Method for determining bioburden of surgical gloves.

abstract：:The diagnostic value of RT-PCR of amniotic fluid (AF) for prenatal diagnosis of congenital rubella virus infection in 45 pregnant women with confirmed primary infection was assessed. Specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%. ...

journal_title：Journal of clinical microbiology

authors： Macé M,Cointe D,Six C,Levy-Bruhl D,Parent du Châtelet I,Ingrand D,Grangeot-Keros L

更新日期：2004-10-01 00:00:00

abstract：:Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...

journal_title：Journal of clinical microbiology

authors： Huys G,Cnockaert M,Nemec A,Swings J

更新日期：2005-10-01 00:00:00

abstract：:During 2012, we identified sampled dogs with elevated levels of antibodies (≥1:40) against A(H1N1)pdm09 virus by using a hemagglutination inhibition (HI) assay (seroprevalence, 24.7%) and a microneutralization (MN) assay (seroprevalence, 10.8%). These high seroprevalences of A(H1N1)pdm09 among dogs without clinical si...

journal_title：Journal of clinical microbiology

authors： Su S,Chen J,Jia K,Khan SU,He S,Fu X,Hong M,Sun L,Qi W,Gray GC,Li S

更新日期：2014-05-01 00:00:00

abstract：:A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted...

journal_title：Journal of clinical microbiology

authors： Mellencamp MW,Pfeiffer NE,Suiter BT,Harness JR,Beckenhauer WH

更新日期：1989-10-01 00:00:00

abstract：:The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...

journal_title：Journal of clinical microbiology

authors： Fillerova R,Gallo J,Radvansky M,Kraiczova V,Kudelka M,Kriegova E

更新日期：2017-09-01 00:00:00

abstract：:Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

更新日期：2008-01-01 00:00:00

abstract：:The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifyin...

journal_title：Journal of clinical microbiology

authors： Arribas JR,Clifford DB,Fichtenbaum CJ,Roberts RL,Powderly WG,Storch GA

更新日期：1995-06-01 00:00:00

abstract：:A Legionella-like organism (strain 798-PA-H, ATCC 35999) isolated from a lung aspirate was shown by DNA hybridization to belong to the species Legionella hackeliae. Growth, gas-liquid chromatographic, and physiologic characteristics were consistent with those of the type strain of L. hackeliae, Lansing 2 (ATCC 35250)....

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Thacker WL,Steigerwalt AG,Brenner DJ,Ampel NM,Wing EJ

更新日期：1985-10-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The ac...

journal_title：Journal of clinical microbiology

authors： Mickelsen PA,McCarthy LR,Propst MA

更新日期：1977-03-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...

journal_title：Journal of clinical microbiology

authors： Jayarao BM,Doré JJ Jr,Oliver SP

更新日期：1992-09-01 00:00:00

abstract：:The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI ...

journal_title：Journal of clinical microbiology

authors： Melter O,Aires de Sousa M,Urbásková P,Jakubů V,Zemlicková H,de Lencastre H

更新日期：2003-11-01 00:00:00

abstract：:High-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. Isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees C. After removal of organis...

journal_title：Journal of clinical microbiology

authors： Harpold DJ,Wasilauskas BL

更新日期：1987-06-01 00:00:00

abstract：:We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than...

journal_title：Journal of clinical microbiology

authors： Reller ME,Zaidi AK,Sultana S,Azeem S,Hanif B,Qureshi S,Hasan R,Bhutta Z,Akhter R,Goldmann DA

更新日期：2009-01-01 00:00:00

abstract：:Toxoplasmic lymphadenopathy (TL) is the most common clinical manifestation of acute acquired toxoplasma infection in normal individuals. The diagnosis is established by serologic methods and lymph node biopsy. Recently, tests for avidity of toxoplasma immunoglobulin G (IgG) antibodies have been introduced to help disc...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Huffman HB,Remington JS

更新日期：2004-10-01 00:00:00

abstract：:The L1 major capsid proteins of six human papillomavirus type 16 (HPV-16) strains were expressed in insect cells by using recombinant baculoviruses. Virus-like particles (VLPs) which appeared similar to empty virions were identified by electron microscopy for all HPV strains investigated. However, the yield of VLPs pr...

journal_title：Journal of clinical microbiology

authors： Touze A,El Mehdaoui S,Sizaret PY,Mougin C,Muñoz N,Coursaget P

更新日期：1998-07-01 00:00:00

abstract：:Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu-->Trp, 87-Asp-->Ala, and 106-Gln-->Arg) and three novel parC mutatio...

journal_title：Journal of clinical microbiology

authors： Dimitrov T,Dashti AA,Albaksami O,Udo EE,Jadaon MM,Albert MJ

更新日期：2009-01-01 00:00:00

abstract：:To objectively assess the value of examining multiple sputum specimens in maximizing the sensitivity of detection of Mycobacterium tuberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients diagnosed with culture-proven pulmonary tuberculosis (TB) at Hennepin County Medical ...

journal_title：Journal of clinical microbiology

authors： Nelson SM,Deike MA,Cartwright CP

更新日期：1998-02-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

journal_title：Journal of clinical microbiology

authors： Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

更新日期：2012-12-01 00:00:00

abstract：:An Escherichia coli rRNA probe was compared with a combination of O serotyping, phage susceptibility, and biotype pattern for the type identification of strains of Enterobacter cloacae. Forty-five isolates of E. cloacae from 36 patients in nine hospitals were examined. By conventional typing, only 26 (57.7%) could be ...

journal_title：Journal of clinical microbiology

authors： Garaizar J,Kaufmann ME,Pitt TL

更新日期：1991-07-01 00:00:00

abstract：:From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patie...

journal_title：Journal of clinical microbiology

authors： Grillová L,Pĕtrošová H,Mikalová L,Strnadel R,Dastychová E,Kuklová I,Kojanová M,Kreidlová M,Vaňousová D,Hercogová J,Procházka P,Zákoucká H,Krchňáková A,Vašků V,Šmajs D

更新日期：2014-10-01 00:00:00

abstract：:Statistical analysis of a limiting dilution assay (LDA) showed that the occurrence of infectious human immunodeficiency virus type 1 (HIV-1)-harboring cells in serially diluted samples of peripheral blood mononuclear cells (PBMCs) of HIV-1-seropositive patients fits the model describing a single-hit Poisson distributi...

journal_title：Journal of clinical microbiology

authors： Lu W,Manolikakis G,Andrieu JM

更新日期：1992-10-01 00:00:00

abstract：:Two rapid diagnostic tests for Chlamydia trachomatis (Microtrak, Syva Co., Palo Alto, Calif.; and Chlamydiazyme, Abbott Laboratories, North Chicago, Ill.) were evaluated in comparison with growth of the organism in tissue culture for 2,030 urogenital specimens from men and women. Neither test performed as well as cult...

journal_title：Journal of clinical microbiology

authors： Hipp SS,Han Y,Murphy D

更新日期：1987-10-01 00:00:00

abstract：:To investigate the interaction of Limulus amoebocyte lysate (LAL) with gram-negative bacteria, soluble antigens from sonicated Pseudomonas aeruginosa were studied by various crossed-immunoelectrophoresis methods before and after reaction with LAL. Of 64 possible, at least 7 antigens were affected, as indicated by prec...

journal_title：Journal of clinical microbiology

authors： Baek L,Høiby N,Hertz JB,Espersen F

更新日期：1985-08-01 00:00:00

abstract：:An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34 medical centers (22 in the United States, 6 in Canada, and 6 in South America). Eighty percent of the BSIs were nosocomia...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Jones RN,Doern GV,Sader HS,Hollis RJ,Messer SA

更新日期：1998-07-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:To determine sensitivity and specificity of immunofluorescence assay (IFA) for detection of Helicobacter pylori, we studied 151 patients. Biopsies of gastric mucosae were obtained for culture, histological testing, and IFA. H. pylori serum antibodies were tested by enzyme-linked immunosorbent assay. IFA was done on Fo...

journal_title：Journal of clinical microbiology

authors： Rivera E,López-Vidal Y,Luqueño V,Ruiz-Palacios GM

更新日期：1991-08-01 00:00:00