Abstract:
:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan virus. Sixty-two serum samples from 15 patients with clinically typical nephropathia epidemica were analyzed. Eleven patients had specific IgM in one or more serum samples. The IgM could be demonstrated from day 2 up to day 37, and all patients had detectable specific IgM within 15 days after the onset of disease. In 49 control serum samples, no specific IgM could be detected, indicating a high specificity for the method. The findings demonstrate that the biotin-avidin-amplified immunofluorescence IgM assay is a useful tool in the diagnosis of early nephropathia epidemica disease.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Settergren B,Juto P,Wadell Gdoi
10.1128/JCM.25.6.1134-1136.1987subject
Has Abstractpub_date
1987-06-01 00:00:00pages
1134-6issue
6eissn
0095-1137issn
1098-660Xjournal_volume
25pub_type
杂志文章abstract::We report the first documented case of canine strangles due to infection with Streptococcus equi in a dog with enlarged lymph nodes. Genetic typing, via sequencing of 12 housekeeping genes and the SeM gene, demonstrated the isolate to be a member of a common equine strain type circulating in the United Kingdom. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00571-06
更新日期:2006-07-01 00:00:00
abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.06701-11
更新日期:2012-05-01 00:00:00
abstract::Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tic...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00147-20
更新日期:2020-10-21 00:00:00
abstract::We report a fatal case of acute lower respiratory tract disease with human rhinovirus C (HRV-C) as the unique cause in a 19-month-old girl with a history of repeated episodes of bronchiolitis. HRV-C type 8 nucleic acids were observed in respiratory, stool, and cerebrospinal fluid samples, and infectious virions were i...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.03484-14
更新日期:2015-05-01 00:00:00
abstract::Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.8.1793-1796.1989
更新日期:1989-08-01 00:00:00
abstract::A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.10.2300-2304.1989
更新日期:1989-10-01 00:00:00
abstract::We have previously demonstrated homology between a 181-bp fragment of IS6110 and DNA from mycobacteria other than tuberculosis-causing mycobacteria (MOTT). Genomic DNA from 14 strains of MOTT was digested with PvuII and was hybridized with a probe derived from the 181-bp fragment and the INS1/INS2 international standa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.7.1769-1771.1997
更新日期:1997-07-01 00:00:00
abstract::The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01979-16
更新日期:2016-12-28 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.8.2674-2677.1999
更新日期:1999-08-01 00:00:00
abstract::Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic acid amplification tests (NAATs). The objective was to determine whether VS are equal to Food and Drug Administration (FDA...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/jcm.41.8.3784-3789.2003
更新日期:2003-08-01 00:00:00
abstract::The prevalence rates of Mycoplasma genitalium infections and coinfections with other sexually transmitted organisms and the frequency of a macrolide antibiotic resistance phenotype were determined in urogenital specimens collected from female and male subjects enrolled in a multicenter clinical study in the United Sta...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.01053-16
更新日期:2016-09-01 00:00:00
abstract::A sandwich enzyme-linked immunosorbent assay was developed for its potential utility in the detection of antigen in the cerebrospinal fluid of patients with tuberculous meningitis. Cerebrospinal fluids examined included those from untreated (group Ia) and treated (group Ib) Mycobacterium tuberculosis meningitis, nonse...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.5.901-904.1986
更新日期:1986-05-01 00:00:00
abstract::Human group C rotavirus was identified in central Australia in each of eight years over a 16-year period between 1982 and 1997. Cases occurred either sporadically but over a relatively short period of time or as clustered outbreaks. These are the only reports of human group C rotavirus in Australia other than that of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.42.5.2127-2133.2004
更新日期:2004-05-01 00:00:00
abstract::Following intranasal administration of live influenza A virus vaccine or parenteral inoculation of inactivated influenza virus vaccine, immunoglobulin antibody to the influenza virus hemagglutinin was detected in nasal wash specimens from adult volunteers. Several observations supported the suggestion that this immuno...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.3.559-562.1987
更新日期:1987-03-01 00:00:00
abstract::A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.2.254-257.1983
更新日期:1983-08-01 00:00:00
abstract::Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.10.2844-2846.1998
更新日期:1998-10-01 00:00:00
abstract::Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.8.2118-2123.1993
更新日期:1993-08-01 00:00:00
abstract::Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::There are no previous reports of human infection due to Acinetobacter baylyi. In this study, we report on six patients with bacteremia due to A. baylyi, based on analysis of the 16S-23S rRNA intergenic spacer and the 16S rRNA gene. All six patients had multiple underlying diseases. The infection was nosocomially acqui...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00232-08
更新日期:2008-09-01 00:00:00
abstract::To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.4.734-737.1990
更新日期:1990-04-01 00:00:00
abstract::Cellulosimicrobium funkei is a rare, opportunistic pathogen. We describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. Useful phenotypic tests for differentiating C. funkei from Cellulosimicrobium cellulans and Cellulosimicrobium terreum include mot...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01103-10
更新日期:2011-03-01 00:00:00
abstract::The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study perio...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.4.1525-1528.2003
更新日期:2003-04-01 00:00:00
abstract::During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.63-67.1983
更新日期:1983-01-01 00:00:00
abstract::The BBL Crystal system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) is a miniaturized bacterial identification method employing modified conventional and chromogenic substrates. Two products are currently available, the Rapid Stool/Enteric ID Kit and the Enteric/Nonfermenter ID Kit, each comprising thirt...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.9.2221-2224.1994
更新日期:1994-09-01 00:00:00
abstract::In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.4.631-635.1983
更新日期:1983-04-01 00:00:00
abstract::The genetic diversity of 123 Streptococcus suis strains of capsular types 2, 1/2, 3, 7, and 9, isolated from pigs in France and from humans in different countries, was evaluated by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI. The method was highly discriminative (D = 0.98), results were reprodu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.2.615-6192002
更新日期:2002-02-01 00:00:00
abstract::Since the late 1980s several studies have described the increased incidence and severity of invasive group A streptococcal (GAS) infections. However, most studies on GAS pathogenesis have focused on information obtained during outbreaks. We analyzed isolate distribution and host susceptibility as part of a nationwide ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.7.3101-3109.2005
更新日期:2005-07-01 00:00:00
abstract::We compared trends of annual resistance rates calculated from results for all isolates and for the first isolate of Staphylococcus aureus, Klebsiella pneumoniae, and Acinetobacter baumannii per patient over a 3-year period from 2001 through 2003. Antimicrobial susceptibility results of inpatients were extracted from a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.10.4776-4779.2004
更新日期:2004-10-01 00:00:00
abstract::We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the ne...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00851-10
更新日期:2010-11-01 00:00:00
abstract::Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.9.2381-2386.1993
更新日期:1993-09-01 00:00:00