Abstract:
:In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey isolates displayed both type I and type II colonial morphologies on crystal violet erythromycin agar, whereas the canine isolates displayed only type I. Antigen preparations of the human isolates, typed strains, and two of the monkey strains shared lines of identity in immunodiffusion testing. An enzyme-linked immunosorbent assay in which rabbit anti-F. nucleatum and human sera were used revealed that the human and monkey isolates, when used as antigens, allowed the detection of similar degrees of antibody activity; however, no activity was observed with the canine isolates. None of the canine isolates displayed the characteristic hemagglutination of sheep erythrocytes demonstrated by the human and monkey strains. These results suggest that F. nucleatum isolates from various human periodontal diseases share antigenic determinants. Also, differences were observed in F. nucleatum isolates from canines when compared with human and monkey strains. These findings are important when considering the immunopathological mechanisms involved in human periodontal diseases and when comparing animal model systems with naturally occurring disease in humans.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Vincent JW,Falkler WA Jr,Craig JAdoi
10.1128/JCM.17.4.631-635.1983subject
Has Abstractpub_date
1983-04-01 00:00:00pages
631-5issue
4eissn
0095-1137issn
1098-660Xjournal_volume
17pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JCM.30.12.3288-3289.1992
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.11.4.424-425.1980
更新日期:1980-04-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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更新日期:2003-05-01 00:00:00
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更新日期:2003-04-01 00:00:00
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.11.3742-3745.1999
更新日期:1999-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1984-10-01 00:00:00