Single-step multiplex PCR assay for characterization of New World Leishmania complexes.

abstract：:Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of...

journal_title：Journal of clinical microbiology

authors： Schiff LJ

更新日期：1976-09-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:Two extended-spectrum beta-lactamase (ESBL)-producing salmonella isolates, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium, were analyzed. Both isolates produced the CTX-M-3 ESBL; however, their bla(CTX-M-3) genes were located on different plasmids. The serovar Typhimurium isolate a...

journal_title：Journal of clinical microbiology

authors： Baraniak A,Sadowy E,Hryniewicz W,Gniadkowski M

更新日期：2002-03-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

journal_title：Journal of clinical microbiology

authors： Sinclair A,Xie X,Teltscher M,Dendukuri N

更新日期：2013-07-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:To assess whether PCR is applicable for monitoring the efficacy of antituberculous treatment, respiratory specimens obtained during treatment and follow-up from sputum smear-positive tuberculosis (TB) patients were examined. First, results of smear, culture, and PCR for Mycobacterium tuberculosis complex (MTB) and an ...

journal_title：Journal of clinical microbiology

authors： Thomsen VO,Kok-Jensen A,Buser M,Philippi-Schulz S,Burkardt HJ

更新日期：1999-11-01 00:00:00

abstract：:The epidemiological study of several multidrug-resistant Enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase TEM-24 from a clonal strain of Enterobacter aerogenes to different strains of Klebsiella pneumoniae, Escherichia c...

journal_title：Journal of clinical microbiology

authors： Neuwirth C,Siebor E,Pechinot A,Duez JM,Pruneaux M,Garel F,Kazmierczak A,Labia R

更新日期：2001-05-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:Paired serum samples from 93 patients suspected of having measles were assayed for measles virus-specific immunoglobulin M (IgM) antibodies by an enzyme immunoassay (EIA), and the results were compared with results from a complement fixation assay and an EIA for measles virus IgG. By using significant serologic rises ...

journal_title：Journal of clinical microbiology

authors： Mayo DR,Brennan T,Cormier DP,Hadler J,Lamb P

更新日期：1991-12-01 00:00:00

abstract：:Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal...

journal_title：Journal of clinical microbiology

authors： Jones KF,Norcross NL

更新日期：1983-05-01 00:00:00

abstract：:Corynebacterium amycolatum has not been reported as a cause of human infections up to now, but usually the bacterium is misidentified in clinical specimens as Corynebacterium xerosis. We report the first case of neonatal sepsis due to Corynebacterium amycolatum with a fatal outcome in a premature infant. ...

journal_title：Journal of clinical microbiology

authors： Berner R,Pelz K,Wilhelm C,Funke A,Leititis JU,Brandis M

更新日期：1997-04-01 00:00:00

abstract：:The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

journal_title：Journal of clinical microbiology

authors： Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

更新日期：2003-04-01 00:00:00

abstract：:Treatment of wild rats from a known scrub typhus focus in the Philippines with cyclophosphamide did not significantly enhance the isolation rate of Rickettsia tsutsugamushi using mouse inoculation techniques. Similarly, cyclophosphamide treatment of mice inoculated with organs of wild rats did not increase the recover...

journal_title：Journal of clinical microbiology

authors： van Peenen PF,Ho CM,See R

更新日期：1977-01-01 00:00:00

abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...

journal_title：Journal of clinical microbiology

authors： Wong JD,Janda JM

更新日期：1992-03-01 00:00:00

abstract：:Four Mycoplasma arthritidis strains, two virulent (158p10p9 and 14124p10) and two avirulent (PG6 and H606), were examined for differences in their antigenic compositions. Rabbit antisera prepared against each strain were compared for their reactivities against both homologous and heterologous strains by enzyme-linked ...

journal_title：Journal of clinical microbiology

authors： Washburn LR,Hirsch S

更新日期：1990-09-01 00:00:00

abstract：:Plasmodium nucleic acids have been detected in serum and plasma, but there is little published data describing the diagnostic performance of malaria nucleic acid amplification tests (NAATs) using these specimen types. Previously, our group described a multiplex NAAT for the detection of dengue virus, Leptospira, and P...

journal_title：Journal of clinical microbiology

authors： Waggoner JJ,Okangba C,Mohamed-Hadley A,Lefterova MI,Banaei N,Oyibo W,Pinsky BA

更新日期：2015-11-01 00:00:00

abstract：:An increased human parvovirus B19 infection rate has been observed in immunocompromised hosts. In this study, we sought to determine the prevalence of parvovirus B19 infection in adult cancer patients receiving multiple courses of systemic chemotherapy. From March 1999 through April 2000, 59 men and 68 women, with a m...

journal_title：Journal of clinical microbiology

authors： Kuo SH,Lin LI,Chang CJ,Liu YR,Lin KS,Cheng AL

更新日期：2002-11-01 00:00:00

abstract：:Eight strains of a new intermediate adenovirus were isolated in the course of an investigation of conjunctivitis in Hiroshima City, Japan. The strain was first isolated in July 1986. All eight strains were isolated from conjunctival swab samples from patients with conjunctivitis, mainly epidemic keratoconjunctivitis. ...

journal_title：Journal of clinical microbiology

authors： Noda M,Miyamoto Y,Ikeda Y,Matsuishi T,Ogino T

更新日期：1991-07-01 00:00:00

abstract：:During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In genera...

journal_title：Journal of clinical microbiology

authors： Goossens H,Pot B,Vlaes L,Van den Borre C,Van den Abbeele R,Van Naelten C,Levy J,Cogniau H,Marbehant P,Verhoef J

更新日期：1990-05-01 00:00:00

abstract：:Nocardia asteroides is a rare cause of keratitis usually associated with trauma. We report a case of corneal ulceration caused by N. asteroides in a patient with leprosy. This is the first case report of nocardial keratitis from Southeast Asia. The diminished corneal sensation in a patient with leprosy could be a pred...

journal_title：Journal of clinical microbiology

authors： Tendolkar UM,Varaiya A,Ahuja AS,Motwane SA,Gogate AS

更新日期：1998-04-01 00:00:00

abstract：:The volume of blood sampled for blood culture determines its sensitivity. We measured low mean blood volumes in submitted aerobic (8.38 ± 3.88 ml) and anaerobic (7.16 ± 3.83 ml) blood culture bottles. Educational seminars were held for phlebotomy teams, and renewed measurements thereafter revealed significantly higher...

journal_title：Journal of clinical microbiology

authors： van Ingen J,Hilt N,Bosboom R

更新日期：2013-03-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00

abstract：:The performance of the Amplified Mycobacterium Tuberculosis Direct (AMTD) test (Gen-Probe Inc., San Diego, Calif.) was assessed in a large tertiary care mycobacteriology laboratory. Both acid-fast smear-positive and smear-negative respiratory and nonrespiratory clinical specimens were analyzed. From February 1998 to 4...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Miller DR,Schneider PS,Roberts GD

更新日期：2002-05-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:The reliability of the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB) for the diagnosis of pulmonary tuberculosis was evaluated by testing 956 respiratory specimens from 502 patients and comparing results with results by culture and medical history. Of those 135 specimens that were culture positive for ...

journal_title：Journal of clinical microbiology

authors： Bergmann JS,Woods GL

更新日期：1996-05-01 00:00:00

abstract：:The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desor...

journal_title：Journal of clinical microbiology

authors： Triest D,Stubbe D,De Cremer K,Piérard D,Normand AC,Piarroux R,Detandt M,Hendrickx M

更新日期：2015-02-01 00:00:00