Isolation of an unusual mycobacterium from an AIDS patient.

abstract：:The BBL Crystal system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a miniaturized bacterial identification method employing modified conventional and chromogenic substrates. Two products are currently available, the Rapid Stool/Enteric ID Kit and the Enteric/Nonfermenter ID Kit, each comprising thirt...

journal_title：Journal of clinical microbiology

authors： Holmes B,Costas M,Thaker T,Stevens M

更新日期：1994-09-01 00:00:00

abstract：:The genus Corynebacterium is a heterogeneous group of species comprising human and animal pathogens and environmental bacteria. It is defined on the basis of several phenotypic characters and the results of DNA-DNA relatedness and, more recently, 16S rRNA gene sequencing. However, the 16S rRNA gene is not polymorphic ...

journal_title：Journal of clinical microbiology

authors： Khamis A,Raoult D,La Scola B

更新日期：2004-09-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

journal_title：Journal of clinical microbiology

authors： Fridriksdóttir V,Nesse LL,Gudding R

更新日期：1992-05-01 00:00:00

abstract：:Panels of hepatitis B virus surface antigen-positive sera from drug abusers were used to evaluate 14 commercial enzyme immunoassays from six companies for detecting hepatitis delta virus (HDV) markers. For detecting hepatitis delta virus antigen (HDAg), the Wellcome, Pasteur and Noctech assays had 100% sensitivity for...

journal_title：Journal of clinical microbiology

authors： Shattock AG,Morris MC

更新日期：1991-09-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:Polyomavirus BK (BKV) establishes latent infection in various human tissues, including the kidney. Reactivation following renal transplantation (RT) may cause BKV-associated nephropathy, leading to graft loss. BKV reactivation is often associated with extensive rearrangements in the BKV noncoding regulatory region (NC...

journal_title：Journal of clinical microbiology

authors： Perets TT,Silberstein I,Rubinov J,Sarid R,Mendelson E,Shulman LM

更新日期：2009-05-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00

abstract：:Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

journal_title：Journal of clinical microbiology

authors： Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

更新日期：2005-08-01 00:00:00

abstract：:Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay ...

journal_title：Journal of clinical microbiology

authors： Poli G,Stott J,Liu YS,Manning JS

更新日期：1982-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood from septic patients was examined. Oligonucleotide probes, fluorescently labeled with fluorescein isothiocyanate, directed against the variable regions of the 16S rRNAs of the following bacterial species and/or genera ...

journal_title：Journal of clinical microbiology

authors： Jansen GJ,Mooibroek M,Idema J,Harmsen HJ,Welling GW,Degener JE

更新日期：2000-02-01 00:00:00

abstract：:Serological interrelations among 46 strains of Haemophilus somnus of various geographical and pathological origins were studied by agglutination and agglutinin adsorption procedures. Sera prepared against nine representative American and Swiss isolates agglutinated all other strains to various titers. Adsorption of an...

journal_title：Journal of clinical microbiology

authors： Canto GJ,Biberstein EL

更新日期：1982-06-01 00:00:00

abstract：:Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Matsuda Y,Seki R,Tsubokura M,Takeda N,Shubin FN,Paik IK,Zheng XB

更新日期：2001-10-01 00:00:00

abstract：:By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

journal_title：Journal of clinical microbiology

authors： Osaki T,Taguchi H,Yamaguchi H,Kamiya S

更新日期：1998-01-01 00:00:00

abstract：:Two extended-spectrum beta-lactamase (ESBL)-producing salmonella isolates, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium, were analyzed. Both isolates produced the CTX-M-3 ESBL; however, their bla(CTX-M-3) genes were located on different plasmids. The serovar Typhimurium isolate a...

journal_title：Journal of clinical microbiology

authors： Baraniak A,Sadowy E,Hryniewicz W,Gniadkowski M

更新日期：2002-03-01 00:00:00

abstract：:Better detection of minority human immunodeficiency virus type 1 (HIV-1) populations containing gene mutations may improve the usefulness of antiretroviral resistance testing for clinical management. Molecular cloning of HIV-1 PCR products which might improve minority detection can be slow and difficult, and commercia...

journal_title：Journal of clinical microbiology

authors： Martinez-Picado J,Sutton L,De Pasquale MP,Savara AV,D'Aquila RT

更新日期：1999-09-01 00:00:00

abstract：:Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE tria...

journal_title：Journal of clinical microbiology

authors： Ssengooba W,Iragena JD,Nakiyingi L,Mujumbi S,Wobudeya E,Mboizi R,Boulware D,Meya DB,Choo L,Crook AM,Lebeau K,Joloba M,Demers AM,Cresswell FV,Gibb DM

更新日期：2020-08-24 00:00:00

abstract：:The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

journal_title：Journal of clinical microbiology

authors： Katayama Y,Robinson DA,Enright MC,Chambers HF

更新日期：2005-05-01 00:00:00

abstract：:Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Le TA,Fabre L,Roumagnac P,Grimont PA,Scavizzi MR,Weill FX

更新日期：2007-11-01 00:00:00

abstract：:We have developed a PCR assay for one-step differentiation of the three complexes of New World Leishmania (Leishmania braziliensis, Leishmania mexicana, and Leishmania donovani). This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these organisms and can detect all three complexes si...

journal_title：Journal of clinical microbiology

authors： Harris E,Kropp G,Belli A,Rodriguez B,Agabian N

更新日期：1998-07-01 00:00:00

abstract：:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). ...

journal_title：Journal of clinical microbiology

authors： Twin J,Taylor N,Garland SM,Hocking JS,Walker J,Bradshaw CS,Fairley CK,Tabrizi SN

更新日期：2011-03-01 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX...

journal_title：Journal of clinical microbiology

authors： al Naiemi N,Bart A,de Jong MD,Vandenbroucke-Grauls CM,Rietra PJ,Debets-Ossenkopp YJ,Wever PC,Spanjaard L,Bos AJ,Duim B

更新日期：2006-08-01 00:00:00

abstract：:The FUNGITEST method (Sanofi Diagnostics Pasteur, Paris, France) is a microplate-based procedure for the breakpoint testing of six antifungal agents (amphotericin B, flucytosine, fluconazole, itraconazole, ketoconazole, and miconazole). We compared the FUNGITEST method with a broth microdilution test, performed accord...

journal_title：Journal of clinical microbiology

authors： Davey KG,Holmes AD,Johnson EM,Szekely A,Warnock DW

更新日期：1998-04-01 00:00:00

abstract：:Polyomavirus BK (BKV) causes asymptomatic latent infection in the human host that is reactivated during periods of immune suppression. Detection by conventional tube cell culture is difficult and time consuming because BKV exhibits slow growth with late (14 to 28 days) and subtle cytopathic effects. We developed a she...

journal_title：Journal of clinical microbiology

authors： Marshall WF,Telenti A,Proper J,Aksamit AJ,Smith TF

更新日期：1990-07-01 00:00:00

abstract：:High-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. Isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees C. After removal of organis...

journal_title：Journal of clinical microbiology

authors： Harpold DJ,Wasilauskas BL

更新日期：1987-06-01 00:00:00

abstract：:Rapid and accurate tests are currently needed to identify individuals with high levels of Loa loa microfilaria (mf), so that these individuals may be excluded from mass ivermectin administration campaigns against onchocerciasis and lymphatic filariasis being conducted in areas where Onchocerca volvulus, Wuchereria ban...

journal_title：Journal of clinical microbiology

authors： Drame PM,Fink DL,Kamgno J,Herrick JA,Nutman TB

更新日期：2014-06-01 00:00:00

abstract：:Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

journal_title：Journal of clinical microbiology

authors： Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

更新日期：1991-02-01 00:00:00