title：Journal of clinical microbiology

authors： Olsen RG,Blakeslee JR,Mathes L,Nakano JH

更新日期：1977-07-01 00:00:00

abstract：:Blood culture contamination greatly affects clinical decisions. Hence, it is of interest to assess the influence of factors such as the volume of blood drawn and the site of blood draw on the rates of blood culture contamination. In a retrospective study, blood cultures from infants and children up to 18 years of age ...

journal_title：Journal of clinical microbiology

authors： Gonsalves WI,Cornish N,Moore M,Chen A,Varman M

更新日期：2009-11-01 00:00:00

abstract：:The p1 genes of 60 Mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. An AGT trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. In addition, a novel p1 gene variant named 2c was identified in ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Cao B,Li J,Song S,Tao X,Yin Y,He L,Zhang J

更新日期：2011-08-01 00:00:00