Correlation between biochemical and serological characteristics of Escherichia coli and results of the Serény test.

abstract：:We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding ...

journal_title：Journal of clinical microbiology

authors： Collantes-Fernández E,Zaballos A,Alvarez-García G,Ortega-Mora LM

更新日期：2002-04-01 00:00:00

abstract：:During 5.5 years of a study in Washington, D.C., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. Overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteri...

journal_title：Journal of clinical microbiology

authors： Brandt CD,Kim HW,Rodriguez WJ,Arrobio JO,Jeffries BC,Parrott RH

更新日期：1982-09-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:A monoclonal antibody specific for the gI glycoprotein of virulent pseudorabies virus was produced and used to affinity purify gI glycoprotein. The purified gI was used in an enzyme-linked immunosorbent assay (ELISA) that identified and differentiated field virus-exposed animals from animals vaccinated with gI-deleted...

journal_title：Journal of clinical microbiology

authors： Mellencamp MW,Pfeiffer NE,Suiter BT,Harness JR,Beckenhauer WH

更新日期：1989-10-01 00:00:00

abstract：:Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...

journal_title：Journal of clinical microbiology

authors： Betrán A,Rezusta A,Lezcano MA,Villuendas MC,Revillo MJ,Boiron P,Rodríguez-Nava V

更新日期：2009-06-01 00:00:00

abstract：:We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K. Bohachick, and C.M. Patton, J. Clin. Microbiol. 31:1531-1536, 1993). We extended the typing system to 83 flagellin types...

journal_title：Journal of clinical microbiology

authors： Nachamkin I,Ung H,Patton CM

更新日期：1996-02-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:The ability of antibody induced by vaccination with recombinant gp160 (rgp160) to bind to native and recombinant human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins was measured. Thirty-three HIV-1-seronegative healthy adult volunteers were injected four times with 40 or 80 micrograms of an HIV-1LAV env...

journal_title：Journal of clinical microbiology

authors： Gorse GJ,Frey SE,Newman FK,Belshe RB

更新日期：1992-10-01 00:00:00

abstract：:Several instruments for automated or semiautomated susceptibility testing are currently available. Three of these instruments, Autobac (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.), MS-2 (Abbott Laboratories, Dallas, Tex.), and AutoMicrobic system (AMS) (Vitek, Inc., Hazelwood, Mo.) were compared for ...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Latimer JM,Balfour LC

更新日期：1982-05-01 00:00:00

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:Real-time PCR analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. This suggests that an unresolved complexity exists in bacteria associated with advanced caries. In this report, the profile of the microflora of carious dentine was ex...

journal_title：Journal of clinical microbiology

authors： Chhour KL,Nadkarni MA,Byun R,Martin FE,Jacques NA,Hunter N

更新日期：2005-02-01 00:00:00

abstract：:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...

journal_title：Journal of clinical microbiology

authors： Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MH

更新日期：2002-08-01 00:00:00

abstract：:The EasyNAT assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (TB) patients in an African high-TB and high-HIV setting. The sensitivity of the EasyNAT assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patie...

journal_title：Journal of clinical microbiology

authors： Mhimbira FA,Bholla M,Sasamalo M,Mukurasi W,Hella JJ,Jugheli L,Reither K

更新日期：2015-04-01 00:00:00

abstract：:In a group of 596 delivering Ghanaian women, the sensitivities of peripheral blood thick film microscopy, ICT Malaria P.f/P.v test, and PCR in detecting microscopically confirmed placental Plasmodium falciparum infection were 42, 80, and 97%, respectively. In addition to the gross underestimation of placental malaria ...

journal_title：Journal of clinical microbiology

authors： Mockenhaupt FP,Ulmen U,von Gaertner C,Bedu-Addo G,Bienzle U

更新日期：2002-01-01 00:00:00

abstract：:Fusarium species are saprophytic molds which cause disseminated or localized infections in humans. Disseminated Fusarium infection can cause significant morbidity and mortality in immunocompromised patients. We present a case of disseminated fusariosis caused by Fusarium verticillioides in a patient with acute lymphob...

journal_title：Journal of clinical microbiology

authors： Tezcan G,Ozhak-Baysan B,Alastruey-Izquierdo A,Ogunc D,Ongut G,Yildiran ST,Hazar V,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2009-01-01 00:00:00

abstract：:Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

journal_title：Journal of clinical microbiology

authors： Woods GL,Witebsky FG

更新日期：1995-02-01 00:00:00

abstract：:A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

journal_title：Journal of clinical microbiology

authors： Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

更新日期：1989-05-01 00:00:00

abstract：:Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...

journal_title：Journal of clinical microbiology

authors： McIver CJ,Jacques CF,Chow SS,Munro SC,Scott GM,Roberts JA,Craig ME,Rawlinson WD

更新日期：2005-10-01 00:00:00

abstract：:We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favor...

journal_title：Journal of clinical microbiology

authors： Meyers PR,Bourn WR,Steyn LM,van Helden PD,Beyers AD,Brown GD

更新日期：1998-09-01 00:00:00

abstract：:Ninety-eight isolates of Cryptococcus neoformans were collected from 30 patients at the University of Iowa Hospitals and Clinics from December 1987 through December 1994. The susceptibility of each isolate was determined against fluconazole, itraconazole, amphotericin B, and flucytosine. Of the 98 isolates, 53 were re...

journal_title：Journal of clinical microbiology

authors： Klepser ME,Pfaller MA

更新日期：1998-12-01 00:00:00

abstract：:Osiris is a video zone size reader for disk diffusion tests featuring a built-in extended expert system (EES). The efficacy of the EES for the identification of the beta-lactam susceptibility phenotypes of Pseudomonas aeruginosa isolates was evaluated. Thirteen beta-lactams were tested in four laboratories by the disk...

journal_title：Journal of clinical microbiology

authors： Bert F,Ould-Hocine Z,Juvin M,Dubois V,Loncle-Provot V,Lefranc V,Quentin C,Lambert N,Arlet G

更新日期：2003-08-01 00:00:00

abstract：:Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

journal_title：Journal of clinical microbiology

authors： Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

更新日期：2009-04-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00

abstract：:By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cin...

journal_title：Journal of clinical microbiology

authors： Kiehlbauch JA,Brenner DJ,Cameron DN,Steigerwalt AG,Makowski JM,Baker CN,Patton CM,Wachsmuth IK

更新日期：1995-11-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:We serotyped 222 Pasteurella multocida strains isolated from swine lungs at slaughter. Capsular serotypes A and D were determined by the hyaluronidase sensitivity and acriflavin agglutination tests, respectively. Somatic antigens were determined by gel diffusion against standard antisera. Capsular serotype A was found...

journal_title：Journal of clinical microbiology

authors： Pijoan C,Morrison RB,Hilley HD

更新日期：1983-06-01 00:00:00

abstract：:A total of 242 isolates were recovered from 76 patients with invasive diseases, 89 with scarlet fever, and 77 with pharyngitis. The most frequent emm types were types 12 (43.4%), 4 (18.2%), and 1 (16.9%). emm12 reemerged in 2005 and peaked in 2007. emm11 was recovered only from patients with invasive disease. ...

journal_title：Journal of clinical microbiology

authors： Su YF,Wang SM,Lin YL,Chuang WJ,Lin YS,Wu JJ,Lin MT,Liu CC

更新日期：2009-08-01 00:00:00

abstract：:This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

更新日期：2002-10-01 00:00:00

abstract：:A modified indirect hemagglutination test for the detection of treponemal antibodies was developed for use with finger-prick blood. By using paired serum and absorbed finger-prick blood from 58 patients from an area previously endemic for yaws and 12 patients without yaws, the modified hemagglutination test was compar...

journal_title：Journal of clinical microbiology

authors： Backhouse JL,Lee MH,Nesteroff SI,Hudson BJ,Hamilton PA

更新日期：1992-03-01 00:00:00

abstract：:The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...

journal_title：Journal of clinical microbiology

authors： Wells CL,Field CR

更新日期：1976-12-01 00:00:00