Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis.

abstract：:Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated...

journal_title：Journal of clinical microbiology

authors： Trip H,Mende K,Majchrzykiewicz-Koehorst JA,Sedee NJ,Hulst AG,Jansen HJ,Murray CK,Paauw A

更新日期：2015-06-01 00:00:00

abstract：:A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire...

journal_title：Journal of clinical microbiology

authors： Chen JHK,Lam HY,Yip CCY,Wong SCY,Chan JFW,Ma ESK,Cheng VCC,Tang BSF,Yuen KY

更新日期：2016-07-01 00:00:00

abstract：:A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific for the region of the RSV genome which enco...

journal_title：Journal of clinical microbiology

authors： Paton AW,Paton JC,Lawrence AJ,Goldwater PN,Harris RJ

更新日期：1992-04-01 00:00:00

abstract：:Duck hepatitis B virus (DHBV) belongs to the Hepadnaviridae family, which includes human Hepatitis B virus (HBV) and Woodchuck hepatitis virus. It is widely distributed in wild and domestic ducks due to congenital transmission. HBV is a worldwide health problem, with carriers at risk of developing cirrhosis and liver ...

journal_title：Journal of clinical microbiology

authors： Wang CY,Giambrone JJ,Smith BF

更新日期：2002-07-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00

abstract：:Four hundred thirty-two lactose-negative colonies isolated from human feces on stool differential agar media were flooded with one drop of MUCAP Test reagent (Biolife Italiana S.r.I., Milan, Italy) and observed under a Wood lamp for the development of a blue fluorescence over or around the colony. On the basis of manu...

journal_title：Journal of clinical microbiology

authors： Aguirre PM,Cacho JB,Folgueira L,López M,García J,Velasco AC

更新日期：1990-01-01 00:00:00

abstract：:Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

journal_title：Journal of clinical microbiology

authors： Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

更新日期：1996-05-01 00:00:00

abstract：:A pseudooutbreak of cryptococcal meningitis was caused by the use of contaminated albumin solution used in the preparation of Papanicolaou-stained slides of cerebrospinal fluid. Organisms were seen in cytocentrifuge preparations, but not in India ink preparations of cerebrospinal fluid specimens. Cryptococcal antigen ...

journal_title：Journal of clinical microbiology

authors： Hopfer RL,Katz RL,Fainstein V

更新日期：1982-06-01 00:00:00

abstract：:Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

journal_title：Journal of clinical microbiology

authors： Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

更新日期：2000-04-01 00:00:00

abstract：:A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of...

journal_title：Journal of clinical microbiology

authors： Jones LM,Berman DT,Moreno E,Deyoe BL,Gilsdorf MJ,Huber JD,Nicoletti P

更新日期：1980-12-01 00:00:00

abstract：:Candidate live-virus vaccines for respiratory syncytial virus are being developed and are beginning to be evaluated in clinical trials. To distinguish candidate vaccine strains from wild-type strains isolated during these trials, we developed PCR assays specific to two sets of candidate vaccine strains. The two sets w...

journal_title：Journal of clinical microbiology

authors： Zheng H,Peret TC,Randolph VB,Crowley JC,Anderson LJ

更新日期：1996-02-01 00:00:00

abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

journal_title：Journal of clinical microbiology

authors： Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

更新日期：2002-11-01 00:00:00

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

journal_title：Journal of clinical microbiology

authors： Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

更新日期：2007-06-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:The pathogenesis of human rhinovirus (HRV) during severe respiratory disease remains undefined; thus, we aimed to explore the relationship between the HRV molecular subtyping results obtained during severe and asymptomatic childhood infections. Nasopharyngeal/oropharyngeal swabs from children (1 to 59 months of age) h...

journal_title：Journal of clinical microbiology

authors： Baillie VL,Moore DP,Mathunjwa A,Morailane P,Simões EAF,Madhi SA

更新日期：2019-08-26 00:00:00

abstract：:Bartonella henselae is the main causative agent of cat-scratch disease, and both B. henselae and Bartonella quintana cause angioproliferative disorders such as bacillary angiomatosis. To increase the sensitivity of Bartonella detection by PCR and to improve the species differentiation, we developed a semiquantitative,...

journal_title：Journal of clinical microbiology

authors： Sander A,Penno S

更新日期：1999-10-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We comp...

journal_title：Journal of clinical microbiology

authors： Ferreira L,Sánchez-Juanes F,González-Avila M,Cembrero-Fuciños D,Herrero-Hernández A,González-Buitrago JM,Muñoz-Bellido JL

更新日期：2010-06-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:This is the first reported case of native valve endocarditis caused by Engyodontium album. This fungus, rarely seen as a human pathogen, is separated from Tritirachium species by its lack of pigmentation and from Beauveria species by the presence of conidiogenous cells in whorls. ...

journal_title：Journal of clinical microbiology

authors： Augustinsky J,Kammeyer P,Husain A,deHoog GS,Libertin CR

更新日期：1990-06-01 00:00:00

abstract：:The increasing number of multidrug-resistant Mycobacterium tuberculosis strains has stimulated the interest of investigators in finding a rapid method for susceptibility testing. We used commercially available rRNA DNA-bioluminescence-labelled probes (Accu-Probe, Gen Probe, Inc. San Diego, Calif.) for this purpose. Th...

journal_title：Journal of clinical microbiology

authors： Martin-Casabona N,Xairó Mimó D,González T,Rossello J,Arcalis L

更新日期：1997-10-01 00:00:00

abstract：:The taxonomic relationship of Rhinosporidium seeberi with other organisms remained controversial for over a century. Recently, molecular studies have shown R. seeberi to be a protistal microbe in the newly described class Mesomycetozoea at the animal-fungal boundary. Phylogenetic analyses of R. seeberi using 18S small...

journal_title：Journal of clinical microbiology

authors： Silva V,Pereira CN,Ajello L,Mendoza L

更新日期：2005-04-01 00:00:00

abstract：:The etiologies of a number of retinopathies, including serpiginous choroiditis and acute zonal occult outer retinopathy (AZOOR), remain uncertain. Recently, we provided evidence that AZOOR is caused by Candida famata infection. The purpose of this article was to investigate the presence of fungal infection in five pat...

journal_title：Journal of clinical microbiology

authors： Pisa D,Ramos M,García P,Escoto R,Barraquer R,Molina S,Carrasco L

更新日期：2008-01-01 00:00:00

abstract：:A dot immunobinding assay that uses inactivated antigen for the detection of rabies viral antibodies was compared with the rapid fluorescent focus inhibition test. Results of testing pre- and postvaccination sera from humans (n = 33) and canines (n = 22) were identical for both tests. Endpoint titers of positive sera ...

journal_title：Journal of clinical microbiology

authors： Heberling RL,Kalter SS,Smith JS,Hildebrand DG

更新日期：1987-07-01 00:00:00

abstract：:The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...

journal_title：Journal of clinical microbiology

authors： Haque R,Kabir M,Noor Z,Rahman SM,Mondal D,Alam F,Rahman I,Al Mahmood A,Ahmed N,Petri WA Jr

更新日期：2010-08-01 00:00:00

abstract：:Antiserum specific for Salmonella O3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-D- Manp (1----4)-alpha-L- Rhap (1----3)-alpha-D-Galp covalently linked to bovine serum albumin (beta- MRG -BSA). Enzyme immunoassays showed that only lipop...

journal_title：Journal of clinical microbiology

authors： Ekwall E,Norberg T,Svenson SB,Lindberg AA

更新日期：1984-05-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00

abstract：:The nasopharyngeal Haemophilus influenzae flora of healthy children in a day care center was analyzed by repeated sampling during 4 winter months. The average carrier rate was 39%, but 74% of the children became colonized at some time during the study. The H. influenzae isolates were identified by capsular type, bioty...

journal_title：Journal of clinical microbiology

authors： Trottier S,Stenberg K,Svanborg-Edén C

更新日期：1989-10-01 00:00:00

abstract：:While the majority of avian influenza virus (AIV) subtypes are classified as low-pathogenicity avian influenza viruses (LPAIV), the H5 and H7 subtypes have the ability to mutate to highly pathogenic avian influenza viruses (HPAIV) in poultry and therefore are the etiological agents of notifiable AIV (NAIV). It is of g...

journal_title：Journal of clinical microbiology

authors： Leijon M,Ullman K,Thyselius S,Zohari S,Pedersen JC,Hanna A,Mahmood S,Banks J,Slomka MJ,Belák S

更新日期：2011-11-01 00:00:00