Sensitivity and specificity of enzyme-linked immunosorbent assay in the detection of antigen in tuberculous meningitis cerebrospinal fluids.

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...

journal_title：Journal of clinical microbiology

authors： Shyamala GN,Roberton DM,Hosking CS

更新日期：1988-08-01 00:00:00

abstract：:All medical and high-risk surgical patients were screened for methicillin-resistant Staphylococcus aureus colonization over 3.5 years. The sensitivities of nasal and rectal swabs were 68% and 62%, respectively. Naris and open-skin-site swabs detected 467 (74%) of 627 adult carriers identified. Rectal swabs detected an...

journal_title：Journal of clinical microbiology

authors： Currie A,Davis L,Odrobina E,Waldman S,White D,Tomassi J,Katz KC

更新日期：2008-09-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:Neutralizing serum antibody to serotype 9 rotavirus WI61 was detected in 41% of 870 Ecuadorian children and 26% of 140 German children. In both areas an age-related prevalence increase was observed. We identified 11 serum samples from Ecuadorian children which neutralized exclusively serotype 9 rotavirus. Thirteen of ...

journal_title：Journal of clinical microbiology

authors： Brüssow H,Clark HF,Sidoti J

更新日期：1991-01-01 00:00:00

abstract：:A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inac...

journal_title：Journal of clinical microbiology

authors： Christianson GG,Pemberton JR,Koski TA

更新日期：1980-04-01 00:00:00

abstract：:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

journal_title：Journal of clinical microbiology

authors： Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

更新日期：2014-12-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of PT in control experiments and to test ...

journal_title：Journal of clinical microbiology

authors： Friedman RL,Paulaitis S,McMillan JW

更新日期：1989-11-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) is still a major problem in routine clinical practice. Early diagnosis is essential for a good patient prognosis. PCR is a highly sensitive method for the detection of nucleic acids and could play an important role in improving the diagnosis of fungal infections. Therefore, a n...

journal_title：Journal of clinical microbiology

authors： Springer J,Schloßnagel H,Heinz W,Doedt T,Soeller R,Einsele H,Loeffler J

更新日期：2012-08-01 00:00:00

abstract：:A cytochrome b (cytb) gene quantitative PCR (qPCR) assay was developed to diagnose malaria in travelers. First, manual and automated DNA extractions were compared and automated DNA extraction of 400 μl of blood was found to be more efficient. Sensitivity was estimated using the WHO international standard for Plasmodiu...

journal_title：Journal of clinical microbiology

authors： Farrugia C,Cabaret O,Botterel F,Bories C,Foulet F,Costa JM,Bretagne S

更新日期：2011-06-01 00:00:00

abstract：:The effectiveness of antiretroviral therapy may be limited by the development of human immunodeficiency virus type 1 (HIV-1) resistance. Monitoring for resistance will perhaps allow changes in therapy prior to deterioration in the patient's clinical or immunologic status. Our objective was to develop a rapid, specific...

journal_title：Journal of clinical microbiology

authors： Frenkel LM,Wagner LE 2nd,Atwood SM,Cummins TJ,Dewhurst S

更新日期：1995-02-01 00:00:00

abstract：:The Alere i Influenza A&B assay is a newly developed rapid molecular assay which has the potential to generate results within 15 min from sample collection. In this study, we evaluated the Alere i Influenza A&B assay by using salvaged frozen respiratory specimens that were collected in viral transport medium from chil...

journal_title：Journal of clinical microbiology

authors： Bell JJ,Selvarangan R

更新日期：2014-11-01 00:00:00

abstract：:Methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. We describe here a 2-h identification system for mycobacterial isolates belonging to the Mycobacterium tuberculosis complex using a DNA probe. After 30 min of hands...

journal_title：Journal of clinical microbiology

authors： Mabilat C,Desvarenne S,Panteix G,Machabert N,Bernillon MH,Guardiola G,Cros P

更新日期：1994-11-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:We describe a central venous catheter-related (Port-A-Cath; Smiths Industries Medical Systems [SIMS] Deltec, Inc., St. Paul, Minn.) infection caused by Rhodotorula glutinis in a 51-year-old man with nasopharyngeal carcinoma. He was treated with fluconazole for 8 weeks and had the catheter removed. Two isolates of R. g...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Ho SW,Luh KT

更新日期：2003-02-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...

journal_title：Journal of clinical microbiology

authors： Huang B,Jin D,Zhang J,Sun JY,Wang X,Stiles J,Xu X,Kamboj M,Babady NE,Tang YW

更新日期：2014-04-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

journal_title：Journal of clinical microbiology

authors： Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

更新日期：2015-02-01 00:00:00

abstract：:Capnocytophaga is a genus of gram-negative rods involved mainly in bacteremia in immunocompromised hosts. Here we report a case of Capnocytophaga peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. The disease appeared as a superinfection during antimicrobial therapy of a previous episode of...

journal_title：Journal of clinical microbiology

authors： Esteban J,Albalate M,Caramelo C,Reyero A,Carriazo MA,Hernandez J,Ortiz A,Soriano F

更新日期：1995-09-01 00:00:00

abstract：:A total of 46 Borrelia burgdorferi sensu lato isolates that were isolated from patients with Lyme borreliosis and infected animals or were extracted from ticks of the genus Ixodes were analyzed. Large restriction fragment patterns obtained after cleavage of genomic DNAs with MluI were analyzed by pulsed-field gel elec...

journal_title：Journal of clinical microbiology

authors： Belfaiza J,Postic D,Bellenger E,Baranton G,Girons IS

更新日期：1993-11-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:PCR was performed for the detection of Helicobacter pylori in feces from 24 patients with proven infections. Several precautions were taken to overcome possible inhibition of PCR with feces. In the first 12 patients, feces were examined shortly after endoscopy. In another group of 12 patients, who were treated during ...

journal_title：Journal of clinical microbiology

authors： van Zwet AA,Thijs JC,Kooistra-Smid AM,Schirm J,Snijder JA

更新日期：1994-05-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subje...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Iona E,Arzumanian E,Checchi F,Oggioni MR,Orefici G,Fattorini L

更新日期：2005-01-01 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:Matched vaginal and cervical specimens from 96 subjects were analyzed by quantitative PCR for the presence and concentration of bacterial vaginosis-associated microbes and commensal Lactobacillus spp. Detection of these microbes was 92% concordant, indicating that microbial floras at these body sites are generally sim...

journal_title：Journal of clinical microbiology

authors： Smith WL,Hedges SR,Mordechai E,Adelson ME,Trama JP,Gygax SE,Kaunitz AM,Hilbert DW

更新日期：2014-08-01 00:00:00

abstract：:Herpes simplex virus type 2 (HSV-2) seroprevalence in 68 patients with AIDS was 77% by Western blot (immunoblot) and 44% by glycoprotein G-2 immunoassay. Each of 16 patients with culture-proven HSV-2 infection was positive by Western blot versus 8 by glycoprotein G-2 immunoassay. No differences in age, race, duration ...

journal_title：Journal of clinical microbiology

authors： Safrin S,Arvin A,Mills J,Ashley R

更新日期：1992-05-01 00:00:00

abstract：:Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manne...

journal_title：Journal of clinical microbiology

authors： Beck ET,Buchan BW,Riebe KM,Alkins BR,Pancholi P,Granato PA,Ledeboer NA

更新日期：2014-06-01 00:00:00

abstract：:Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16...

journal_title：Journal of clinical microbiology

authors： Kostman JR,Edlind TD,LiPuma JJ,Stull TL

更新日期：1992-08-01 00:00:00