International surveillance of bloodstream infections due to Candida species: frequency of occurrence and antifungal susceptibilities of isolates collected in 1997 in the United States, Canada, and South America for the SENTRY Program. The SENTRY Participa


:An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34 medical centers (22 in the United States, 6 in Canada, and 6 in South America). Eighty percent of the BSIs were nosocomial and 50% occurred in patients hospitalized in an intensive care unit. Overall, 53.3% of the BSIs were due to Candida albicans, 15.7% were due to C. parapsilosis, 15.0% were due to C. glabrata, 7.8% were due to C. tropicalis, 2.0% were due to C. krusei, 0.7% were due to C. guilliermondii, and 5.8% were due to Candida spp. However, the distribution of species varied markedly by country. In the United States, 43.8% of BSIs were due to non-C. albicans species. C. glabrata was the most common non-C. albicans species in the United States. The proportion of non-C. albicans BSIs was slightly higher in Canada (47.5%), where C. parapsilosis, not C. glabrata, was the most common non-C. albicans species. C. albicans accounted for 40.5% of all BSIs in South America, followed by C. parapsilosis (38.1%) and C. tropicalis (11.9%). Only one BSI due to C. glabrata was observed in South American hospitals. Among the different species of Candida, resistance to fluconazole (MIC, > or = 64 microg/ml) and itraconazole (MIC, > or = 1.0 microg/ml) was observed with C. glabrata and C. krusei and was observed more rarely among other species. Isolates of C. albicans, C. parapsilosis, C. tropicalis, and C. guilliermondii were all highly susceptible to both fluconazole (99.4 to 100% susceptibility) and itraconazole (95.8 to 100% susceptibility). In contrast, 8.7% of C. glabrata isolates (MIC at which 90% of isolates are inhibited [MIC90], 32 microg/ml) and 100% of C. krusei isolates were resistant to fluconazole, and 36.9% of C. glabrata isolates (MIC90, 2.0 microg/ml) and 66.6% of C. krusei isolates were resistant to itraconazole. Within each species there were no geographic differences in susceptibility to fluconazole or itraconazole.


J Clin Microbiol


Pfaller MA,Jones RN,Doern GV,Sader HS,Hollis RJ,Messer SA




Has Abstract


1998-07-01 00:00:00












  • Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species.

    abstract::We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Murdoch DR,Mirrett S,Harrell LJ,Donabedian SM,Zervos MJ,Reller LB

    更新日期:2003-06-01 00:00:00

  • Genotypic and phenotypic characterization of Helicobacter cinaedi and Helicobacter fennelliae strains isolated from humans and animals.

    abstract::By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kiehlbauch JA,Brenner DJ,Cameron DN,Steigerwalt AG,Makowski JM,Baker CN,Patton CM,Wachsmuth IK

    更新日期:1995-11-01 00:00:00

  • Ability of new APTIMA CT and APTIMA GC assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in male urine and urethral swabs.

    abstract::A clinical evaluation was conducted in six North American centers to determine the ability of APTIMA CT (ACT) and APTIMA GC (AGC) nucleic acid amplification assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections in 1,322 men by testing their urethral swabs and first-catch urine (FCU). The results ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Chernesky MA,Martin DH,Hook EW,Willis D,Jordan J,Wang S,Lane JR,Fuller D,Schachter J

    更新日期:2005-01-01 00:00:00

  • Heated versus unheated sera in the hemagglutination treponemal test for syphilis.

    abstract::Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

    更新日期:1982-02-01 00:00:00

  • Comparative Clinical Evaluation of NeoPlex RB-8 with Seeplex PneumoBacter ACE for Simultaneous Detection of Eight Respiratory Bacterial Pathogens.

    abstract::There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

    更新日期:2020-01-28 00:00:00

  • Detection of transcripts of human papillomaviruses 16 and 18 in cancer-derived cell lines and cervical biopsies by enzyme immunoassay for DNA-RNA hybrids following solution hybridization.

    abstract::mRNAs of human papillomaviruses (HPV) 16 and 18 were detected in cancer-derived cell lines and genital tract biopsy specimens by a novel hybridization assay. Biotinylated whole genomic HPV DNA probes were hybridized in solution to extracted total nucleic acids. Hybrids between the labeled probes and RNA transcripts we...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Coutlée F,Shah KV,Rader JS,Currie JL,Viscidi RP

    更新日期:1991-05-01 00:00:00

  • Development of poliovirus extraction method from stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor.

    abstract::A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude bi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Arita M

    更新日期:2013-08-01 00:00:00

  • Identification of Mycobacterium tuberculosis and M. avium complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models.

    abstract::A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

    更新日期:1995-05-01 00:00:00

  • Use of the API NeIdent system for identification of pathogenic Neisseria spp. and Branhamella catarrhalis.

    abstract::The API NeIdent system (Analytab Products, Plainview, N.Y.) was evaluated for identifying Neisseria spp. and Branhamella catarrhalis commonly isolated from clinical specimens. The system identified 90% of 303 Neisseria gonorrhoeae isolates, 71% of 113 Neisseria meningitidis isolates, and 63% of 16 Neisseria lactamica ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Janda WM,Morello JA,Bohnhoff M

    更新日期:1984-03-01 00:00:00

  • Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium.

    abstract::A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Restrepo A,Jiménez BE

    更新日期:1980-08-01 00:00:00

  • Convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.

    abstract::A simple disk diffusion test was constructed for detection of IMP-1-type metallo-beta-lactamase-producing gram-negative bacteria. Two Kirby-Bauer disks containing ceftazidime (CAZ) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. Several IMP-1 inhibitors such as thiol compounds i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Arakawa Y,Shibata N,Shibayama K,Kurokawa H,Yagi T,Fujiwara H,Goto M

    更新日期:2000-01-01 00:00:00

  • Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

    abstract::We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suita...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hrncirova K,Lengerova M,Kocmanova I,Racil Z,Volfova P,Palousova D,Moulis M,Weinbergerova B,Winterova J,Toskova M,Pospisilova S,Mayer J

    更新日期:2010-09-01 00:00:00

  • Characterization of Streptococcus agalactiae strains by randomly amplified polymorphic DNA analysis.

    abstract::A collection of 54 unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid samples from neonates and 60 unrelated strains isolated from carriers that had been previously studied by multilocus enzyme electrophoresis (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Cli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chatellier S,Ramanantsoa C,Harriau P,Rolland K,Rosenau A,Quentin R

    更新日期:1997-10-01 00:00:00

  • Septic arthritis involving Capnocytophaga ochracea.

    abstract::Septic arthritis of the knee developed in a 21-month-old child. The causative organism, isolated from two separate arthrocenteses, was identified as Capnocytophaga ochracea morphologically and by biochemical reactions. Previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant ora...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Winn RE,Chase WF,Lauderdale PW,McCleskey FK

    更新日期:1984-04-01 00:00:00

  • Comparison between viremia and antigenemia for detection of cytomegalovirus in blood.

    abstract::In a prospective study, 139 serial blood samples from 15 transplant recipients were assessed for the presence of cytomegalovirus (CMV) by virus isolation (CMV viremia) and by direct staining of CMV antigens (CMV Ag) in blood leukocytes (CMV antigenemia). CMV was isolated from 23 samples, whereas CMV Ag was detected in...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: van der Bij W,Schirm J,Torensma R,van Son WJ,Tegzess AM,The TH

    更新日期:1988-12-01 00:00:00

  • Detection of Mycobacterium tuberculosis by EasyNAT diagnostic kit in sputum samples from Tanzania.

    abstract::The EasyNAT assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (TB) patients in an African high-TB and high-HIV setting. The sensitivity of the EasyNAT assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patie...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mhimbira FA,Bholla M,Sasamalo M,Mukurasi W,Hella JJ,Jugheli L,Reither K

    更新日期:2015-04-01 00:00:00

  • Comparative evaluation of the API 20S and AutoMicrobic gram-positive identification systems for non-beta-hemolytic streptococci and aerococci.

    abstract::The API 20S system (Analytab Products, Plainview, N.Y.) and the AutoMicrobic Gram-Positive Identification system (GPI; Vitek Systems, Hazelwood, Mo.) were evaluated for their capacity to identify the non-beta-hemolytic streptococci and aerococci to the species level. The 20S system identified 86% (six of seven strains...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Facklam R,Bosley GS,Rhoden D,Franklin AR,Weaver N,Schulman R

    更新日期:1985-04-01 00:00:00

  • Detection of Helicobacter pylori in gastric mucosa of patients with gastroduodenal diseases by PCR-restriction analysis using the RNA polymerase gene (rpoB).

    abstract::A novel PCR restriction analysis method using the RNA polymerase beta-subunit- coding gene (rpoB) was employed to both detect and identify Helicobacter pylori in biopsy specimens and culture isolates. The rpoB DNAs (458 bp) were specifically amplified by PCR with the Helicobacter-specific primers (HF and HR). Based on...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lim CY,Lee KH,Cho MJ,Chang MW,Kim SY,Myong NH,Lee WK,Rhee KH,Kook YH

    更新日期:2003-07-01 00:00:00

  • Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae.

    abstract::We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

    更新日期:2002-02-01 00:00:00

  • Comparison of commercially available group B streptococcal latex agglutination assays.

    abstract::Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ascher DP,Wilson S,Fischer GW

    更新日期:1991-12-01 00:00:00

  • Hepatitis C virus genotypes in northern Italy: clinical and virological features.

    abstract::We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

    更新日期:1996-11-01 00:00:00

  • Comparison of restriction endonuclease analysis, ribotyping, and pulsed-field gel electrophoresis for molecular differentiation of Clostridium difficile strains.

    abstract::A combined clinical and molecular epidemiologic analysis of 46 strains of Clostridium difficile, including 16 nosocomial isolates from one ward (outbreak ward) plus 17 other nosocomial isolates and 13 community-acquired isolates, was performed. HindIII digests of total cellular DNA were analyzed by restriction enzyme ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kristjánsson M,Samore MH,Gerding DN,DeGirolami PC,Bettin KM,Karchmer AW,Arbeit RD

    更新日期:1994-08-01 00:00:00

  • Clonal structure of Enterococcus faecalis isolated from Polish hospitals: characterization of epidemic clones.

    abstract::To study the population structure of Enterococcus faecalis from Polish hospitals, 291 isolates were typed by pulsed-field gel electrophoresis and a novel multilocus sequence typing scheme (P. Ruiz-Garbajosa et al., J. Clin. Microbiol. 44:2220-2228, 2006). The isolates originated from geographically widespread medical ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kawalec M,Pietras Z,Daniłowicz E,Jakubczak A,Gniadkowski M,Hryniewicz W,Willems RJ

    更新日期:2007-01-01 00:00:00

  • Sensitive detection of Ehrlichia chaffeensis in cell culture, blood, and tick specimens by reverse transcription-PCR.

    abstract::Ehrlichia chaffeensis is an obligatory intracellular bacterium of monocytes and macrophages and the etiologic agent of human monocytic ehrlichiosis, an emerging zoonosis. The Lone Star tick (Amblyomma americanum) has been implicated as the primary vector of E. chaffeensis. The present study examined the sensitivity of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Felek S,Unver A,Stich RW,Rikihisa Y

    更新日期:2001-02-01 00:00:00

  • Toward molecular parasitologic diagnosis: enhanced diagnostic sensitivity for filarial infections in mobile populations.

    abstract::The diagnosis of filarial infections among individuals residing in areas where the disease is not endemic requires both strong clinical suspicion and expert training in infrequently practiced parasitological methods. Recently developed filarial molecular diagnostic assays are highly sensitive and specific but have lim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fink DL,Fahle GA,Fischer S,Fedorko DF,Nutman TB

    更新日期:2011-01-01 00:00:00

  • Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes.

    abstract::Giardia spp. are waterborne organisms that are the most commonly identified pathogenic intestinal protozoans in the United States. Current detection techniques for Giardia species in water include microscopy and immunofluorescence techniques. Species of the genus Giardia are classified on the basis of taxonomic criter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mahbubani MH,Bej AK,Perlin MH,Schaefer FW 3rd,Jakubowski W,Atlas RM

    更新日期:1992-01-01 00:00:00

  • Sequencing of the pncA gene in members of the Mycobacterium tuberculosis complex has important diagnostic applications: Identification of a species-specific pncA mutation in "Mycobacterium canettii" and the reliable and rapid predictor of pyrazinamide res

    abstract::Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

    更新日期:2007-02-01 00:00:00

  • Disseminated rhinovirus C8 infection with infectious virus in blood and fatal outcome in a child with repeated episodes of bronchiolitis.

    abstract::We report a fatal case of acute lower respiratory tract disease with human rhinovirus C (HRV-C) as the unique cause in a 19-month-old girl with a history of repeated episodes of bronchiolitis. HRV-C type 8 nucleic acids were observed in respiratory, stool, and cerebrospinal fluid samples, and infectious virions were i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lupo J,Schuffenecker I,Morel-Baccard C,Bardet J,Payen V,Kaiser L,Constant S,Lobrinus JA,Lin-Marq N,Lina B,Morand P,Tapparel C

    更新日期:2015-05-01 00:00:00

  • Unique p24 epitope marker to identify multiple human immunodeficiency virus variants in blood from the same individuals.

    abstract::The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Bolton V,Pedersen NC,Higgins J,Jennings M,Carlson J

    更新日期:1987-08-01 00:00:00

  • Comparison of Manual and Fully Automated AIX1000 Rapid Plasma Reagin Assays for Laboratory Diagnosis of Syphilis.

    abstract::The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sanfilippo AM,Freeman K,Schmitz JL

    更新日期:2018-07-26 00:00:00