Predominance of the ac variant in K88-positive Escherichia coli isolates from swine.

abstract：:Norwalk virus (NV) and the Norwalk-like viruses are important human pathogens that cause epidemic acute viral gastroenteritis. Current techniques used to recover NV from clinical samples involve multistep viral extraction and elution procedures with subsequent viral detection by reverse transcription-PCR (RT-PCR). In ...

journal_title：Journal of clinical microbiology

authors： Schwab KJ,Estes MK,Neill FH,Atmar RL

更新日期：1997-02-01 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...

journal_title：Journal of clinical microbiology

authors： Hietala SK,Crossley BM

更新日期：2006-01-01 00:00:00

abstract：:A rapid (2.5 h) direct enzyme immunoassay (EIA) for Clostridium difficile toxin A was developed for clinical use. Specimen centrifugation and filtration were not required. The EIA detected toxin A levels in patient stool as low as 20 pg (2 ng/ml of stool). The test was 5,000 times more sensitive for toxin A than it wa...

journal_title：Journal of clinical microbiology

authors： DiPersio JR,Varga FJ,Conwell DL,Kraft JA,Kozak KJ,Willis DH

更新日期：1991-12-01 00:00:00

abstract：:Conditions were defined for functional covalent coupling of anti-Lassa virus globulins to glutaraldehyde-fixed chicken erythrocytes. Tolylene-2,4-diisocyanate in a reaction mixture containing not more than 0.01 M NaCl produced uniformly good conjugates which were used in reversed passive hemagglutination (RPH) and rev...

journal_title：Journal of clinical microbiology

authors： Goldwasser RA,Elliott LH,Johnson KM

更新日期：1980-06-01 00:00:00

abstract：:Eight commercial enzyme-linked immunosorbent assays (ELISAs) were used to test sera taken from 102 patients in whom Helicobacter pylori infection status had been determined by means of biopsy culture, PCR, histology, and urease production and by 13C urea breath test. By those means, 61 patients had been found to be in...

journal_title：Journal of clinical microbiology

authors： Meijer BC,Thijs JC,Kleibeuker JH,van Zwet AA,Berrelkamp RJ

更新日期：1997-01-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacryla...

journal_title：Journal of clinical microbiology

authors： Saengjaruk P,Chaicumpa W,Watt G,Bunyaraksyotin G,Wuthiekanun V,Tapchaisri P,Sittinont C,Panaphut T,Tomanakan K,Sakolvaree Y,Chongsa-Nguan M,Mahakunkijcharoen Y,Kalambaheti T,Naigowit P,Wambangco MA,Kurazono H,Hayashi H

更新日期：2002-02-01 00:00:00

abstract：:We have developed a single-tube multiplex real-time PCR method for the detection of the eight most common Candida species causing septicemia: Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis. The method developed targets the RNase P RNA gene RP...

journal_title：Journal of clinical microbiology

authors： Innings A,Ullberg M,Johansson A,Rubin CJ,Noreus N,Isaksson M,Herrmann B

更新日期：2007-03-01 00:00:00

abstract：:Yersinia enterocolitica is known to be associated with gastroenteritis in children and Reiter's syndrome in adults, but it has only rarely been the cause of primary soft tissue infections. A patient with diabetes mellitus developed a calf abscess, from which Y. enterocolitica was isolated in pure culture. Incision, dr...

journal_title：Journal of clinical microbiology

authors： Brennessel DJ,Robbins N,Hindman S

更新日期：1984-08-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:Hepatitis C virus (HCV)-positive sera from 106 chronically infected patients which had previously been genotyped were characterized by serotyping. Genotypes were determined by a first-generation line probe assay (INNO-LiPA HCV) and by sequence analyses of the core, core-E1, and NS5B regions. HCV serotypes were determi...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Kleter B,Pike I,Quint W

更新日期：1996-07-01 00:00:00

abstract：:Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...

journal_title：Journal of clinical microbiology

authors： Sherefa K,Sällberg M,Johansson B,Salminen M,Sönnerborg A

更新日期：1997-09-01 00:00:00

abstract：:We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

journal_title：Journal of clinical microbiology

authors： Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

更新日期：1996-11-01 00:00:00

abstract：:This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA ins...

journal_title：Journal of clinical microbiology

authors： Iversen J,Stendal G,Gerdes CM,Meyer CH,Andersen CØ,Frimodt-Møller N

更新日期：2016-02-01 00:00:00

abstract：:A washing procedure that removed maximum numbers of contaminating microorganisms from whole surgical gloves was developed. Washing, coupled with membrane filtration, proved to be a simple and effective method for bioburden determinations on whole gloves. ...

journal_title：Journal of clinical microbiology

authors： Lammerding AM,Day DF

更新日期：1978-05-01 00:00:00

abstract：:Polyclonal anti-idiotypic antibodies (anti-Ids) were generated by the sequential immunization of rabbits with three mouse monoclonal antibodies (MAb1s) specific for a major bluetongue virus (BTV) protein, VP7. The anti-Ids, designated RAb2s, recognized idiotopes which were located within or near the antigen-combining ...

journal_title：Journal of clinical microbiology

authors： Zhou EM,Huang W

更新日期：1995-04-01 00:00:00

abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...

journal_title：Journal of clinical microbiology

authors： Levine M,Beck I,Styrchak S,Pepper G,Frenkel L

更新日期：2016-07-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

journal_title：Journal of clinical microbiology

authors： Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

更新日期：2005-02-01 00:00:00

abstract：:To assess whether PCR is applicable for monitoring the efficacy of antituberculous treatment, respiratory specimens obtained during treatment and follow-up from sputum smear-positive tuberculosis (TB) patients were examined. First, results of smear, culture, and PCR for Mycobacterium tuberculosis complex (MTB) and an ...

journal_title：Journal of clinical microbiology

authors： Thomsen VO,Kok-Jensen A,Buser M,Philippi-Schulz S,Burkardt HJ

更新日期：1999-11-01 00:00:00

abstract：:We investigated the antibody response in women with postpartum fever from whom Ureaplasma urealyticum had been isolated from the bloodstream. Acute- and convalescent-phase sera were tested for immunoglobulin G to the polypeptides of five serovars (1, 2, 3, 4, and 8), representing the two genomic clusters of U. urealyt...

journal_title：Journal of clinical microbiology

authors： Lee GY,Kenny GE

更新日期：1987-10-01 00:00:00

abstract：:Members of the genus Exophiala are often difficult to identify to the species level because of their variable morphological appearances. This paper describes the methods used to identify Exophiala mesophila and provides salient differential features for distinguishing other mesophilic members of the genus. ...

journal_title：Journal of clinical microbiology

authors： Porteous NB,Grooters AM,Redding SW,Thompson EH,Rinaldi MG,De Hoog GS,Sutton DA

更新日期：2003-08-01 00:00:00

abstract：:A 25-year-old female was admitted to our intensive care unit with septic shock and multiorgan failure caused by extended-spectrum beta-lactamase-producing Escherichia coli originating from the right renal pelvis. A 16-day course of treatment with meropenem reversed the septic condition, but the infection recurred ther...

journal_title：Journal of clinical microbiology

authors： Krueger WA,Kempf VA,Peiffer M,Nagele U,Unertl KE,Schroeder TH

更新日期：2008-02-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

journal_title：Journal of clinical microbiology

authors： Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

更新日期：2013-02-01 00:00:00

abstract：:Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

journal_title：Journal of clinical microbiology

authors： McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

更新日期：2001-01-01 00:00:00

abstract：:Two commercially available reagents, latex particles (Bactigen) and Staphylococcus aureus suspensions (Phadebact), were compared for the detection of the capsular polyribitol phosphate antigen of Haemophilus influenzae type b in 18 pediatric patients with proven infections due to H. influenzae type b. Whereas both tes...

journal_title：Journal of clinical microbiology

authors： Riera L

更新日期：1985-04-01 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flo...

journal_title：Journal of clinical microbiology

authors： Boutal H,Naas T,Devilliers K,Oueslati S,Dortet L,Bernabeu S,Simon S,Volland H

更新日期：2017-07-01 00:00:00