Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:In anticipation of rotavirus vaccine introduction in Saudi Arabia, this study was undertaken to determine the distribution of the G and P genotypes of rotaviruses in order to examine whether there was any emerging serotype or unusual strain circulating in children in Saudi Arabia. Of 984 stool specimens collected betw...

journal_title：Journal of clinical microbiology

authors： Kheyami AM,Nakagomi T,Nakagomi O,Dove W,Hart CA,Cunliffe NA

更新日期：2008-04-01 00:00:00

abstract：:A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dep...

journal_title：Journal of clinical microbiology

authors： Pennell DR,Wand PJ,Schell RF

更新日期：1987-11-01 00:00:00

abstract：:The influence of 0.05% sodium polyanethol sulfonate on the growth of 24 strains of Neisseria meningitidis in broth medium was examined; several of the strains were markedly inhibited. A paper disk method was evolved for screening the sensitivity of various bacteria to sodium polyanethol sulfonate on solid medium; the ...

journal_title：Journal of clinical microbiology

authors： Eng J,Iveland H

更新日期：1975-05-01 00:00:00

abstract：:The aim of the present study was to evaluate the Helicobacter pylori ClariRes assay (Ingenetix, Vienna, Austria) for the detection of H. pylori infection and the simultaneous clarithromycin susceptibility testing of the H. pylori isolates in stool samples from 100 symptomatic children. The results obtained by this nov...

journal_title：Journal of clinical microbiology

authors： Lottspeich C,Schwarzer A,Panthel K,Koletzko S,Rüssmann H

更新日期：2007-06-01 00:00:00

abstract：:Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...

journal_title：Journal of clinical microbiology

authors： Betrán A,Rezusta A,Lezcano MA,Villuendas MC,Revillo MJ,Boiron P,Rodríguez-Nava V

更新日期：2009-06-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis is shed into the milk and feces of cows with advanced Johne's disease, allowing the transmission of M. avium subsp. paratuberculosis between animals. The objective of this study was to formulate an optimized protocol for the isolation of M. avium subsp. paratuberculosis in m...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-07-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:The results of PCR-based molecular typing of Helicobacter pylori strains by restriction fragment length polymorphism analysis of a 1, 161-bp nucleotide sequence of the midregion of the vacA gene are reported. A total of 48 H. pylori strains isolated from gastric biopsy specimens obtained from 18 patients with peptic u...

journal_title：Journal of clinical microbiology

authors： Donati M,Storni E,D'Apote L,Moreno S,Tucci A,Poli L,Cevenini R

更新日期：1999-04-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

journal_title：Journal of clinical microbiology

authors： Wang G,van Dam AP,Spanjaard L,Dankert J

更新日期：1998-03-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:Resistance to linezolid is rare in clinical isolates of Enterococcus faecalis. A strain resistant to this antimicrobial but susceptible to vancomycin was found to cause central venous catheter colonization in a patient who never received linezolid. ...

journal_title：Journal of clinical microbiology

authors： Marra AR,Major Y,Edmond MB

更新日期：2006-05-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B su...

journal_title：Journal of clinical microbiology

authors： Zhang W,Bielaszewska M,Kuczius T,Karch H

更新日期：2002-04-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:A total of 30 isolates of Treponema hyodysenteriae collected in the Saint-Hyacinthe (Quebec, Canada) area were serotyped by agar gel double immunodiffusion by using extracted lipopolysaccharide and hyperimmune rabbit antisera. Only 17% (5 of 30) of the isolates were typed with antisera specific for each of the seven k...

journal_title：Journal of clinical microbiology

authors： Li ZS,Bélanger M,Jacques M

更新日期：1991-12-01 00:00:00

abstract：:Until recently, the laboratory diagnosis of central nervous system (CNS) infections with herpes simplex virus (HSV) has been limited by poor sensitivity and/or specificity. We assessed the diagnostic utility of PCR for detection of HSV in over 2,100 specimens referred to the Mayo Clinic from August 1993 to May 1996. D...

journal_title：Journal of clinical microbiology

authors： Mitchell PS,Espy MJ,Smith TF,Toal DR,Rys PN,Berbari EF,Osmon DR,Persing DH

更新日期：1997-11-01 00:00:00

abstract：:Autopsy specimens of lung tissues from 15 patients that contracted legionellosis during the 1976 Philadelphia outbreak of Legionnaires disease were examined for the presence of Legionella organisms and soluble antigens by indirect fluorescent-antibody (IFA) testing and by an enzyme-linked immunosorbent assay (ELISA) w...

journal_title：Journal of clinical microbiology

authors： Brown SL,Bibb WF,McKinney RM

更新日期：1985-01-01 00:00:00

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:Rotavirus G and P types from 716 children with acute diarrhea in Dijon from 1995 to 1998 and throughout France during the winter of 1997-1998 were analyzed by reverse transcription-PCR. P[8],G1 predominated, followed by P[8],G4, which emerged during the last winter. G9 and P[6] strains were detected at low frequencies...

journal_title：Journal of clinical microbiology

authors： Bon F,Fromantin C,Aho S,Pothier P,Kohli E

更新日期：2000-04-01 00:00:00

abstract：:The detection and identification of intestinal helminths were studied retrospectively by comparing the Formalin concentration technique with the trichrome-stained smear technique. A total of 3,997 stool samples from 1,570 patients were examined by both methods. Of the 3,997 samples, 31% (1,239 of 3,997) contained helm...

journal_title：Journal of clinical microbiology

authors： Wood JC,Friedly G,de la Maza LM

更新日期：1982-12-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00

abstract：:The establishment of an optimal noninvasive method for diagnosing invasive aspergillosis (IA) is needed to improve the management of this life-threatening infection in patients with hematological disorders, and a number of noninvasive tests for IA that target different fungal components, including galactomannan, (1-->...

journal_title：Journal of clinical microbiology

authors： Kawazu M,Kanda Y,Nannya Y,Aoki K,Kurokawa M,Chiba S,Motokura T,Hirai H,Ogawa S

更新日期：2004-06-01 00:00:00

abstract：:An outbreak of folliculitis caused by Pseudomonas aeruginosa serotype O:7 occurred among the guests of a hotel in St. John's, Newfoundland, Canada, and the source of the infection was traced to the hotel whirlpool. Of 36 persons who used the whirlpool, 26 (72%) developed folliculitis within 1 to 5 days after exposure;...

journal_title：Journal of clinical microbiology

authors： Ratnam S,Hogan K,March SB,Butler RW

更新日期：1986-03-01 00:00:00

abstract：:A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

journal_title：Journal of clinical microbiology

authors： Beaucage CM,Onderdonk AB

更新日期：1982-09-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

journal_title：Journal of clinical microbiology

authors： Landman D,Salamera J,Quale J

更新日期：2013-12-01 00:00:00

abstract：:An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over a 4-week period. Four patients developed C. albicans bloodstream infections. An additional patient developed catheter-related C. albicans infection; the sixth patient developed an infection of cerebrospi...

journal_title：Journal of clinical microbiology

authors： Voss A,Pfaller MA,Hollis RJ,Rhine-Chalberg J,Doebbeling BN

更新日期：1995-03-01 00:00:00