Abstract:
:Polyclonal anti-idiotypic antibodies (anti-Ids) were generated by the sequential immunization of rabbits with three mouse monoclonal antibodies (MAb1s) specific for a major bluetongue virus (BTV) protein, VP7. The anti-Ids, designated RAb2s, recognized idiotopes which were located within or near the antigen-combining sites of the MAb1s and were associated with both heavy and light chains of MAb1s. RAb2s inhibited the MAb1s from binding to BTV antigens, and their interaction with MAb1s was inhibited by BTV antigens. By recognizing the common idiotopes, RAb2s detected anti-BTV antibodies from bovine antisera; their interaction was also partially inhibited by BTV antigens. These results indicated that RAb2s recognized the common idiotopes on anti-VP7 antibodies obtained from mice and cattle and that at least a portion of RAb2s were internal-image anti-Ids that functionally mimicked VP7. RAb2s may be used to substitute for the tissue culture-derived viral antigen in currently used serological assays for the detection of antibodies to BTV.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Zhou EM,Huang Wdoi
10.1128/JCM.33.4.850-854.1995subject
Has Abstractpub_date
1995-04-01 00:00:00pages
850-4issue
4eissn
0095-1137issn
1098-660Xjournal_volume
33pub_type
杂志文章abstract::A LightCycler-based PCR protocol was developed which targets the ospA gene for the identification and quantification of the different Borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (HybProbe) and an internally labeled primer. The detection limit of th...
journal_title:Journal of clinical microbiology
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doi:10.1128/jcm.40.1.36-43.2002
更新日期:2002-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.38.2.702-707.2000
更新日期:2000-02-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.01977-14
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.6.1049-1052.1984
更新日期:1984-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.3241-3245.2003
更新日期:2003-07-01 00:00:00
abstract::The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.5.850-854.1981
更新日期:1981-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.10.3718-3720.2001
更新日期:2001-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.44.2.468-473.2006
更新日期:2006-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.21.3.332-334.1985
更新日期:1985-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.5.1232-1236.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.6.1579-1585.1993
更新日期:1993-06-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.2.589-595.2005
更新日期:2005-02-01 00:00:00
abstract::We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.5.1141-1145.1983
更新日期:1983-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.12.1.88-94.1980
更新日期:1980-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01147-07
更新日期:2007-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.3.1448-1450.2005
更新日期:2005-03-01 00:00:00
abstract::Enterobacter cloacae strain G6809 with reduced susceptibility to carbapenems was identified from a patient in a long-term acute care hospital in Kentucky. G6809 belonged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1). Whole-genome sequencing localized bla(KPC-18) to the chromo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02739-15
更新日期:2016-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.10.3370-3373.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 历史文章,杂志文章,评审
doi:10.1128/JCM.03394-15
更新日期:2016-05-01 00:00:00
abstract::A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00650-11
更新日期:2011-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.5.835-837.1985
更新日期:1985-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00904-13
更新日期:2013-08-01 00:00:00
abstract::At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds had completely healed, without complicated infections, when the cellu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01507-06
更新日期:2007-01-01 00:00:00
abstract::Metronidazole and tetracycline E tests were compared to an agar dilution method for the antimicrobial susceptibility testing of Helicobacter pylori. Sixteen strains were tested by using tetrazolium egg yolk (TEY) agar. The characteristic E test inhibition ellipse was clearer on TEY agar than on standard blood agar and...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.9.2784-2785.1998
更新日期:1998-09-01 00:00:00