GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness.

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:We classified microorganisms from the clinical laboratory by using information provided by the Gram stain and antibiotic sensitivity profiles obtained with the Bauer-Kirby technique. Approximately 4,000 microorganisms, routinely identified and tested for antibiotic sensitivities in a large hospital microbiology labora...

journal_title：Journal of clinical microbiology

authors： Boyd JC,Lewis JW,Marr JJ,Harper AM,Kowalski BR

更新日期：1978-12-01 00:00:00

abstract：:Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

journal_title：Journal of clinical microbiology

authors： Vastag M,Papp T,Kasza Z,Vágvölgyi C

更新日期：1998-07-01 00:00:00

abstract：:We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...

journal_title：Journal of clinical microbiology

authors： Klena JD,Parker CT,Knibb K,Ibbitt JC,Devane PM,Horn ST,Miller WG,Konkel ME

更新日期：2004-12-01 00:00:00

abstract：:Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

journal_title：Journal of clinical microbiology

authors： McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

更新日期：2001-01-01 00:00:00

abstract：:We have developed an easy and rapid detection and identification system for the diagnosis of mycobacterial diseases. The system is based on selective amplification by PCR of mycobacteria with primers based on the genes coding for 16S rRNA. During PCR, a label (digoxigenin-11-dUTP) is incorporated with biotinylated spe...

journal_title：Journal of clinical microbiology

authors： De Beenhouwer H,Liang Z,De Rijk P,Van Eekeren C,Portaels F

更新日期：1995-11-01 00:00:00

abstract：:The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement betw...

journal_title：Journal of clinical microbiology

authors： Valu JA

更新日期：1976-02-01 00:00:00

abstract：:Four hundred thirty-two lactose-negative colonies isolated from human feces on stool differential agar media were flooded with one drop of MUCAP Test reagent (Biolife Italiana S.r.I., Milan, Italy) and observed under a Wood lamp for the development of a blue fluorescence over or around the colony. On the basis of manu...

journal_title：Journal of clinical microbiology

authors： Aguirre PM,Cacho JB,Folgueira L,López M,García J,Velasco AC

更新日期：1990-01-01 00:00:00

abstract：:The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

journal_title：Journal of clinical microbiology

authors： Traub WH,Fukushima PI

更新日期：1979-07-01 00:00:00

abstract：:We report a fatal case of acute lower respiratory tract disease with human rhinovirus C (HRV-C) as the unique cause in a 19-month-old girl with a history of repeated episodes of bronchiolitis. HRV-C type 8 nucleic acids were observed in respiratory, stool, and cerebrospinal fluid samples, and infectious virions were i...

journal_title：Journal of clinical microbiology

authors： Lupo J,Schuffenecker I,Morel-Baccard C,Bardet J,Payen V,Kaiser L,Constant S,Lobrinus JA,Lin-Marq N,Lina B,Morand P,Tapparel C

更新日期：2015-05-01 00:00:00

abstract：:Increasing diversity among influenza H5N1 viruses has resulted in the need for sensitive and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all hemagglutinin (HA) clades, particularly the recently circulating H5N1 viruses of clade 2. In this report, the development and validat...

journal_title：Journal of clinical microbiology

authors： Ellis JS,Smith JW,Braham S,Lock M,Barlow K,Zambon MC

更新日期：2007-05-01 00:00:00

abstract：:Rapid identification of pathogens from blood cultures can decrease lengths of stay and improve patient outcomes. We evaluated the accuracy of the Verigene Gram-positive blood culture (BC-GP) nucleic acid test for investigational use only (Nanosphere, Inc., Northbrook, IL) for the identification of Gram-positive bacter...

journal_title：Journal of clinical microbiology

authors： Wojewoda CM,Sercia L,Navas M,Tuohy M,Wilson D,Hall GS,Procop GW,Richter SS

更新日期：2013-07-01 00:00:00

abstract：:The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Lally RT,Ederer MN,Quall CO

更新日期：1984-04-01 00:00:00

abstract：:Spontaneous chylothorax is rare in adults. We present an unusual case that was complicated by Prevotella bivia empyema. Full recovery was achieved with chest tube drainage and prompt treatment with intravenous clindamycin. ...

journal_title：Journal of clinical microbiology

authors： Di Marco Berardino A,Inchingolo R,Smargiassi A,Re A,Torelli R,Fiori B,d'Inzeo T,Corbo GM,Valente S,Sanguinetti M,Spanu T

更新日期：2014-04-01 00:00:00

abstract：:Vibrio cholerae O1 biotype El Tor (ET), the cause of the current 7th pandemic, has recently been replaced in Asia and Africa by an altered ET biotype possessing cholera toxin (CTX) of the classical (CL) biotype that originally caused the first six pandemics before becoming extinct in the 1980s. Until recently, the ET ...

journal_title：Journal of clinical microbiology

authors： Alam M,Nusrin S,Islam A,Bhuiyan NA,Rahim N,Delgado G,Morales R,Mendez JL,Navarro A,Gil AI,Watanabe H,Morita M,Nair GB,Cravioto A

更新日期：2010-10-01 00:00:00

abstract：:This report describes an unusual pathogen associated with post-traumatic meningitis. Haemophilus influenzae type D was isolated from both cerebrospinal fluid and blood of this patient with meningitis. ...

journal_title：Journal of clinical microbiology

authors： Viner JP,Massanari RM

更新日期：1979-02-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:We studied the prevalence and species distribution of nontuberculous mycobacteria (NTM) in relation to age in 385 patients with cystic fibrosis (CF) (mean age +/- standard deviation [range], 12.0 +/- 6.1 [1 to 24] years; sex ratio, 0.53) attending three Parisian centers. The overall prevalence of NTM in sputum was 8.1...

journal_title：Journal of clinical microbiology

authors： Pierre-Audigier C,Ferroni A,Sermet-Gaudelus I,Le Bourgeois M,Offredo C,Vu-Thien H,Fauroux B,Mariani P,Munck A,Bingen E,Guillemot D,Quesne G,Vincent V,Berche P,Gaillard JL

更新日期：2005-07-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:In 1998, 21 inhabitants of a German nursing home fell ill with acute gastroenteritis after consumption of minced beef heart (P. Graf and L. Bader, Epidemiol. Bull. 41:327-329, 2000). Two residents died during hospital treatment. Seventeen Clostridium perfringens strains were collected from two different dishes and fro...

journal_title：Journal of clinical microbiology

authors： Schalch B,Bader L,Schau HP,Bergmann R,Rometsch A,Maydl G,Kessler S

更新日期：2003-02-01 00:00:00

abstract：:Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and fast turnaround time. The Panther Fusion (Fusion; Hologic) system has an array of highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses,...

journal_title：Journal of clinical microbiology

authors： Stellrecht KA,Cimino JL,Maceira VP

更新日期：2020-05-26 00:00:00

abstract：:To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumo...

journal_title：Journal of clinical microbiology

authors： Fenoll A,Jado I,Vicioso D,Casal J

更新日期：1997-03-01 00:00:00

abstract：:A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rR...

journal_title：Journal of clinical microbiology

authors： Carlotti A,Chaib F,Couble A,Bourgeois N,Blanchard V,Villard J

更新日期：1997-06-01 00:00:00

abstract：:Nineteen strains of nutritionally variant streptococci (NVS) were tested for bacteriolytic activity and for their production of and sensitivity to streptococcal bacteriocinlike inhibitory substances (BLIS). None appeared to produce BLIS. An inverse relationship was found between the sensitivity to BLIS and the product...

journal_title：Journal of clinical microbiology

authors： Tagg JR,van de Rijn I

更新日期：1991-04-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:A study of 180 healthy cats found that 15.6% were feline leukemia virus (FeLV) positive, 8.3% were feline immunodeficiency virus (FIV) positive, and 1.1% were FIV and FeLV positive, which corresponded to 30.4, 13.8, and 2.6, of 115 cats with FIV- and FeLV-related symptoms, respectively. Differences were seen in the se...

journal_title：Journal of clinical microbiology

authors： Arjona A,Escolar E,Soto I,Barquero N,Martin D,Gomez-Lucia E

更新日期：2000-09-01 00:00:00

abstract：:Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal ab...

journal_title：Journal of clinical microbiology

authors： Kennedy KJ,Prince S,Makeham T

更新日期：2009-09-01 00:00:00

abstract：:Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

journal_title：Journal of clinical microbiology

authors： Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

更新日期：2005-09-01 00:00:00

abstract：:Respiratory tract infections are a leading cause of morbidity and mortality worldwide. Even with the advancement of diagnostic tools, the causative agent of 20 to 30% of upper respiratory tract infections go undiagnosed. Recently, a newly identified human respiratory virus, human metapneumovirus (hMPV), was discovered...

journal_title：Journal of clinical microbiology

authors： Regev L,Hindiyeh M,Shulman LM,Barak A,Levy V,Azar R,Shalev Y,Grossman Z,Mendelson E

更新日期：2006-04-01 00:00:00