Report of spores of Henneguya salminicola (Myxozoa) in human stool specimens: possible source of confusion with human spermatozoa.

abstract：:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

journal_title：Journal of clinical microbiology

authors： Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

更新日期：2007-08-01 00:00:00

abstract：:We have evaluated the KOH test, the antibiotic disk identification test, and the Gram stain reaction for the preliminary grouping of gram-positive and gram-negative anaerobes and have assessed the value of erythromycin 60-micrograms-disk resistance as a predictive index of clindamycin resistance among anaerobes. By te...

journal_title：Journal of clinical microbiology

authors： Bourgault AM,Lamothe F

更新日期：1988-10-01 00:00:00

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...

journal_title：Journal of clinical microbiology

authors： Rodriguez E,De Meeüs T,Mallie M,Renaud F,Symoens F,Mondon P,Piens MA,Lebeau B,Viviani MA,Grillot R,Nolard N,Chapuis F,Tortorano AM,Bastide JM

更新日期：1996-10-01 00:00:00

abstract：:Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

journal_title：Journal of clinical microbiology

authors： Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

更新日期：2015-07-01 00:00:00

abstract：:The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laborato...

journal_title：Journal of clinical microbiology

authors： Rennie RP,Brosnikoff C,Turnbull L,Reller LB,Mirrett S,Janda W,Ristow K,Krilcich A

更新日期：2008-08-01 00:00:00

abstract：:Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental carie...

journal_title：Journal of clinical microbiology

authors： Becker MR,Paster BJ,Leys EJ,Moeschberger ML,Kenyon SG,Galvin JL,Boches SK,Dewhirst FE,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:Whole HIV-1 genome sequences are pivotal for large-scale studies of inter- and intrahost evolution, including the acquisition of drug resistance mutations. The ability to rapidly and cost-effectively generate large numbers of HIV-1 genome sequences from different populations and geographical locations and determine th...

journal_title：Journal of clinical microbiology

authors： Gall A,Ferns B,Morris C,Watson S,Cotten M,Robinson M,Berry N,Pillay D,Kellam P

更新日期：2012-12-01 00:00:00

abstract：:Current methods of DNA extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. DNA isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. We therefore investigated a new and rapid DNA isolation metho...

journal_title：Journal of clinical microbiology

authors： Müller FM,Werner KE,Kasai M,Francesconi A,Chanock SJ,Walsh TJ

更新日期：1998-06-01 00:00:00

abstract：:Tick-borne ehrlichial pathogens of animals and humans require a mammalian reservoir of infection from which ticks acquire the organism for subsequent transmission. In the present study, we examined the strain structure of Anaplasma marginale, a genogroup II ehrlichial pathogen, in both an acute outbreak and in persist...

journal_title：Journal of clinical microbiology

authors： Palmer GH,Rurangirwa FR,McElwain TF

更新日期：2001-02-01 00:00:00

abstract：:Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

journal_title：Journal of clinical microbiology

authors： Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

更新日期：1999-12-01 00:00:00

abstract：:Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms wer...

journal_title：Journal of clinical microbiology

authors： Chambers CA,Silver WS

更新日期：1977-11-01 00:00:00

abstract：:Two new T-cell-based reporter cell lines were established to measure human immunodeficiency virus type 1 (HIV-1) infectivity. One cell line naturally expresses CD4 and CXCR4, making it susceptible to X4-tropic viruses, and the other cell line, in which a CCR5 expression vector was introduced, is susceptible to both X4...

journal_title：Journal of clinical microbiology

authors： Chiba-Mizutani T,Miura H,Matsuda M,Matsuda Z,Yokomaku Y,Miyauchi K,Nishizawa M,Yamamoto N,Sugiura W

更新日期：2007-02-01 00:00:00

abstract：:A washing procedure that removed maximum numbers of contaminating microorganisms from whole surgical gloves was developed. Washing, coupled with membrane filtration, proved to be a simple and effective method for bioburden determinations on whole gloves. ...

journal_title：Journal of clinical microbiology

authors： Lammerding AM,Day DF

更新日期：1978-05-01 00:00:00

abstract：:Johne's disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis As an alternative to serological tests, which are used mainly for the screening of whole herds, we developed a novel ResoLight-based real-time PCR (RL-PCR) assay with pooled f...

journal_title：Journal of clinical microbiology

authors： Kawaji S,Nagata R,Minegishi Y,Saruyama Y,Mita A,Kishizuka S,Saito M,Mori Y

更新日期：2020-11-18 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes is...

journal_title：Journal of clinical microbiology

authors： Bosi C,Davin-Regli A,Bornet C,Mallea M,Pages JM,Bollet C

更新日期：1999-07-01 00:00:00

abstract：:The BBL Crystal system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a miniaturized bacterial identification method employing modified conventional and chromogenic substrates. Two products are currently available, the Rapid Stool/Enteric ID Kit and the Enteric/Nonfermenter ID Kit, each comprising thirt...

journal_title：Journal of clinical microbiology

authors： Holmes B,Costas M,Thaker T,Stevens M

更新日期：1994-09-01 00:00:00

abstract：:The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of th...

journal_title：Journal of clinical microbiology

authors： Lan X,Yang B,Li BY,Yin XP,Li XR,Liu JX

更新日期：2009-07-01 00:00:00

abstract：:Candida famata (teleomorph Debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. Among 53 strains collected during the SENTRY and ARTEMIS surveillance programs and previousl...

journal_title：Journal of clinical microbiology

authors： Castanheira M,Woosley LN,Diekema DJ,Jones RN,Pfaller MA

更新日期：2013-01-01 00:00:00

abstract：:The sensitivity and specificity of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay (BED-CEIA) were compared with those of the avidity index method to identify recent HIV infection using a panel of 148 samples (81 patients) representing durations of infe...

journal_title：Journal of clinical microbiology

authors： Loschen S,Bätzing-Feigenbaum J,Poggensee G,Cordes C,Hintsche B,Rausch M,Dupke S,Gohlke-Micknis S,Rödig J,Hamouda O,Kücherer C

更新日期：2008-01-01 00:00:00

abstract：:Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

journal_title：Journal of clinical microbiology

authors： Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

更新日期：2012-02-01 00:00:00

abstract：:Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals, who may develop a progressive disseminated form which is often fatal if it is untreated. In such patients, the detection of antibody responses for both diagnosis and follow-up may be of limited use, whereas the ...

journal_title：Journal of clinical microbiology

authors： Gómez BL,Figueroa JI,Hamilton AJ,Diez S,Rojas M,Tobón A,Restrepo A,Hay RJ

更新日期：1999-03-01 00:00:00

abstract：:In this study, we determined the sequence of the EcoRI-PstI fragment of the plasmid pCVD432, also termed the enteroaggregative Escherichia coli (EAggEC) probe. A primer pair complementary to this probe was designed for PCR amplification of a 630-bp region. Comparison of the analysis of the EAggEC probe sequence with t...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Knop C,Franke S,Aleksic S,Heesemann J,Karch H

更新日期：1995-03-01 00:00:00

abstract：:Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...

journal_title：Journal of clinical microbiology

authors： Aoki FH,Imai T,Tanaka R,Mikami Y,Taguchi H,Nishimura NF,Nishimura K,Miyaji M,Schreiber AZ,Branchini ML

更新日期：1999-02-01 00:00:00

abstract：:A new case of Campylobacter hyointestinalis-associated diarrhea in a human is reported. The medical history of the patient was significant for immunodeficiency because of an evolutive chronic myeloid leukemia. The diarrhea rapidly stopped after administration of oral erythromycin. No other enteropathogenic agent was f...

journal_title：Journal of clinical microbiology

authors： Minet J,Grosbois B,Megraud F

更新日期：1988-12-01 00:00:00

abstract：:Human papillomavirus (HPV) DNA detection and typing are important for diagnosis and management of HPV-associated diseases. One of the most commonly used PCR methods, GP5+/6+, shows weaknesses in amplifying certain types. To circumvent this limitation, we developed and validated broad-spectrum primers targeting the GP5...

journal_title：Journal of clinical microbiology

authors： Schmitt M,Dondog B,Waterboer T,Pawlita M

更新日期：2008-03-01 00:00:00