Turnover of nontypable Haemophilus influenzae in the nasopharynges of healthy children.

abstract：:The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with r...

journal_title：Journal of clinical microbiology

authors： Fenollar F,Fournier PE,Robert C,Raoult D

更新日期：2004-01-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00

abstract：:Two molecular fingerprinting techniques, pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP), were used to investigate the epidemiological relatedness among Clostridium difficile isolates from suspected outbreaks in three general hospitals. Analysis by PFGE yielded inconclusive da...

journal_title：Journal of clinical microbiology

authors： Klaassen CH,van Haren HA,Horrevorts AM

更新日期：2002-01-01 00:00:00

abstract：:Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A s...

journal_title：Journal of clinical microbiology

authors： Sullivan MJ,Altman DR,Chacko KI,Ciferri B,Webster E,Pak TR,Deikus G,Lewis-Sandari M,Khan Z,Beckford C,Rendo A,Samaroo F,Sebra R,Karam-Howlin R,Dingle T,Hamula C,Bashir A,Schadt E,Patel G,Wallach F,Kasarskis A,Gi

更新日期：2019-11-22 00:00:00

abstract：:A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to...

journal_title：Journal of clinical microbiology

authors： Turba ME,Zambon E,Zannoni A,Russo S,Gentilini F

更新日期：2012-08-01 00:00:00

abstract：:Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrap...

journal_title：Journal of clinical microbiology

authors： Ramírez JR,Agudelo S,Muskus C,Alzate JF,Berberich C,Barker D,Velez ID

更新日期：2000-10-01 00:00:00

abstract：:A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...

journal_title：Journal of clinical microbiology

authors： Mankikar SD,Middleton PJ,Petric M

更新日期：1979-08-01 00:00:00

abstract：:Of 382 strains tested, 261 strains of the species Bacteroides fragilis, B. thetaiotaomicron, B. ovatus, and B. distasonis produced phenylacetic acid; the remaining strains, belonging exclusively to the species B. vulgatus, failed to do so. This differentiation characteristic may be useful in routine clinical bacteriol...

journal_title：Journal of clinical microbiology

authors： van Assche PF

更新日期：1978-11-01 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, ...

journal_title：Journal of clinical microbiology

authors： Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

更新日期：1985-11-01 00:00:00

abstract：:We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the ne...

journal_title：Journal of clinical microbiology

authors： Peter-Getzlaff S,Lüthy J,Voit A,Bloemberg GV,Böttger EC

更新日期：2010-11-01 00:00:00

abstract：:In a field trial conducted in cooperation with pediatricians in private practice, 198 nasopharyngeal swabs from children with suspected whooping cough were placed into charcoal-horse-blood transport medium with cephalexin (40 mg/liter). After preincubation at 36 degrees C for 1 to 2 days, the transport systems were ma...

journal_title：Journal of clinical microbiology

authors： Hoppe JE,Weiss A,Wörz S

更新日期：1988-06-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:Group A streptococci and enterococci were identified by two L-pyrrolidonyl-beta-naphthylamide (PYR) hydrolysis tests. One uses Minitek PYR disks (BBL Microbiology Systems, Cockeysville, Md.); the other uses a swab impregnated with PYR reagent. Both tests and Strep-A-Chek (EY Laboratories, San Mateo, Calif.), a commerc...

journal_title：Journal of clinical microbiology

authors： Wellstood SA

更新日期：1987-09-01 00:00:00

abstract：:The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Bernard CN

更新日期：2000-09-01 00:00:00

abstract：:The incidence of enterovirus 71 (EV71) infection has greatly increased in the Asian Pacific region since 1997. Several large outbreaks, caused by different subgenogroups of EV71, occurred with high rates of morbidity and a substantial number of deaths. In 2007, 58 cases of EV71 infection requiring hospitalization were...

journal_title：Journal of clinical microbiology

authors： van der Sanden S,Koopmans M,Uslu G,van der Avoort H,Dutch Working Group for Clinical Virology.

更新日期：2009-09-01 00:00:00

abstract：:Longitudinal data on the E6/E7 mRNA-based Aptima human papillomavirus (AHPV) assay exceeding three years in comparison to the gold standard Digene Hybrid Capture 2 (HC2) test are not available. We previously reported the cross-sectional data of the German AHPV Screening Trial (GAST) in which 10,040 women were recruite...

journal_title：Journal of clinical microbiology

authors： Iftner T,Neis KJ,Castanon A,Landy R,Holz B,Woll-Herrmann A,Iftner A,Staebler A,Wallwiener D,Hann von Weyhern C,Neis F,Haedicke-Jarboui J,Martus P,Brucker S,Henes M,Sasieni P

更新日期：2019-01-02 00:00:00

abstract：:The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with s...

journal_title：Journal of clinical microbiology

authors： Edner N,Castillo-Rodas P,Falk L,Hedman K,Söderlund-Venermo M,Allander T

更新日期：2012-02-01 00:00:00

abstract：:A technique is described which may be used to identify Haemophilus influenzae type b, Streptococcus pneumoniae, group A and B streptococci, and Staphylococcus aureus in smears of clinical specimens. ...

journal_title：Journal of clinical microbiology

authors： Clausen CR

更新日期：1981-06-01 00:00:00

abstract：:The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Pandey A,Tyrrell GJ

更新日期：2017-09-01 00:00:00

abstract：:The recent emergence of carbapenemase-producing Enterobacteriaceae strains represents a major threat for hospitalized patients. We document the dissemination and control of carbapenemase-producing Klebsiella pneumoniae clones in a Greek hospital. During a 3-year study period (January 2009 to December 2011), carbapenem...

journal_title：Journal of clinical microbiology

authors： Poulou A,Voulgari E,Vrioni G,Xidopoulos G,Pliagkos A,Chatzipantazi V,Markou F,Tsakris A

更新日期：2012-08-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:Triplicate preserved fecal samples from 265 patients were pooled into single samples, and the recovery rate of intestinal parasites from the pooled samples was compared with that from the individual samples. Parasites were detected in 109 patients when results from the individual samples were used compared with 108 pa...

journal_title：Journal of clinical microbiology

authors： Aldeen WE,Shisenant J,Hale D,Matsen J,Carroll K

更新日期：1993-01-01 00:00:00

abstract：:Early detection of Mycobacterium tuberculosis complex (MTBC) and markers conveying drug resistance can have a beneficial impact on preventive public health actions. We describe here a new molecular point-of-care (POC) system, the Genedrive, which is based on simple sample preparation combined with PCR to detect MTBC a...

journal_title：Journal of clinical microbiology

authors： Castan P,de Pablo A,Fernández-Romero N,Rubio JM,Cobb BD,Mingorance J,Toro C

更新日期：2014-02-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97...

journal_title：Journal of clinical microbiology

authors： Carballo M,Dillon JR,Lussier M,Milthorp P,Winston S,Brodeur B

更新日期：1992-08-01 00:00:00

abstract：:We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was performed on the Light...

journal_title：Journal of clinical microbiology

authors： Leb V,Stöcher M,Valentine-Thon E,Hölzl G,Kessler H,Stekel H,Berg J

更新日期：2004-02-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:Microscopic examination of a permanently stained fecal preparation revealed the unusual inclusion of a Giardia lamblia cyst within the cytoplasm of an Entamoeba coli trophozoite. ...

journal_title：Journal of clinical microbiology

authors： Logan AL,Turner WE,Price J Jr

更新日期：1980-10-01 00:00:00