Evaluation of the Verigene Gram-positive blood culture nucleic acid test for rapid detection of bacteria and resistance determinants.

abstract：:Lipooligosaccharide configurations were predicted in nontypeable Haemophilus influenzae isolates based on the presence of seven oligosaccharide extension-initiating genes (or alleles). Predicted configurations with 2 to 3 oligosaccharide extensions were more prevalent among middle ear than throat strains. In addition,...

journal_title：Journal of clinical microbiology

authors： McCrea KW,Xie J,Daniel D,Ulrich-Lewis JT,Zhang L

更新日期：2014-07-01 00:00:00

abstract：:A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard P

更新日期：1978-07-01 00:00:00

abstract：:A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed. ...

journal_title：Journal of clinical microbiology

authors： Kaase M,Szabados F,Wassill L,Gatermann SG

更新日期：2012-09-01 00:00:00

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00

abstract：:The spores of Henneguya salminicola, a common tissue parasite of salmonid fishes in the northern hemisphere, were observed in stool specimens from two different patients with diarrhea. The spores' superficial resemblance to human spermatozoa resulted, in one instance, in an incorrect report, leading to suspicion of se...

journal_title：Journal of clinical microbiology

authors： McClelland RS,Murphy DM,Cone DK

更新日期：1997-11-01 00:00:00

abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

journal_title：Journal of clinical microbiology

authors： Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

更新日期：2018-02-22 00:00:00

abstract：:Developing interpretive breakpoints for any given organism-drug combination requires integration of the MIC distribution, pharmacokinetic and pharmacodynamic parameters, and the relationship between the in vitro activity and outcome from both in vivo and clinical studies. Using data generated by standardized broth mic...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Rex JH,Espinel-Ingroff A,Johnson EM,Andes D,Chaturvedi V,Ghannoum MA,Odds FC,Rinaldi MG,Sheehan DJ,Troke P,Walsh TJ,Warnock DW

更新日期：2006-03-01 00:00:00

abstract：:Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six ot...

journal_title：Journal of clinical microbiology

authors： Miyakawa Y,Kagaya K,Fukazawa Y,Soe G

更新日期：1986-05-01 00:00:00

abstract：:The FUNGITEST method (Sanofi Diagnostics Pasteur, Paris, France) is a microplate-based procedure for the breakpoint testing of six antifungal agents (amphotericin B, flucytosine, fluconazole, itraconazole, ketoconazole, and miconazole). We compared the FUNGITEST method with a broth microdilution test, performed accord...

journal_title：Journal of clinical microbiology

authors： Davey KG,Holmes AD,Johnson EM,Szekely A,Warnock DW

更新日期：1998-04-01 00:00:00

abstract：:All medical and high-risk surgical patients were screened for methicillin-resistant Staphylococcus aureus colonization over 3.5 years. The sensitivities of nasal and rectal swabs were 68% and 62%, respectively. Naris and open-skin-site swabs detected 467 (74%) of 627 adult carriers identified. Rectal swabs detected an...

journal_title：Journal of clinical microbiology

authors： Currie A,Davis L,Odrobina E,Waldman S,White D,Tomassi J,Katz KC

更新日期：2008-09-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00

abstract：:We evaluated three rapid identification systems-The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems-for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigran...

journal_title：Journal of clinical microbiology

authors： LaClaire LL,Facklam RR

更新日期：2000-06-01 00:00:00

abstract：:Twenty-one reference strains and 88 clinical isolates of Aerococcus, Enterococcus, and Streptococcus species were tested for reactions in the Rapid Strep (RS) and modified conventional tests. We conclude that some but not all of the tests in the RS system could be used to substitute for conventional tests. RS tests fo...

journal_title：Journal of clinical microbiology

authors： Fertally SS,Facklam R

更新日期：1987-10-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as ...

journal_title：Journal of clinical microbiology

authors： Alvarez E,Garcia-Hermoso D,Sutton DA,Cano JF,Stchigel AM,Hoinard D,Fothergill AW,Rinaldi MG,Dromer F,Guarro J

更新日期：2010-12-01 00:00:00

abstract：:Chronic infection with hepatitis B virus (HBV) is an important cause of cirrhosis and cancer of the liver. HBV is currently classified into eight genotypes, A to H. Accumulated evidence shows that the genotype influences both the clinical course of infection and the response to treatment. We describe a new method for ...

journal_title：Journal of clinical microbiology

authors： Malmström S,Berglin-Enquist I,Lindh M

更新日期：2010-04-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides th...

journal_title：Journal of clinical microbiology

authors： Kaman WE,Galassi F,de Soet JJ,Bizzarro S,Loos BG,Veerman EC,van Belkum A,Hays JP,Bikker FJ

更新日期：2012-01-01 00:00:00

abstract：:Rotavirus is a leading cause of dehydrating diarrhea and death among infants and children globally, particularly in communities of the developing world. While numerous studies have described the complex relationships among infectious diarrhea, growth faltering, and poverty, the impact of nutritional status on suscepti...

journal_title：Journal of clinical microbiology

authors： Verkerke H,Sobuz S,Ma JZ,Petri SE,Reichman D,Qadri F,Rahman M,Haque R,Petri WA Jr

更新日期：2016-10-01 00:00:00

abstract：:A young immunocompetent patient was admitted for a febrile illness with malaise, arthralgias, painful leg swelling, and polyserositis. Shortly prior to becoming ill, the patient had traveled to the Northern African desert. The symptoms disappeared during treatment with antibiotics (doxycycline and ceftriaxone) but rec...

journal_title：Journal of clinical microbiology

authors： Iten A,Graf S,Egger M,Täuber M,Graf J

更新日期：2001-05-01 00:00:00

abstract：:Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor...

journal_title：Journal of clinical microbiology

authors： Salam MA,Khan MG,Bhaskar KR,Afrad MH,Huda MM,Mondal D

更新日期：2012-03-01 00:00:00

abstract：:Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...

journal_title：Journal of clinical microbiology

authors： White LA,Ades EW

更新日期：1990-02-01 00:00:00

abstract：:This study demonstrates the dynamics in the epidemiology of hepatitis C virus subtypes. Subtypes 3a and 4a have become increasingly prevalent in patients where an infection within recent years can be assumed. Evidence is presented that the subtypes observed among younger patients can spread rapidly and lead to signifi...

journal_title：Journal of clinical microbiology

authors： Schröter M,Zöllner B,Schäfer P,Reimer A,Müller M,Laufs R,Feucht HH

更新日期：2002-05-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:In the SIGNAL (Oxoid Ltd., Basingstoke, United Kingdom) blood culture system, gas produced by bacterial metabolism displaces medium from the culture bottle into an upper reservoir via a hollow needle. Displacement of media may provide a visual indication of the presence of both aerobic and anaerobic organisms in a sin...

journal_title：Journal of clinical microbiology

authors： Trombley C,Anderson JD

更新日期：1987-11-01 00:00:00

abstract：:Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

journal_title：Journal of clinical microbiology

authors： Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

更新日期：1991-09-01 00:00:00

abstract：:Given that the integration of human papillomavirus type 16 (HPV16) into the host genome occurs preferentially with the disruption of the E2 gene, a ratio of E2 to E7 gene copies is often used as a marker for integration. It is largely undetermined, however, whether ratio estimates are affected by HPV intratypic variat...

journal_title：Journal of clinical microbiology

authors： Jiang M,Baseman JG,Koutsky LA,Feng Q,Mao C,Kiviat NB,Xi LF

更新日期：2009-03-01 00:00:00

abstract：:Diagnosis of Bordetella pertussis infection has been difficult due to the low sensitivity of culture. PCR tests have been shown to be more sensitive than culture, but the reported sensitivity of PCR is variable. We evaluated PCR product detection by using either agarose gel electrophoresis (PCR-gel) or dot blot hybrid...

journal_title：Journal of clinical microbiology

authors： Lingappa JR,Lawrence W,West-Keefe S,Gautom R,Cookson BT

更新日期：2002-08-01 00:00:00

abstract：:CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast direct...

journal_title：Journal of clinical microbiology

authors： Horvath LL,Hospenthal DR,Murray CK,Dooley DP

更新日期：2003-06-01 00:00:00